We narrowed to 147 results for: lenti
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TypeBlog PostPublishedApril 13, 2023, 1:15 p.m....is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ...
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Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog PostPublishedOct. 20, 2022, 1:15 p.m....Request ready-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog PostPublishedSept. 8, 2020, 2 p.m....in women with TS (Trolle et al., 2016). Using lentiviral vectors from Addgene, the lab will express these... -
Viral Vectors 101: An Introduction to AAV
TypeBlog PostPublishedMay 30, 2023, 1:15 p.m....? Check out Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog PostPublishedNov. 7, 2017, 1:59 p.m....technologies have been investigated including lentiviruses, adenovirus, and inorganic delivery tools such... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog PostPublishedAug. 11, 2016, 2:23 p.m....out Our Primer for Beginners! Learn All about Lentiviral Vectors Additional Resources on the Addgene.... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m.... Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m.... for Your Experiments Get Ready-to-Use CRISPR Lentivirus ... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog PostPublishedOct. 30, 2018, 1:04 p.m....iPSCs from dermal fibroblasts via retroviral and lentiviral infections respectively [9, 10]. These stellar... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....proportions from a vector backbone that allows lentiviral packaging as a single payload (Figure 4). ... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....production process. Helper-free production Unlike lentivirus, AAV does not possess the ability to replicate... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m....difficult to work with. For example, a library in a lentiviral backbone might be more prone to recombination... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m.....org Find Ready-to-Use AAV Find Ready-to-Use Lentivirus Browse Viral Plasmids ... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....theoretically possible, but likely more challenging, with lentiviral expression. For more detail on how to design ... -
Lab to Office Culture Shock
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....focusing on my technical skills with cloning and lentivirus production, I didn’t really start to recognize... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....2016). Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m.... as RNAP II promoters is commonly employed in lentiviral and retroviral constructs and we will discuss... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog PostPublishedSept. 1, 2020, 1:15 p.m.....7kb, or roughly half the packaging limits of lentiviral and adenoviral vectors. While many transgene ...