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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 319 results for: bre

Showing: 121 - 140 of 319 results

  1. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...gRNA Figure 1: Validation of double strand break (DSB) mediated homology dependent repair by enhanced...better that gRNA is at causing a double stranded break at the target site, allowing the two halves of EGFP...

  2. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...offense to BRET, CRET and other RET techniques which have never outclassed the Master. Note: BRET= Bioluminescence...

  4. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...frameshift mutations through aberrant double strand break repair, it has proven more challenging to develop...editing via the introduction of a double strand break by using a targeted nuclease. Asuri et al, for example...

  5. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ... (HDR) of a CRISPR/Cas9 induced double-stranded break. This site-directed genome editing requires a template...researcher at Cornell University.       References 1. Breslauer, Kenneth J., et al. "Predicting DNA duplex stability...

  6. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...to specific loci without inducing double strand breaks. Below you'll find some of the CRISPR-based epigenetic...programmable and targeted de novo DNA methylation in breast cancer. Oncogene 34:5427–5435 . https://doi.org...

  7. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...Part 1: Genetically Engineered Mice and Part 2: Breeding and Crossing Mice. My first CRISPR mouse experiment...endonuclease that creates the DNA double-strand break (DSB) at the target site, and a donor oligonucleotide...

  8. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...standard DNA plasmid transfection, a double-stranded break may occur in your cargo. Additionally, with piggyBac...as CRISPR/Cas9) to create a double-stranded DNA break. After DNA cleavage, the piggyBac™ donor is used...

  10. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...allowing for easy multiplexing. The double-stranded breaks caused by Cas12 create short 3’ overhangs, which...hypothesis, and even the time and money available. The breadth and diversity of CRISPR systems will no doubt keep...

  12. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...Nihongaki, et al., 2015) resulting in a double strand break (DSB) that can be repaired by either non-homologous...References Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner K, Fakhiri ...

  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...targeted known residues on the outer surface to break dimerization interfaces (affecting many of the RFPs...of cytosine to thymine without a double stranded break. However, the method was initially limited by off-target...

  14. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...Single Strand Annealing (SSA) in the event of a DNA break. Next, the gRNA being tested is expressed along ... simplifying experiments when additional cross-breeding steps are undesired or infeasible. Finally, an...

  15. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ... improve and redesign our pipeline. In it, they break down the unique challenges of our platform and how...
Showing: 121 - 140 of 319 results