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We narrowed to 340 results for: abo.4

Showing: 121 - 140 of 340 results

  1. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...plant synthetic biology’, Frontiers in Plant Science, 4. doi: 10.3389/fpls.2013.00339. PubMed PMID: 24032037...PMID: 25902487. PubMed Central PMCID: PMC4426443. 4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary... J. (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https...https://books.google.at/books/about/Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September ...

  2. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...fusions to 3 or more HA tags. 4. High-resolutions microscopy As indicated above, smFPs can be used with conventional...2009.12 (2009): pdb-top63. PubMed PMID: 20150100. 4. Shaner, Nathan C., George H. Patterson, and Michael...enable accurate labeling of neurons. As discussed above, weakly expressed proteins in particular are difficult...resembles images of the deity (see comparison image above). The church of the Flying Spaghetti Monster is ...

  3. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

    Type
    Blog Post
    Published
    Dec. 8, 2015, 3:30 p.m.
    ...scaffolds for cell-specific gene manipulation.” Cell 154(4) (2013): 928-939. PubMed PMID: 23953120. PubMed Central... Cepko. Listen to the podcast below to hear all about the research being conducted in Professor Cepko'...and mentoring styles. If you'd like to learn more about Professor Cepko's recent work on developing GFP-...the time to talk to us and look forward to hear about more exciting discoveries from her lab. Special ...

  4. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...cohesive end ligation by this enzyme for all 3- and 4-base overhang sequences under standard reaction conditions...identification of mismatch-prone pairings. Using these 4 bp overhang observations, accurate “high-fidelity”...Inserts, either precloned or in amplicon form (shown above) are flanked by BsaI sites in an orientation that...Check out NEB's work on ligase efficiency Learn about other plasmid cloning techniques Resources on Addgene.org...

  5. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    Published
    March 10, 2015, 2:25 p.m.
    ...exhibit increased avidity to high profile antigens; and 4) generating mAbs against new cancer biomarkers. We...antibodies to be used as a powerful reagent in the laboratory.   The DSHB focuses on monoclonal antibodies,...Hybridoma Bank (DSHB) since 1996.       More Blog Posts about Scientific Sharing: The 10 Most Distributed Plasmid...

  6. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...lentiviral packaging as a single payload (Figure 4). Figure 4: ONE-GO biosensors measure the formation...synaptic events By Brian O’Neill The Lin Lab and collaborators recently created ASAP5, a faster and more responsive...

  7. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927. https://doi.org/10.1016/J.CELL.2013.07.018... 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J., & Liu, D. R. (2023). Prime editing for... al., 2023). We trained our models in close collaboration with the Krauthammer lab (University of Zurich...

  8. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...:3499-3510. doi: 10.1002/bit.27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley...absorption coefficient of a protein. Protein Sci. 1995 Nov;4(11):2411-23. doi: 10.1002/pro.5560041120. PMID: 8563639...validated, it’s ready for use! You can learn more about common antibody applications in our Antibodies 101...

  9. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...buffer. Additionally, protein lysis should be done at 4 °C or on ice, whichever is more practical. If your...protein-based blockers degrade fairly quickly, even at 4 °C, so when in doubt, make up a fresh batch.  Antibodies...temperature and time. Though the most common conditions are 4 °C overnight or room temperature for 1–2 hours, there...protein/sample loaded Incubate primary antibody at 4 °C overnight Incomplete washing Ensuring wash...re in the right place! In this post, we’ll talk about western blot optimization and troubleshooting.   ...can be informative, so spend some time thinking about which type of gradient can help answer your particular...overexpression. Before starting a western blot, think about how you’ll interpret the data and what level of ...

  10. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...tech transfer and the adoption of the UBMTA Chapter 4 - Developing Addgene as a modern e-commerce company...repository’s future. If you’ve ever been curious about what it takes to start your own nonprofit, this ...Harvard University, where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge...

  11. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...of 39 Scientific Impact Measures" (2009) PLOS One 4(6): e6022. doi: 10.1371/journal.pone.0006022 Aragon...Of course, all of you toiling away in laboratories this holiday season want the work you are doing to... has to do with online accessibility. It is now about as easy to find a paper in a lesser-known journal...

  12. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...in labcoats (@ladiesinlabcoats) on Dec 19, 2018 at 4:36am PST Buddy the Elf from Matthew Sikora’s lab...more (stay tuned for a few blog posts coming up about sustainability in the lab!). The @ladiesinlabcoats...

  13. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...the sequence isn’t in place yet.     Figure 4: S. pyogenes Cas9 in the apo state (PDB ID 4CMP, top...641–656. https://doi.org/10.1038/s41579-022-00739-4 References Bravo, J. P. K., Liu, M.-S., Hibshman, ...sequence. Nature Structural & Molecular Biology, 29(4), 395–402. https://doi.org/10.1038/s41594-022-00756...nascent heteroduplex and abolish cleavage (Pattanayak et al., 2013). As mentioned above, however, Cas9 sometimes..., 2022), indicating that there is more to learn about this process!      Figure 3: Crystal structure...rest of Cas9 and is highly flexible; it can rotate about 140 degrees between inactive and active conformations...conformational changes that make Cas9 such a valuable laboratory tool are still an area of active research. New...

  14. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...the contact to start a direct collaboration between researcher and expert; 4) Credit all contributions. ...our blog post about remote collaborations Read our Transferable Skills Guide article about external collaborations...needed. In the example above, for example, we fostered a formal scientific collaboration between two research...project. We think that these collaborations add enormous value. A discussion about a protocol can save weeks..., the researchers did not have access to a P3 laboratory (BSL-3), a high-security facility needed to perform...approach was useful in humans. Then, one of them heard about our online platform, called Crowdfight, and filed...just the right infrastructure and offered to collaborate with them. The partnership was fruitful and a...

  15. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ... importantly, it’ll help you define salary costs. 4. Ascribe a cost structure associated to these tools...others will help prove that you will be an asset. 4. Always ask, “Is there an alternative?” As scientists...a successful negotiation and collaboration. We’d love to hear more about your experiences and opinions...can get away with using computer modeling and collaborations rather than using animals, then that’s a cost...associated with old equipment, like service repairs, labour costs, and loss of time, can exceed the cost of...Everything is negotiable. Try asking for discounts, collaborations, access to internal award applications, etc...This is a non-profit organization. Therefore, collaboration and community is the ultimate value proposition...

  16. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...efficacy of your antibody. When compared, 4% PFA and 0.1% saponin; 4% PFA and 0.1% Triton X-100; and methanol...and KO cells were mixed in a 1:1 ratio and fixed in 4% PFA and permeabilized in 0.1% saponin. 400,000 cells...leukocytes. The file shared also contains information about the labeling pattern of several cell lines using...Laflamme, C., Virk, H., NeuroSGC/YCharOS/EDDU Collaborative Group, & ABIF Consortium. (2024). A guide to...

  17. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...million views from 119 different countries (Figure 4). Four of the five most popular videos are protocol...other videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel,...a less tangible form: knowledge and information about the materials in our repository. We started to increase...by making it easier to find materials and learn about them. As early as 2007, we began grouping similar...professors, stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to...seeing our resources shared via social media, on collaborators’ websites, or as a recommendation in a Reddit...updating and expanding our website, we’ve learned about the importance of smaller, systemic changes, like...

  18. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...and AAV1 are the most requested serotypes (Figure 4), our catalog includes vectors in a variety of serotypes... and the systemic capsid PHP.eB.   Figure 4: A) Cumulative AAV preps for the seven most popular...all, a delivery method. I would be remiss to talk about them without looking at what they’re delivering .... But in the years since, we’ve learned so much about what it takes to make these viral vectors not only...., 2016), video, educational resources, and collaborations, and will continue to do so, in the hopes that...leave a comment; or take our AAV survey. Learn more about GCaMPs and other tools developed through the GENIE...

  19. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...conservative estimate. As a graduate student, I drank 3-4 cups of coffee a day, and I had colleagues who were...learn about all the amazing research being done with plasmids. I heard an impressive talk about the use...pulls off such an inspiring, thought-provoking, collaborative event – all while keeping the party going! At...scientists we've spoken with are excited and passionate about making such tools open-access. Though it is time...

  20. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...PMID: 19396156. PubMed Central PMCID: PMC3655711. 4. Gronostajski, R M, and P D Sadowski. 1985. “The FLP...and Removed at Persistent Sites In Vivo.” Neuron 89(4): 756–69. PubMed PMID: 26853302. PubMed Central PMCID... on the Addgene Blog Learn More about FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs...expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and...
Showing: 121 - 140 of 340 results