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We narrowed to 372 results for: ena

Showing: 121 - 140 of 372 results

  2. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...after Nanoblade treatment as measured by their maintenance of pluripotency marker expression and response...Nanoblades targeting the hydroxyphenylpyruvate dioxygenase (HPD) gene, all nine treated mice had ~10% editing...

  3. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...Institute (Harvard University), we developed two new enabling technologies for the field of synthetic biology... proof-of-concept work was exciting because it enabled gene circuit-based sensors to be used outside of...

  6. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...optogenetic or fluorescent tool with magnets would enable new research and applications that don’t yet exist...Gaykema, R. P., Spano, A. J., Beenhakker, M. P., Kucenas, S., Patel, M. K., Deppmann, C. D., & Güler, A....

  7. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...that nearly all of the cell lines we tested were amenable to cloning in this manner, we once had difficulty...have prepared two 48-well plates of clones) which enables investigators to identify specific clones with ...

  8. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...This approach can augment viral surveillance by enabling rapid assessment of antigenic drift.    Starr...rate-limiting enzyme in this pathway (myo-inositol oxygenase), providing enzyme protection while still allowing...

  9. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...” transistor-like digital genetic switches that enable cellular computing. These logic gates built from...available via Addgene and the BPA Plasmid Collection - enable? Endy: The BIOFAB collection is the beginnings ...

  10. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...complementing mutant phenotypes. Arabidopsis is amenable to the floral drip or dip transformation method...at Stanford University. She studies cell fate maintenance in the stomatal lineage and has made countless...

  12. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...Cas13, REPAIR is the first CRISPR-based system to enable precise RNA editing. The addition of RESCUE further.... "Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature...

  13. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...bacteria and denature the DNA and proteins in solution. The pH is then lowered using a renaturing solution...

  14. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...Payen E, Negre O, Wang G, Hehir K, Fusil F, Down J, Denaro M, Brady T, Westerman K, Cavallesco R, Gillet-Legrand... Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Read about Genome-Wide...

  15. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... which may also induce cell death. CRISPR also enables increased specificity - rather than targeting a...antibiotics, but the field is currently undergoing a renaissance. Yosef et al. designed a two-phage CRISPR system...

  16. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ... of such engineered DNA-binding molecules even enables us to skip the step of knocking-in. In 2013, we...dCas9 and guide RNA. The retroviral enChIP system enables us to express the CRISPR complex much easier in...

  18. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...retains important functional properties, and is amenable to a wide range of biophysical assays (Kroupova...low-expression proteins of interest) and mScarlet-I3 for scenarios requiring rapid maturation and prolonged imaging...

  19. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...very flexible cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously.... "Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature...

  20. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ..., … Li, D. (2024). Adenine transversion editors enable precise, efficient A•T-to-C•G base editing in mammalian...optimization of a uracil-N-glycosylase variant enables programmable T-to-G and T-to-C base editing. Molecular...
Showing: 121 - 140 of 372 results