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We narrowed to 363 results for: nsf

Showing: 121 - 140 of 363 results

  1. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...enables selection of cells with the plasmid post transfection. The 2A "self cleaving" peptide causes the ribosome...backbone. This particular backbone can be used to transfect a variety of mammalian cells and can be modified...can be used to test for SpCas9 expression post transfection by western blot. Overall, this is a versatile...

  2. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...system which has been designed to perform gene transfer in vertebrates. The system is comprised of 2 components...the lab showed that efficiencies of stable gene transfer using SB100x were comparable to stable transduction... Beauty transposase enables robust stable gene transfer in vertebrates. Nat Genet. 2009 Jun;41(6):753-...

  3. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...mammalian selection cassette to screen for positive transfected cells. The addition of an in-frame fluorescent...you can get away with generating transiently transfected cells, you will not need a mammalian selection...create a new strain with a disrupted gene by transforming wild-type yeast with an integrating vector expressing...

  4. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...plasmids from the same group should not be co-transformed, so if you need two plasmids for an experiment... same incompatibility group should not be co-transformed. +Actual copy number varies. See below for ...stocks, agar stabs, or liquid cultures. Freshly transformed cells also give a higher plasmid yield that colonies...

  5. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...Resistance gene and Selection Markers) and one “Transfer Plasmid” game board. Once they agreed on each ...had 35 minutes to develop a strategy and build a transfer plasmid that could be used to genetically manipulate... this way, the students also had to practice transferable skills like team building, collaborative critical...

  6. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ..., you’re ready to edit. These cells are then transfected with several different constructs for the reporter... gRNAs by simply changing the gRNA used when transfecting your cells.  A plasmid that contains Cas9. The...plasmid.  With this set up, all successfully transfected cells will express RFP while cells containing...

  7. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...pSpCas9(BB)). The 2A-GFP allows for screening of transfected cells. Find pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR...lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver... David Root lab is a 3rd generation lentiviral transfer plasmid for cloning and expressing new shRNA sequences...

  8. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...DNA molecule as the gene that they package. In a transfer plasmid, the transgene is flanked by two ITRs,...and rAAV vectors, modifications can affect gene transfer efficiency and transgene expression (Zhou et al...of ITR and capsid serotypes vary in their gene transfer efficiency. Our practical knowledge of ITRs is...

  9. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...the delivered cargo (often a gene) is called the transfer plasmid while the other plasmids with packaging... or something similar, and is contained on the transfer plasmid. If expression is the goal, a promoter...delivered and expressed in the host cell from the transfer plasmid. Viral packaging and entry The viral packaging...

  10. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...DNA. The other component of these systems, methyltransferases, methylate DNA at particular sequences to...restriction modification systems containing methyltransferases that add methyl groups to specific DNA sequences...plasmids with no methylation (there are no methyltransferases in the PCR reaction). The template plasmid...

  11. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...produces pigment from x-gal (included in the transformation plate medium), turning the bacterial colony...for blue-white screening Use a good control: Transform the backbone plasmid without insert. All colonies...components, and are rescued by genes supplied on the transformed plasmid. Such plasmids may contain genes that...

  12. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ... still in its infancy. A long timeline from transformation to testing and a lack of well-characterized...s made even more difficult by the fact that transformation methods have also been patented. In order to.... We will also be working with open source, transformation technologies like those pioneered by Ewen Mullins...

  13. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...base editor is split into two plasmids and co-transfected into cells for reconstitution. Image from Levy...promoters, CRISPRi parts, selectable markers, transformation acceptor vectors, and sequences for genomic...plasmids using the Cyanogate Kit and subsequent transformation into cyanobacteria. Image from Vasudevan et...

  14. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...PPI) studies in plants that rely on transient transfection systems using Agrobacterium (such as Nicotiana...al., 2016)). During Agrobacterium-mediated DNA transfer, each GOI is usually expressed from a different...plasmids to study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or ratiometric Bimolecular...

  15. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...microenvironment. This results in increased expression of transforming growth factor-β signaling, possibly inducing... (micro RNA) and other molecules that can be transferred to other cells, inducing epigenetic changes in.... "Intercellular nanovesicle‐mediated microRNA transfer: A mechanism of environmental modulation of hepatocellular...

  16. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...separate envelope and packaging plasmids as well as transfer plasmids. This concept ensures safe handling and...Gamma-retroviral vectors Popular gamma-Retroviral Transfer Vectors   Expressing your gene of interest pMKO...interest. Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your gene of interest pLenti-puro...

  17. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ... the E1 gene from the packaging cell line is transferred into the AdV by recombination. It is therefore...correct recombinant pAdV plasmid is identified, re-transform into a bacterial strain not prone to recombination... adenoviral stock (rAdV-S). HEK293 cells are transfected with the AdV plasmid construct from step #1 and...

  18. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...looks like the genetic cargo in one plasmid (the transfer plasmid), the envelope (Env) gene on a separate... plasmid). Viral production then proceeds by transfection of all three plasmids into a producer cell line...Env, Gag, and Pol genes are not encoded in the transfer plasmid, the component that physically integrates...

  19. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...blot Run your gel and membrane transfer as normal. Immediately after transfer, dry the membrane to maximize...from having to run a whole new gel and membrane transfer. Let’s get started! Before you begin In the best...
Showing: 121 - 140 of 363 results