...certainly taken notice! Our newest page in the Transcriptional Regulation category already has five blue flame...with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated gRNAs for Regulating ...

...better. More transcription factor binding sites in a promoter lead to higher transcriptional activation....

...ready-to-use viral preps   Improved cell-free transcription/translation system to examine biosynthetic pathways...pathways  By Gabrielle Clouse Recently, cell-free transcription/translation systems (also referred to as TX-...

...WPRE The Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary... of RNA and RNA translation, and promotes RNA transcript longevity. The polyA is located at the end of...

...programmed regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests...each day brings in new and surprisingly diverse manuscripts. I work with people, nurturing networks, as much...

... categorized either based on the section of a manuscript they might be found, i.e. introduction, methods...the Addgene blog: Tips for writing scientific manuscripts: literature searching, reading, and organizing...

... knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm presents an attractive...! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol...

...are also thought to play a key role in the transcriptional regulation of many genes. A number of groups...thus far for these latter experiments, such as transcription factors tethered to dCas9, require long time...

...luciferase, usually serves as an indicator of transcription within cells, where detection of the reporter...transfected into the desired cells. A hybrid mRNA transcript containing luciferase and the gene of interest...

...Chrimson Through de novo sequencing of 127 algal transcriptomes, as well as further optimization through engineering...Mar 11;(3):338-46. For more information and descriptions of various optogenetics plasmid tools, visit...assembled into a Level 1 vector creating a single transcriptional unit (Ex: a promoter, 5’UTR, coding region,...

...Pol, the last component, contains the reverse transcriptase and integrase components to convert the cargo...the primary viral polymerase. E4/E1A activate transcription of other relevant viral genes (including the...

...single-stranded RNA viruses that use reverse transcriptase to synthesize complementary DNA copies of their... such as a heterologous envelope or a post-transcriptional regulatory element can be used for either type...

.... elegans. These dsRNAs target specific mRNA transcripts for degradation. Despite how easy these experiments...target gene between two inverted T7 promoters. Transcription from each promoter progresses past the specific...

...red. The variation in color depends on how transcriptionally active the genomic locus of integration is...Using a Drosophila cell line, I’ve studied a transcription factor important for T cell differentiation ...

...transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9 Nissim et alTsai...transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9   Xie et al  ...

...IPTG is also included in the medium to ensure transcription of the lac operon.   Tips for blue-white screening...α-complementation cloning MCS): pGEM-T, pUC18 and pUC19, and pBluescript are a few common vectors. Blue-white screening...

...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined...

...deletions and introduce epitope tags and RNA transcription start sites. The lab further demonstrated that...comprises an active Cas9 fused to the reverse transcriptase (RT), which induces two double-strand breaks...

...expression of other genetic elements including transcription factors (Cheng et al., 2014) and microRNAs (.... Konermann, Silvana, et al. "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex...

...of citations per year and the number of other manuscripts that heavily cite the paper are displayed. You...the context of these citations. The number of manuscripts that cite the background, methods, or results...