We narrowed to 348 results for: abo.4

...harvested and purified. In total, this process takes 4-7 days, not counting the time needed to expand the...completed in one long day, or the virus can be stored at 4 °C and buffer exchanged the next day. Refer to the...a workspace with plasmid, not that I would know about this… A no template control (NTC) should always ... variability. Check out this post to learn more about different ways to titer AAV. Do you have any AAV...at Fred Hutchinson Cancer Center for teaching me about AAV production and purification! References 1. Aurnhammer...

...designed to detect resulting in false positives [2, 4]. Solutions to the polymer problem There are some ..., 1999. 17(10): p. 969-73. PubMed PMID: 10504696. 4. Ohashi, T., et al., An experimental study of GFP-...sold by Clontech and is in a freezer box in just about every lab in the world, is not inert. In all seriousness....11 mM [2]. Following the simple logic outlined above, you might think EGFP is unlikely to form dimers...affinity assays used in the papers describing the FPs above including molecular sizing columns and native gels...

...plant synthetic biology’, Frontiers in Plant Science, 4. doi: 10.3389/fpls.2013.00339. PubMed PMID: 24032037...PMID: 25902487. PubMed Central PMCID: PMC4426443. 4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary... J. (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https...https://books.google.at/books/about/Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September ...

...fusions to 3 or more HA tags. 4. High-resolutions microscopy As indicated above, smFPs can be used with conventional...2009.12 (2009): pdb-top63. PubMed PMID: 20150100. 4. Shaner, Nathan C., George H. Patterson, and Michael...enable accurate labeling of neurons. As discussed above, weakly expressed proteins in particular are difficult...resembles images of the deity (see comparison image above). The church of the Flying Spaghetti Monster is ...

...scaffolds for cell-specific gene manipulation.” Cell 154(4) (2013): 928-939. PubMed PMID: 23953120. PubMed Central... Cepko. Listen to the podcast below to hear all about the research being conducted in Professor Cepko'...and mentoring styles. If you'd like to learn more about Professor Cepko's recent work on developing GFP-...the time to talk to us and look forward to hear about more exciting discoveries from her lab. Special ...

...cohesive end ligation by this enzyme for all 3- and 4-base overhang sequences under standard reaction conditions...identification of mismatch-prone pairings. Using these 4 bp overhang observations, accurate “high-fidelity”...Inserts, either precloned or in amplicon form (shown above) are flanked by BsaI sites in an orientation that...Check out NEB's work on ligase efficiency Learn about other plasmid cloning techniques Resources on Addgene.org...

...exhibit increased avidity to high profile antigens; and 4) generating mAbs against new cancer biomarkers. We...antibodies to be used as a powerful reagent in the laboratory.   The DSHB focuses on monoclonal antibodies,...Hybridoma Bank (DSHB) since 1996.       More Blog Posts about Scientific Sharing: The 10 Most Distributed Plasmid...

...lentiviral packaging as a single payload (Figure 4). Figure 4: ONE-GO biosensors measure the formation...synaptic events By Brian O’Neill The Lin Lab and collaborators recently created ASAP5, a faster and more responsive...

...Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927. https://doi.org/10.1016/J.CELL.2013.07.018... 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J., & Liu, D. R. (2023). Prime editing for... al., 2023). We trained our models in close collaboration with the Krauthammer lab (University of Zurich...

...:3499-3510. doi: 10.1002/bit.27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley...absorption coefficient of a protein. Protein Sci. 1995 Nov;4(11):2411-23. doi: 10.1002/pro.5560041120. PMID: 8563639...validated, it’s ready for use! You can learn more about common antibody applications in our Antibodies 101...

...buffer. Additionally, protein lysis should be done at 4 °C or on ice, whichever is more practical. If your...protein-based blockers degrade fairly quickly, even at 4 °C, so when in doubt, make up a fresh batch.  Antibodies...temperature and time. Though the most common conditions are 4 °C overnight or room temperature for 1–2 hours, there...protein/sample loaded Incubate primary antibody at 4 °C overnight Incomplete washing Ensuring wash...re in the right place! In this post, we’ll talk about western blot optimization and troubleshooting.   ...can be informative, so spend some time thinking about which type of gradient can help answer your particular...overexpression. Before starting a western blot, think about how you’ll interpret the data and what level of ...

...tech transfer and the adoption of the UBMTA Chapter 4 - Developing Addgene as a modern e-commerce company...repository’s future. If you’ve ever been curious about what it takes to start your own nonprofit, this ...Harvard University, where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge...

...of 39 Scientific Impact Measures" (2009) PLOS One 4(6): e6022. doi: 10.1371/journal.pone.0006022 Aragon...Of course, all of you toiling away in laboratories this holiday season want the work you are doing to... has to do with online accessibility. It is now about as easy to find a paper in a lesser-known journal...

...in labcoats (@ladiesinlabcoats) on Dec 19, 2018 at 4:36am PST Buddy the Elf from Matthew Sikora’s lab...more (stay tuned for a few blog posts coming up about sustainability in the lab!). The @ladiesinlabcoats...

...the sequence isn’t in place yet.     Figure 4: S. pyogenes Cas9 in the apo state (PDB ID 4CMP, top...641–656. https://doi.org/10.1038/s41579-022-00739-4 References Bravo, J. P. K., Liu, M.-S., Hibshman, ...sequence. Nature Structural & Molecular Biology, 29(4), 395–402. https://doi.org/10.1038/s41594-022-00756...nascent heteroduplex and abolish cleavage (Pattanayak et al., 2013). As mentioned above, however, Cas9 sometimes..., 2022), indicating that there is more to learn about this process!      Figure 3: Crystal structure...rest of Cas9 and is highly flexible; it can rotate about 140 degrees between inactive and active conformations...conformational changes that make Cas9 such a valuable laboratory tool are still an area of active research. New...

... importantly, it’ll help you define salary costs. 4. Ascribe a cost structure associated to these tools...others will help prove that you will be an asset. 4. Always ask, “Is there an alternative?” As scientists...a successful negotiation and collaboration. We’d love to hear more about your experiences and opinions...can get away with using computer modeling and collaborations rather than using animals, then that’s a cost...associated with old equipment, like service repairs, labour costs, and loss of time, can exceed the cost of...Everything is negotiable. Try asking for discounts, collaborations, access to internal award applications, etc...This is a non-profit organization. Therefore, collaboration and community is the ultimate value proposition...

...the contact to start a direct collaboration between researcher and expert; 4) Credit all contributions. ...our blog post about remote collaborations Read our Transferable Skills Guide article about external collaborations...needed. In the example above, for example, we fostered a formal scientific collaboration between two research...project. We think that these collaborations add enormous value. A discussion about a protocol can save weeks..., the researchers did not have access to a P3 laboratory (BSL-3), a high-security facility needed to perform...approach was useful in humans. Then, one of them heard about our online platform, called Crowdfight, and filed...just the right infrastructure and offered to collaborate with them. The partnership was fruitful and a...

...efficacy of your antibody. When compared, 4% PFA and 0.1% saponin; 4% PFA and 0.1% Triton X-100; and methanol...and KO cells were mixed in a 1:1 ratio and fixed in 4% PFA and permeabilized in 0.1% saponin. 400,000 cells...leukocytes. The file shared also contains information about the labeling pattern of several cell lines using...Laflamme, C., Virk, H., NeuroSGC/YCharOS/EDDU Collaborative Group, & ABIF Consortium. (2024). A guide to...

...million views from 119 different countries (Figure 4). Four of the five most popular videos are protocol...other videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel,...a less tangible form: knowledge and information about the materials in our repository. We started to increase...by making it easier to find materials and learn about them. As early as 2007, we began grouping similar...professors, stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to...seeing our resources shared via social media, on collaborators’ websites, or as a recommendation in a Reddit...updating and expanding our website, we’ve learned about the importance of smaller, systemic changes, like...

...and AAV1 are the most requested serotypes (Figure 4), our catalog includes vectors in a variety of serotypes... and the systemic capsid PHP.eB.   Figure 4: A) Cumulative AAV preps for the seven most popular...all, a delivery method. I would be remiss to talk about them without looking at what they’re delivering .... But in the years since, we’ve learned so much about what it takes to make these viral vectors not only...., 2016), video, educational resources, and collaborations, and will continue to do so, in the hopes that...leave a comment; or take our AAV survey. Learn more about GCaMPs and other tools developed through the GENIE...