We narrowed to 904 results for: CAN

...world by storm. Like RNA interference, these enzymes can knock down RNA without altering the genome, but Cas13s..., the Cas13d orthologs described in these papers can independently process their own CRISPR arrays into... require a protospacer flanking sequence, so you can target virtually any RNA sequence! In bacteria, Cas13d-mediated...efficiency; where shRNA treatment produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9...

...more concurrent projects in varying stages, which can get messy if you’re not on top of it. In a science-based...yourself with knowledge of software development so you can pick up the jargon and know the concepts as soon ... technology tools with the customer in mind. You can follow her on twitter @rgrubinstein. Additional resources...teams that run within the agile framework If you can’t find internships at science companies, try to find...themes and customer pain points we’ve identified. I can’t wait to send it out. 4:00 PM--Respond to customer...

...components are used to package lab grown viruses. It can be intimidating at first to look at so many plasmid...). VSVG is a broad tropism envelope protein that can also be pseudotyped to alter infectivity. Next, the...While not required for lentiviral production, they can be helpful for certain applications and aid in ease...

...serum There are a few different ways the antibodies can be isolated from the serum. Protein A/G purification... the immunogen. A different type of purification can be performed to isolate antibodies only recognizing...different epitopes of the target protein. While this can increase background staining (see next section), ...

... sex of their cells when doing experiments—which can lead to failed research. For example, an international...cells and cell lines degenerate over time—and sex can be hard to determine. To remedy this, Shah et al.... been made in this area in recent years, and you can see a summary of this literature in our health and...of cardiovascular disease, 126,000 fewer breast cancers, and 145,000 more quality-adjusted life years. ...Shah et al., “Do you know the sex of your cell?” I can’t tell you how many researchers I’ve talked to who...the most commonly used cell lines appearing in American Journal of Physiology-Cell Physiology over the...product design. But eyes have been opened—and we cannot return to a world that ignores gender. Many thanks...

...for a curated list of coregulators). Coregulators can function as coactivators and corepressors depending...complexes, a member of a single coactivator complex can serve as a rate-limiting conduit to control the actions...and reagents in the field. Additional information can be found in the Datasets and Reagents sections of...of therapeutic indices, including hypertension, cancer, diabetes, cardiovascular disease, cholesterol ...

...Anyone affiliated with the recipient organization can create an Addgene account and place an order: a purchasing... How do I pay by purchase order (PO) number? You can find answers to other ordering questions here. In...it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents that are sent...

... template to Cas9 Homology-directed repair (HDR) can be used to make precise modification in genome editing...every 8 bp). Nme2Cas9 is also small enough that it can be packaged into AAV. The lab used single-AAV delivery...base editors Nme2Cas9 CRISPR interference in Candida albicans E. coli genome-wide inhibition library To...All-in-One AAV constructs CRISPR interference in Candida albicans A new CRISPR interference (CRIPSRi) platform...platform for targeted gene repression in Candida albicans was developed by Rebecca Shapiro’s lab. After testing...libraries also include 2,400 gRNAs without any significant hit across the E. coli genome as a negative control... fold and >3,800 fold in human cells with no significant reduction in editing efficiency. Read the paper...

...Sometimes the PCR products can look smeared, so having an undigested control can help distinguish between.... Cas9 delivery Overview: Before any editing can begin we need our A549 cells to express Cas9. In ...Addgene viruses are provided at a specific titer, you can better plan your transductions and use a range of...well dish. My protocol with many additional tips can be found here. Figure 3: A surprise for the author... implicated in several human cancers) in A549 cells (a human lung cancer cell line), armed only with viruses... CRISPR, I was able to knockout BRAF in a human cancer cell line in about 1 month, which I’d say is pretty...

...I ended up at pgEd, what I do there, and how you can set yourself on a path towards a similar position...turn crucial if we want to make sure that everyone can be informed and empowered to make their own decisions...ability to talk about science in a language that can be understood by those who are not experts in particular...