We narrowed to 932 results for: Gene

...Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page Find... heat loving) bacteria, such as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t work well at...try, as always, the plasmids are available from Addgene. Happy CRISPRing! References Harrington, Lucas...bioRxiv (2017): 138867. Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome... Find CRISPR plasmids at Addgene ...

...sometimes unclear or missed altogether. Emailing Addgene might not be as high on the published-paper-celebration...in this status — a win-win situation for all. Addgene will release plasmids on hold if they are clearly...celebrate!), send us a quick email at deposit@addgene.org with the citation and your deposit or plasmid...distribute unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share... for reading, and for depositing plasmids with Addgene! We'll continue to review our policies to make ...website, Help Center, or email us at deposit@addgene.org. ...

...involves amine-reactive biotin derivatives that generate covalent bonds with positively-charged amino acids...solution was to exploit the best of both worlds. We generated a plasmid that produces biotin ligase and a protein...the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful. Nothing more ...pMal-T-Avi-His/BirA plasmid now available through Addgene. We have successfully used this plasmid to biotinylate...cell signaling.     Additional Resources on the Addgene Blog Check out other Hot Plasmids Find Additional...Posts on Plasmid Technologies Resources at Addgene.org Learn about Popular Plasmids in the Repository...

...about pseudotyping Resources on Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV...coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants. Yet...Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research. “I hope...j.cell.2021.03.013 Additional resources on the Addgene blog Browse all COVID-19 related blog posts Learn...-COV-2 pseudotype virus plasmids from Addgene ...

...language is ... The general representation of data is... The figures are ... In general, I think that this...language in the first (and clunky) version of my general comments and adjust them if my opinion changed ...can be deposited in the specific repositories (Addgene, WormBase, Arabidopsis.info). These repositories...PMCID: PMC4528400. Additional resources on the Addgene blog Find blog posts on scientific sharing Get...tips on writing a review article Resources on Addgene.org Learn about depositing plasmids pre-publication...

...writing for a general audience. The tool highlights jargon terms or words rarely used in general writing in... defining terms. The phrases in Phrasebank are generic, so they need to be edited to suit your particular...desired formatting style. This tool is great for generating citations for a few papers at a time. Mendeley...method in grad school Additional resources on the Addgene blog: Tips for writing scientific manuscripts:...writing a scientific review article   Write for the Addgene Blog ...

...Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around...the next twenty years. But learn we did! From Addgene's very beginning, we had to consider digital accessibility... accessibility on our website.  As members of Addgene’s content team, we — Jo, Brook, and Angela — most... in a poster compared to an online abstract or Addgene blog post.  Here, we are presenting this image ... with the rest of the workshop.  For Figure 1, Addgene chose this alt text: "The left panel is a profile...writing!   Resources Additional resources on the Addgene blog Five Steps to Writing Grant Proposals to Grad...

... Cytometry Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody Collection     ...single color controls On a flow cytometer, you generally want your positive cells to read between 104 and...isotype controls, while others don’t. However, the general consensus seems to be that if you are looking for...Harvard Medical School.   More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies...

...communication and epigenetics. Follow her on twitter @kc_palozola.     Additional Resources on the Addgene Blog ...keep track of the files that I was continuously generating.  I quickly found that there is no one way to...physical lab notebook, along with any figures generated from the analyses: Goal: Before you begin, briefly...control your commonly used scripts: If you edit a general purpose script for a single use, save the script...Software Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find...

...interaction.  In as little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment...point, but one minute of labeling with BASU still generated a >30-fold enrichment compared to E. coli’s BirA...host protein critical to Zika replication. Human genetic disease. RaPID can also be used to test how RNA... answer? The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, ...PMCID: PMC4054858.   Additional Resources on the Addgene Blog Study Protein-DNA Interactions with CUT&RUN...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids...