We narrowed to 326 results for: pho

...preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver staining or SYPRO...purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are...transfection. PCR products are analyzed by gel electrophoresis to ensure the correct banding pattern and sizes...

...multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq ...not always achievable. Proteins which contain hydrophobic regions or multiple disulfide bonds may aggregate...

...General expression mRNA Mammalian promoter from phosphoglycerate kinase gene.  Constitutive  Widespread expression...yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong, also ...

...protein characteristics such as the brightness, photostability, maturation time, and acid sensitivity of various...sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated...PMC490022. Shaner, Nathan C., et al. "Improving the photostability of bright monomeric orange and red fluorescent...

...Stephanie Hays, a scientist with a passion for photosynthetic communities, microbial interactions, and science...Institutes of Health endanger scientists’ ability to uphold their side of the bargain and do the research required...University. She is a scientist with a passion for photosynthetic communities, microbial interactions, and science...

...after I’ve left the room. Microphone: Lastly, I needed to figure out a microphone situation. Generally, I...rules) to some volunteers, who filmed with their phones. Test: I loved the end result, but it was clear...

...visual reporter of RNAi, phenotypes, such as morphological parameters, can be easily scored. However, determining... a targeting sequence for the adenine phosphoribosyltransferase (APT) gene, which is conserved in many...

...         Adipocytes [28] Keratinocytes [29] Photoreceptors [30] Otic hair cells [31] Neurons [32, 33...Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem... Deaf Patient With MYO7A Mutation Results in Morphologic and Functional Recovery of the Derived Hair Cell-Like...

...the RNAs from cellular defenses against ssRNA: phosphatase treatment, substituting cytidine with 5-methylcytidine...pluripotency, but they also take less time to complete. Morphology is a simple and fast to evaluate. iPSCs should...little cytoplasm. Positive staining for alkaline phosphatase, expression of embryonic proteins, and promoter...

...festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the holidays...Festivus— you name it, we want to see it.      All photos tagged with #DeckTheLab throughout the month of...

...party, we shared memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured...over the years, including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable...

...of application and aid in visualization of the morphology of individual neurons, including dendritic and...vivo, microinjection, pressure injection or iontophoresis (applying small electric currents via inserted... project but neurons were traced with AAV2. Two-Photon imaging was used to generate a 3D finemap of neurons...

...to isolate two proteins from the jellyfish’s photoorgans; the first, which he called aequorin, gives off...hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. and Katushka/mKate...

...and provides a brief rundown of Sean’s top five smartphone apps you can use in the lab. Whether you’re tired...Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the...

...computer or smartphone. Evernote allows you to create notes directly from webpage clippings, photographs, and...

...of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (2013) PLoS ...requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or multi-color...

...a hassle. You finally make it through a 20-step phone tree, only to be left on hold for an hour. When ...the chance to stop what you’re doing and make a phone call or write out an email. You need help right ...

...lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the potential...

... Over 300 empty backbones with 100+ distinct fluorophores to choose from in the green, red, yellow, cyan... (e.g., apple, papaya, and tomato) and the photoactivatable fluors (e.g. Phamret and Dendra). It’s just...

...the overlap between groups.  They found that photostimulating the Kolmer-Agduhr cells was sufficient to ...Kolmer-Agduhr cells on one side of the body required photostimulation in order to drive the full symmetrical response...