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We narrowed to 317 results for: pho;

Showing: 121 - 140 of 317 results

  1. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...visual reporter of RNAi, phenotypes, such as morphological parameters, can be easily scored. However, determining... a targeting sequence for the adenine phosphoribosyltransferase (APT) gene, which is conserved in many...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...the RNAs from cellular defenses against ssRNA: phosphatase treatment, substituting cytidine with 5-methylcytidine...pluripotency, but they also take less time to complete. Morphology is a simple and fast to evaluate. iPSCs should...little cytoplasm. Positive staining for alkaline phosphatase, expression of embryonic proteins, and promoter...

  4. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

    Type
    Blog Post
    Published
    Nov. 30, 2015, 3:30 p.m.
    ...festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the holidays...Festivus— you name it, we want to see it.      All photos tagged with #DeckTheLab throughout the month of...

  5. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...party, we shared memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured...over the years, including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable...

  6. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...of application and aid in visualization of the morphology of individual neurons, including dendritic and...vivo, microinjection, pressure injection or iontophoresis (applying small electric currents via inserted... project but neurons were traced with AAV2. Two-Photon imaging was used to generate a 3D finemap of neurons...

  7. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...to isolate two proteins from the jellyfish’s photoorgans; the first, which he called aequorin, gives off...hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. and Katushka/mKate...

  8. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...and provides a brief rundown of Sean’s top five smartphone apps you can use in the lab. Whether you’re tired...Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the...

  10. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (2013) PLoS ...requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or multi-color...

  11. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...a hassle. You finally make it through a 20-step phone tree, only to be left on hold for an hour. When ...the chance to stop what you’re doing and make a phone call or write out an email. You need help right ...

  12. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the potential...

  14. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...

  15. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...developed in vivo bacterial reporters that use fluorophores and luciferases to track bacteria in real time...vesicles are primarily used by water-dwelling photosynthetic bacteria and archaea to regulate their buoyancy...

  20. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...use a red fluorophore-tagged anti-rat IgG1 secondary to detect Protein A and green fluorophore-tagged anti-mouse...
Showing: 121 - 140 of 317 results