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  1. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...

  2. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...materials to be deposited into repositories, and financial support to maintain repositories’ capacity for...-5247(20)30004-5 McCray PB Jr, Pewe L, Wohlford-Lenane C, Hickey M, Manzel L, Shi L, Netland J, Jia HP...

  3. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...your DNA prep and whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally... run, DNA fragments move through a micro- or nanofluidic channel and the sample is separated by electrophoresis...

  4. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...trait: Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology...

  5. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...their first protocol is available now. It is for recombinant expression of phosphoserine-including proteins...Site-specific Incorporation of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https:/...

  6. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...project involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for...export and shipping on dry ice. The Business and Finance Teams continue to facilitate the formation of partnerships...

  7. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of ..., but it’s also found in tissues. IgG is the predominant isotype during a secondary immune response, which...

  8. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ... we could use the same principles to provide recombinant antibodies, another plasmid-based product that... In March 2022, we launched our ready-to-use recombinant antibodies service, part of the Neuroscience ...

  9. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...not a “Fab” fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide...conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have the highest tissue penetration...

  10. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...The introduction of antibody fragments, such as nanobodies, has introduced new options for increasing signal... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene  ...

  12. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...surface and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells interact, ...fluorophore/antibody pairs. Because the anti-GFP nanobody cross-reacts with BFP, sender cells expressing...

  13. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ... Part 1 of this series). In any case, Cre is a dominant phenotype, while you’ll want the floxed gene to...836. PubMed PMID: 29768166. Zickler, Denise, and Nancy Kleckner. "Recombination, pairing, and synapsis ...

  14. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...includes I152L in N-terminal fragment An enhanced recombinant amino-terminal acetylation system and novel in...Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other split protein tools: Split-TurboID...

  15. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...setting. Molecular diagnostics for the Zika virus predominantly look at viral load in patient blood and urine... carries out research in synthetic biology, nanomaterials science, and self-assembly for applications ...

  16. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...PMC84922. 5. Ugai, Hideyo, et al. "Stability of a recombinant adenoviral vector: optimization of conditions...Heidelberg, 2001. 291-306. PubMed PMID: 10634178. 8. Murnane, John P., Michael J. Yezzi, and Barbara R. Young...

  18. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...Figure 1). Through this method, we found key determinants of prime editing efficiency such as the length...efficiencies using sequence features and DNA repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org...

  20. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ... concentrations of iodixanol to separate out contaminants from an impure AAV preparation. 15%, 25%, 40%...R.A., & Samulski, R.J. (2007). Production of recombinant adeno-associated viral vectors for in vitro and...
Showing: 121 - 140 of 317 results