We narrowed to 927 results for: Gene
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TypeBlog PostPublishedApril 28, 2021, 1:15 p.m....writing for a general audience. The tool highlights jargon terms or words rarely used in general writing in... defining terms. The phrases in Phrasebank are generic, so they need to be edited to suit your particular...desired formatting style. This tool is great for generating citations for a few papers at a time. Mendeley...method in grad school Additional resources on the Addgene blog: Tips for writing scientific manuscripts:...writing a scientific review article Write for the Addgene Blog ...
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Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m.... Cytometry Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody Collection ...single color controls On a flow cytometer, you generally want your positive cells to read between 104 and...isotype controls, while others don’t. However, the general consensus seems to be that if you are looking for...Harvard Medical School. More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies... -
Clear and Accessible Writing
TypeBlog PostPublishedFeb. 15, 2024, 2:15 p.m....Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around...the next twenty years. But learn we did! From Addgene's very beginning, we had to consider digital accessibility... accessibility on our website. As members of Addgene’s content team, we — Jo, Brook, and Angela — most... in a poster compared to an online abstract or Addgene blog post. Here, we are presenting this image ... with the rest of the workshop. For Figure 1, Addgene chose this alt text: "The left panel is a profile...writing! Resources Additional resources on the Addgene blog Five Steps to Writing Grant Proposals to Grad... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m....communication and epigenetics. Follow her on twitter @kc_palozola. Additional Resources on the Addgene Blog ...keep track of the files that I was continuously generating. I quickly found that there is no one way to...physical lab notebook, along with any figures generated from the analyses: Goal: Before you begin, briefly...control your commonly used scripts: If you edit a general purpose script for a single use, save the script...Software Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....Mess in IHC Resources on Addgene.org Browse ready-to-use antibodies at Addgene...visualize a Y-shaped protein reminiscent of the Addgene mascot Abi? If so, you are not alone. Full-sized... divide up the different regions, both through genetic and proteolytic approaches. This comes in handy...when that might be later). Find scFv plasmids at Addgene Depending on how an antibody is broken up, there...j.crmeth.2022.100301 Additional resources on the Addgene blog: Introduction to AntibodiesSingle Chain Fragment... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m.... Resources on Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene... functionality of the conjugate. For example, Addgene’s antibodies are provided in a buffer with the antimicrobial... Schibli R (2015) Antibody Conjugates: From Heterogeneous Populations to Defined Reagents. Antibodies ...10.1038/nmeth.4380 Additional resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....interaction. In as little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment...point, but one minute of labeling with BASU still generated a >30-fold enrichment compared to E. coli’s BirA...host protein critical to Zika replication. Human genetic disease. RaPID can also be used to test how RNA... answer? The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, ...PMCID: PMC4054858. Additional Resources on the Addgene Blog Study Protein-DNA Interactions with CUT&RUN...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m.... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...antibodies work? Secondary antibodies are antibodies generated against antibody isotypes from a specific species...classes, it is possible to get secondary antibodies generated against isotype subclasses, such as mouse IgG2...my goat anti-rabbit?!”* *a secondary antibody generated in goat against rabbit immunoglobulins. References...References and Resources More resources on Addgene’s blog Antibodies 101: Introduction to Immunofluorescence... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to address irreproducibility... to Teytelman’s connection with Joanne Kamens, Addgene also had the opportunity to contribute to this ...being adopted by a wider group of researchers. Generating material to empower others to practice and teach...anyone could present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing...Tsang for editing. Additional resources on the Addgene blog: Check out this guide on Transferable Skills...workshops Levelling Up: Reproducibility for Everyone Addgene: a global resource working to enhance scientific... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....placed on the Addgene website, it sets off a chain reaction of events throughout all of Addgene - within the... of thousands of plasmids a day, like we do at Addgene, pen and paper will fail you. The benefits of lab...fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software tools to ...krisshamloo.com Additional Resources on the Addgene Blog Learn about How We Use Barcodes in the Lab...Track Your Experiments Additional Resources on Addgene.org Find Plasmids for Your Research ...