We narrowed to 904 results for: CAN

...and enable scientists to discover if known drugs can be used to treat these diseases (drug repositioning... found that low concentrations (≤3 mM) of GdnHCl can functionally rescue multiple Arg-mutant proteins ... developmental delay. Mutations in NFIA and NFIB can also cause developmental delay in patients. We have...physiological and pathological roles of human cell glycans. Accordingly, she joined efforts with the Portuguese...They result from defects in different steps in the glycan biosynthetic pathway and have a wide range of clinical...which leads to nearly 25% child mortality. Since glycans play an essential role in almost every aspect of...NFIX gene and test whether variants of unknown significance (VUSs) that are frequently found in genetic ...

... complicated solution like cell lysate. But they can be a lot of work — and what if you want to detect... stripping is here! Rather than start over, you can remove the detection antibodies from your membrane...brittle, so proceed with caution. Unlike PVDF, they can be returned directly to buffer without a re-wetting...

...susceptible to redox modification, the MPST hydrosulfide can be transferred to the roGFP2 cysteine thiols and ...change its fluorescence properties. The biosensor can also be used along other thiol-containing acceptors...further enrichment. Tian notes that this platform can be used to engineer many types of biosensors.   This...the brain. “We hope that iGluSnFR3 will enable significant steps forward in our understanding of synaptic... Brandán Pedre Pérez is a postdoc at the German Cancer Research Center (DKFZ) who has developed new redox...withdrawal Elizabeth Hubbard is a fourth year Ph.D. candidate at the University of California, Irvine where ...

... features we can offer online for Addgenies and to serve external customers. I also can’t wait to see ...DNA distribution to ensure scientists everywhere can access plasmids despite the evolving export/import...one of the main reasons I have so few worries and can often focus on the excitement of what we are accomplishing... employee pool and we get a lot of successful candidates from internal referrals. Our long reaching reputation...

... to thymine Since an enzyme that excises inosine can be modified to target guanosine instead, perhaps ...editors, called gTBE and gCBE, with Addgene. gTBE can introduce T → C and T → G edits, though with limited... CGBEs. This distinct approach means researchers can now select the best tool for their application from...options.  Conclusion With these advances, every base can now be directly targeted by a base editor, although... accurate, more efficient, and more diverse. We can’t wait to see what tools the field will come up with...

...training in a globalized world where individuals can exchange ideas, network, collaborate, and innovate...of people hold a PhD, achieving our career goals can be a real challenge. On top of that, in the midst... (s)he feels truly understood and supported, and can more fully identify with and be inspired by the mentor...a mentor who has experienced them before and who can serve as a role model and advisor. For their own ...research at UK in the same field that I do: prostate cancer. She has guided me in my next steps. For example...that their knowledge and skills had improved significantly when compared to their pre-program self-assesment...improvements, not necessarily quantifiable, but always significant. Doubts, fears, insecurities… all shrink when...

...microscope is not the only way to visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro...amoebasisters.tumblr.com/post/139944465237/lactose-around-we-can-make-some-enzymes-for   The take home message was...listening to talks all day. But nope, not at the American Society for Microbiology 2018 Microbe meeting....

...protein B; but proteins are social biomolecules. They can be part of multiple dynamic and overlapping complexes...is pulled down, typically with an antibody. This can artificially select against PPIs that aren't stable...protein fragmentation complementation assay that can be used to study binary protein interactions. However...generally only tells you if two proteins interact and cannot be used to identify interaction partners other ...protein-protein interactions, SplitBioID provides a candidate list of proteins that are part of the bait proteins...interactome. Further characterization of these candidates is critical to understanding how they interact...

...all AAVs that reproducibly labeled retinal cells can be found here.  Applications for synthetic promoter...specific cell types, pairs of synthetic promoter can be used to restrict expression to a single cell type...Cre-mCherry and GFP.   Figure 2: Synthetic promoters can be paired for targeted expression. This same pairing...

...optimization of the exon-splitting site sequence can enhance the rate of trans-splicing (Lai et al., 2005...sequences and the homologous region of the two vectors can be from one of the transgene’s endogenous introns...2014). Drawbacks: The choice of homology sequence can influence the level of transgene expression, and ... capable of expressing a truncated protein. This can be avoided by excluding a stop codon from donor A..., was 81% of that from a single vector, but significantly greater than the expression from an overlapping... the first HDR event, then the second HDR event can’t occur. Lastly, depending on its design, donor A ...