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We narrowed to 168 results for: acr.2

Showing: 121 - 140 of 168 results

  1. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...still depolarize neurons at lower MOIs. Figure 2: ChRgers are delivered by intravenous injection into... to perform in vivo optogenetic experiments. Intracranial injections of AAV are commonly used to deliver...neurons and mouse brain slices collected following intracranial injection of an AAV expressing a ChRger. In ...nervous system which eliminates the need for intracranial injections. When packaged in PHP.eB, ChRgers...

  2. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...divided by the off-target activity. Figure 2: Specificity is the measure of off-target activity...off-target activity, relative to eSpCas9 and SpCas9-HF across a number of different sites. Using a combined random...high degree of both specificity and sensitivity across a large number of loci and gRNA lengths. Recent...

  3. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...available in the adenovirus section below!   Fig. 2 - Viral vector components of AAV Adenovirus Adenoviruses...protocols! In reality, there are many shared components across viruses (AAV, lentivirus, etc.) and their functions... The components of these vectors are also split across several plasmids (often three), for safety purposes...

  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ... the Cas machinery where to cut.      Fig. 2: Heatmap indicating loci where SNPs reside on sgRNA...the breakdown of ancestry associated dependencies across ancestry groups (right).    Seeking an explanation...not represent the diversity of genetic variation across the human population. Rather, it primarily reflects...which SNPs map to thetargeting sequences of guides across 8 CRISPR libraries. Samples are divided into those...in CRISPR-based experiments with varying burden across ancestries. Sean A Misek, Aaron Fultineer, Jeremie...

  5. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...contain the expected plasmid features.  Figure 2. Addgene’s basic QC process. Created with BioRender.com... us to have a high standard of sequence quality across all plasmids in our collection. This standardization...we maintain a high standard of sequence accuracy across all of our plasmids and provide transparency in...sequence-verified constructs to share with scientists across the globe. This post was written by Alyssa Shepard...

  6. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ... tabular HTML formatted output. Surprisingly only 2 tools provide FASTA output. A few notable tools allow...Nature biotechnology(2014). PubMed PMID: 24880488. 2. Hsu, Patrick D., et al. "DNA targeting specificity...determine the value of every sweet or toy strewn across the floor. In the final wake it all settles down...functional. It is also the only tool I have come across to offer classes of parameters based on the type...

  7. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...(S) labeling of the cell population. Figure 2: Dense (c), intermediate (d), and sparse (e) labeling...SPARC technology is versatile and can be applied across cell types and organisms by replacing the transcription...

  8. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine sensor In 2018, Lin Tian’s ...liquid, which causes the passive diffusion of DA across the semipermeable membrane from the extracellular...

  9. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...development and therapy. Nat Rev Drug Discov (2017) 16(2):89-100. DOI: 10.1038/nrd.2016.238 Additional resources...for use in primary cells, many tissue types, and across species, it enables researchers to select and generate...

  10. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...beginning Let’s take a quick flash back to 2004. “Shrek 2” was the most popular movie in theaters, the Razr ...support sharing of CRISPR constructs and information across the globe.  “Read the Addgene guide: https://www.addgene.org...comprised of 1302 plasmids, was shared with researchers across the globe. As the pandemic slowed, Addgene began...

  11. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ... PMID: 25184501. PubMed Central PMCID: MC4262738. 2. Doench, John G., et al. "Optimized sgRNA design to... method (Tanenbaum et al., 2014). The sgRNA or tracrRNA sequences can also be modified to include RNA ...transcription factors. The Root and Doench labs utilized tracrRNAs with two PP7 stem loops to recruit additional ...the targeted region (Sanson et al., 2018) . This tracrRNA was combined with the sgRNA expression vector ...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...products have distinct localizations. Figure 2: Split-wrmScarlet labeling proteins with different...-FT consistently predicted the cell cycle speed across these different cell types. In one example, H2B-FT...Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory...

  13. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...Hoogsten base pairs and wobble pairs.  Figure 2: (A) The codon wheel depicts the 64 codons that encodes...translational efficiency is correlated with codon bias across all endogenous genes in E. coli and S. cerevisiae...electronic dataset for codon-usage of 257,468 genes across 8,792 organisms (Nakamura et al., 2000).  What ...to identify the ideal codon for every amino acid across an entire polypeptide. Many researchers have created...

  14. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused...coordinates optical stimulation of its associated fibers across multiple channels and can also record tissue temperature...

  15. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...require when making such a decision remains spread across literature in publications that introduce these...

  16. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...capsids, when writing this post, we didn’t come across a broadly used working definition of systemic capsids...expression (EGFP, in the depositing manuscript) across the brain. AAV-BI-28 has been tested in C57BL/6J...brain cells, with a higher efficiency (82 — 85% across the brain in mice) and greater specificity than...effectively and predominantly transduces neurons across brain regions, with reduced off-target expression...

  17. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...overview of these new variants:   Figure 2: Inner mitochondrial membranes imaged with structured...X1.1 efficiently transduce brain endothelial cells across diverse mice strains and rats. Figure from Chen... et al. (2023). Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific...

  18. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...integrates into the trnS-leu2 locus.   Figure 2: Introducing Mobile-CRISPRi into bacteria. Top: A ...constitutely expressed RFP reporter, was variable across bacterial strains, ranging from an ~8 to 150-fold... reduction, with a median knockdown of ~40-fold across all species measured. Beyond understanding the ...

  19. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ... enrich the microvesicles containing AAVs (Figure 2). Since the development of the exo-AAV isolation protocol...secreted from producer cells also contain cellular macromolecules (e.g. proteins, nucleic acids) which possibly...

  20. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...of concept for further development. Figure 2: Mechanisms of deaminase-based adenine transversion...refresher if you ever lose track of which acronym is which. Acronym Full name Notes   Base transition ...close at hand? Once again, this post has a lot of acronyms, so we've included a glossary at the end of this...polymerase known to preferentially incorporate A bases across from abasic sites. This approach resulted in a ... case, encouraging it to incorporate a new base across from the abasic site before filling the empty space...
Showing: 121 - 140 of 168 results