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We narrowed to 512 results for: ato

Showing: 121 - 140 of 512 results

  1. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...characteristics that make them ideal for studying in a laboratory environment. We’ve covered five model organisms...have been specifically developed to thrive in laboratory settings. One of its most attractive features...characterized genome, and is easily maintained in a laboratory environment. What sets Arabidopsis apart from.... The Norway rat, from an obnoxious pest to a laboratory pet. ELife, 9. https://doi.org/10.7554/elife.50651...

  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch...SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang...

  3. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...transcriptional repressor), and dCas9-VP64 (transcriptional activator)-containing plasmids. Each destination vector ...one. This approach is especially useful for combinatorial analyses. For example, if you were to develop...A versatile modular vector system for rapid combinatorial mammalian genetics.” Journal of Clinical Investigation...Cress, Brady F., et al. “CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9...

  4. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...pAAV-Ef1a-fDIO-ChrimsonR-tdTomato AAV9 Controls Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9...multiple serotypes pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes...

  5. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...ways scientists have adapted them for use in the laboratory. The discovery of GFP The story of how fluorescent...sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous organisms...studying it grew. In particular, studies in the laboratory of UCSD biochemist Roger Tsien resulted in much...

  7. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...transduction of Cas9-expressing (Rosa26-Cas9) hematopoietic stem cells with a lentiviral vector containing...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential ...

  8. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the cell wall of green...forcing it out of hiding to become exposed to predators. Hungry birds spots the “caterpillar” and then... "Back from the dead; the curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus." ...

  9. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ... interpret signals from these reporters. Lack anatomical context/spatial localization: In vivo imaging...years to clinically treat enteric infection and inflammatory bowel conditions. In these experiments, arg1...abdomen. arg1 signal, however, can be overlaid onto anatomical ultrasound imaging to show where the bacteria...

  10. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...scientists. Over the course of the class, the moderator discussed individual sections of a paper, the ...quizzes and discussion on that week’s topic by the moderators. The assignments, which consisted of reading ...course was breaking up into focus groups with a facilitator. In the focus groups, we were required to critique...

  11. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...passion, and dedication to sharing epitomized by the creators of these plasmids with the first annual Blue Flame...has “literally enabled sharing with 100's of investigators worldwide. Now everything from every paper is...Flame awardee Sophien Kamoun of the Sainsbury Laboratory in Norwich, UK: “We’re very pleased to distribute...

  12. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ...department.” Caitlin does this by acting as a facilitator between her team, the sales department, and operations...business.” Caitlin’s advice for budding science communicators: “My recommendation would be to take any opportunity...education tools? Check out this Addgene podcast with educator and science rap mastermind Tom McFadden Get tips...

  13. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...plasmid. Parts shown in grey are optional cis-regulatory elements.   Key elements of an AAV plasmid...plasmid harbors non-coding sequences. Other cis-regulatory elements Next let’s talk about some of the elements...Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary structure when...

  14. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...Willoughby   Using RUBY as a selection marker The creators of RUBY highlight its use in crop plants under... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...promoter with self-cleaving 2A site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al....

  15. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...Neurobiology has been working on genetically encoded indicators of calcium and other small molecules since the...imaging for beginners Griesbeck says his calcium indicators can be used in mouse, Drosophila, C. elegans,...his team is now applying it to improve upon an indicator they developed and published in 2010 for visualizing...

  16. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...15-30 minute break. The method is named for the tomato or “Pomodoro” kitchen timer that the technique’...student.   The Pomodoro timer is literally a tomato! Image from Gerlos.   Tools for checking grammar...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...

  17. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...to create gene deletions of important unknown regulatory regions in Pseudomonas and Klebsiella spp. The...Ibadan, Nigeriaunder supervision of Dr. Akindele Oluwatosin Adeyi, University of Ibadan, Nigeria Gloria Otito...Candidate working under the supervision of Akindele Oluwatosin Adeyi at the University of Ibadan. She works ...

  18. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...culture and include a counter, lab timer, dilution calculator, and a couple of other nice features. This app...sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on ...apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega Material...

  19. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...histidine kinase that phosphorylates a response regulator (RR). TCS-induced responses are varied; one outcome...conditions, the chromophore activates the response regulator via phosphorylation, promoting transcription of...spread across three plasmids, with the response regulator encoded in the genome. To make the system less...

  20. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...identified other advantages, including Cas13d-specific regulatory proteins and high targeting efficiency, both ...WYL domain-containing proteins may be broader regulators of Cas13d activity. This property makes WYL proteins...REPAIR Get tips for using Cas9 Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources...
Showing: 121 - 140 of 512 results