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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 322 results for: nans

Showing: 121 - 140 of 322 results

  1. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and its derivatives...multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or bio-layer interferometry...

  2. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...how punctuation can be wildly funny as well as financially critical. Those who work on the publishing side...the right audiences and how open access affects financial models in information publication. Take a look...

  3. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...fragment, single-chain variable fragment (scFv), or a nanobody is one potential remedy for this problem within... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...

  4. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...materials to be deposited into repositories, and financial support to maintain repositories’ capacity for...-5247(20)30004-5 McCray PB Jr, Pewe L, Wohlford-Lenane C, Hickey M, Manzel L, Shi L, Netland J, Jia HP...

  5. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...your DNA prep and whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally... run, DNA fragments move through a micro- or nanofluidic channel and the sample is separated by electrophoresis...

  6. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...

  8. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...trait: Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology...

  9. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...project involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for...export and shipping on dry ice. The Business and Finance Teams continue to facilitate the formation of partnerships...

  10. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of ..., but it’s also found in tissues. IgG is the predominant isotype during a secondary immune response, which...

  11. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...their first protocol is available now. It is for recombinant expression of phosphoserine-including proteins...Site-specific Incorporation of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https:/...

  12. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ... we could use the same principles to provide recombinant antibodies, another plasmid-based product that... In March 2022, we launched our ready-to-use recombinant antibodies service, part of the Neuroscience ...

  13. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...not a “Fab” fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide...conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have the highest tissue penetration...

  14. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...The introduction of antibody fragments, such as nanobodies, has introduced new options for increasing signal... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene  ...

  15. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ... Part 1 of this series). In any case, Cre is a dominant phenotype, while you’ll want the floxed gene to...836. PubMed PMID: 29768166. Zickler, Denise, and Nancy Kleckner. "Recombination, pairing, and synapsis ...

  16. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...includes I152L in N-terminal fragment An enhanced recombinant amino-terminal acetylation system and novel in...Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other split protein tools: Split-TurboID...

  18. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...surface and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells interact, ...fluorophore/antibody pairs. Because the anti-GFP nanobody cross-reacts with BFP, sender cells expressing...

  19. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...setting. Molecular diagnostics for the Zika virus predominantly look at viral load in patient blood and urine... carries out research in synthetic biology, nanomaterials science, and self-assembly for applications ...

  20. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...PMC84922. 5. Ugai, Hideyo, et al. "Stability of a recombinant adenoviral vector: optimization of conditions...Heidelberg, 2001. 291-306. PubMed PMID: 10634178. 8. Murnane, John P., Michael J. Yezzi, and Barbara R. Young...
Showing: 121 - 140 of 322 results