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We narrowed to 892 results for: CAN

Showing: 701 - 710 of 892 results

  1. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...more concurrent projects in varying stages, which can get messy if you’re not on top of it. In a science-based...yourself with knowledge of software development so you can pick up the jargon and know the concepts as soon ... technology tools with the customer in mind. You can follow her on twitter @rgrubinstein. Additional resources...teams that run within the agile framework If you can’t find internships at science companies, try to find...themes and customer pain points we’ve identified. I can’t wait to send it out. 4:00 PM--Respond to customer...

  2. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...world by storm. Like RNA interference, these enzymes can knock down RNA without altering the genome, but Cas13s..., the Cas13d orthologs described in these papers can independently process their own CRISPR arrays into... require a protospacer flanking sequence, so you can target virtually any RNA sequence! In bacteria, Cas13d-mediated...efficiency; where shRNA treatment produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9...

  3. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...serum There are a few different ways the antibodies can be isolated from the serum. Protein A/G purification... the immunogen. A different type of purification can be performed to isolate antibodies only recognizing...different epitopes of the target protein. While this can increase background staining (see next section), ...

  4. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...components are used to package lab grown viruses. It can be intimidating at first to look at so many plasmid...). VSVG is a broad tropism envelope protein that can also be pseudotyped to alter infectivity. Next, the...While not required for lentiviral production, they can be helpful for certain applications and aid in ease...

  5. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ... sex of their cells when doing experiments—which can lead to failed research. For example, an international...cells and cell lines degenerate over time—and sex can be hard to determine. To remedy this, Shah et al.... been made in this area in recent years, and you can see a summary of this literature in our health and...of cardiovascular disease, 126,000 fewer breast cancers, and 145,000 more quality-adjusted life years. ...Shah et al., “Do you know the sex of your cell?” I can’t tell you how many researchers I’ve talked to who...the most commonly used cell lines appearing in American Journal of Physiology-Cell Physiology over the...product design. But eyes have been opened—and we cannot return to a world that ignores gender. Many thanks...

  6. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...for a curated list of coregulators). Coregulators can function as coactivators and corepressors depending...complexes, a member of a single coactivator complex can serve as a rate-limiting conduit to control the actions...and reagents in the field. Additional information can be found in the Datasets and Reagents sections of...of therapeutic indices, including hypertension, cancer, diabetes, cardiovascular disease, cholesterol ...

  7. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ... template to Cas9 Homology-directed repair (HDR) can be used to make precise modification in genome editing...every 8 bp). Nme2Cas9 is also small enough that it can be packaged into AAV. The lab used single-AAV delivery...base editors Nme2Cas9 CRISPR interference in Candida albicans E. coli genome-wide inhibition library To...All-in-One AAV constructs CRISPR interference in Candida albicans A new CRISPR interference (CRIPSRi) platform...platform for targeted gene repression in Candida albicans was developed by Rebecca Shapiro’s lab. After testing...libraries also include 2,400 gRNAs without any significant hit across the E. coli genome as a negative control... fold and >3,800 fold in human cells with no significant reduction in editing efficiency. Read the paper...

  8. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...Anyone affiliated with the recipient organization can create an Addgene account and place an order: a purchasing... How do I pay by purchase order (PO) number? You can find answers to other ordering questions here. In...it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents that are sent...

  9. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ... features we can offer online for Addgenies and to serve external customers. I also can’t wait to see ...DNA distribution to ensure scientists everywhere can access plasmids despite the evolving export/import...one of the main reasons I have so few worries and can often focus on the excitement of what we are accomplishing... employee pool and we get a lot of successful candidates from internal referrals. Our long reaching reputation...

  10. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...I ended up at pgEd, what I do there, and how you can set yourself on a path towards a similar position...turn crucial if we want to make sure that everyone can be informed and empowered to make their own decisions...ability to talk about science in a language that can be understood by those who are not experts in particular...
Showing: 701 - 710 of 892 results