...         Adipocytes [28] Keratinocytes [29] Photoreceptors [30] Otic hair cells [31] Neurons [32, 33...Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem... Deaf Patient With MYO7A Mutation Results in Morphologic and Functional Recovery of the Derived Hair Cell-Like...

...the RNAs from cellular defenses against ssRNA: phosphatase treatment, substituting cytidine with 5-methylcytidine...pluripotency, but they also take less time to complete. Morphology is a simple and fast to evaluate. iPSCs should...little cytoplasm. Positive staining for alkaline phosphatase, expression of embryonic proteins, and promoter...

...festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the holidays...Festivus— you name it, we want to see it.      All photos tagged with #DeckTheLab throughout the month of...

...party, we shared memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured...over the years, including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable...

...of application and aid in visualization of the morphology of individual neurons, including dendritic and...vivo, microinjection, pressure injection or iontophoresis (applying small electric currents via inserted... project but neurons were traced with AAV2. Two-Photon imaging was used to generate a 3D finemap of neurons...

...to isolate two proteins from the jellyfish’s photoorgans; the first, which he called aequorin, gives off...hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. and Katushka/mKate...

...and provides a brief rundown of Sean’s top five smartphone apps you can use in the lab. Whether you’re tired...Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the...

...computer or smartphone. Evernote allows you to create notes directly from webpage clippings, photographs, and...

...of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (2013) PLoS ...requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or multi-color...

...a hassle. You finally make it through a 20-step phone tree, only to be left on hold for an hour. When ...the chance to stop what you’re doing and make a phone call or write out an email. You need help right ...

...lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the potential...

... Over 300 empty backbones with 100+ distinct fluorophores to choose from in the green, red, yellow, cyan... (e.g., apple, papaya, and tomato) and the photoactivatable fluors (e.g. Phamret and Dendra). It’s just...

...the overlap between groups.  They found that photostimulating the Kolmer-Agduhr cells was sufficient to ...Kolmer-Agduhr cells on one side of the body required photostimulation in order to drive the full symmetrical response...

...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...

...developed in vivo bacterial reporters that use fluorophores and luciferases to track bacteria in real time...vesicles are primarily used by water-dwelling photosynthetic bacteria and archaea to regulate their buoyancy...

... to students, like how to pour a gel [for] electrophoresis or doing a dissection video. I did that in ...themselves. “You open up the box and there’s a gel electrophoresis tray in there. Or the dissection specimens....

...FRET), in which a donor fluorophore is used to excite an acceptor fluorophore. The difference for BRET...

...affordable biosensors that detect herbicides, such as glyphosate, in water. He aims to leverage a glycine oxidase...oxidase reaction to indirectly detect glyphosate via H2O2, one of the reaction’s metabolites. One useful...

... Figure 1: An adult plant expressing RUBY. Photos by Andrew Willoughby   Using RUBY as a selection...green tissues is slightly darker or slightly brown. Photos by Andrew Willoughby The ease of observing ...

...element (tyrosine sulfation consensus sequence, fluorophore, or APEX2), allowing for quantitative analysis...fused with GFP, YFP, mCerulean, or other related fluorophores. Find all of the nanobody toolkit plasmids here...