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We narrowed to 892 results for: CAN

Showing: 731 - 740 of 892 results

  1. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ... conventional PCR. Biallelically disrupted cells can be easily identified. Even in the case of clones ...of many available strategies by which biologists can imagine, create, and examine novel genetic configurations...post was contributed by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical...deletions in mammalian cell lines. Below Bauer and Canver discuss the motivations behind this research.   ... more at his webpage, bauerlab.org.     Matthew Canver is an MD/PhD student at Harvard Medical School ...Science 2013 Oct 11;342(6155):253-257. PubMed. 3. Canver MC, Bauer DE, Dass A, Yien YY, Chung J, Masuda ...

  2. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...weak, government support is “feckless”.  So what can be done?  He charged the postdocs to “Grab ahold ...destinies” and also suggested some concrete actions that can be taken now to solve some of the more “tractable...Workforce, Metrics and Incentives and Funding.  I can’t wait to see what solutions these talented postdocs...science to be happy so the beginnings of solutions can’t come too soon.  Watch the Future of Research Website...

  3. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...no doubt that companies are swarming to see what can be done with this technology to help patients. Dr... me, I continue to be amazed at how cool biology can be and how much we have yet to learn. As Dr. Mello... cure sickle cell anemia in stem cells but this can’t be used in people until we learn more about safety...

  4. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...infection first of Cas9, then with the sgRNAs, and can result in a higher titer due to the smaller size ...application offer proof-of-principle that CRISPR-Cas9 can be used to target any sequence in the genome. Tips... identify genes essential for cell viability in cancer and pluripotent stem cells. In a melanoma model...in search of genes involved in resistance to the cancer drug vemurafenib. Enriched libraries for select...

  5. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...with a flexible linker where a protein of interest can be inserted. B) Protein expression and labeling procedure...voltage indicator (GEVI) than previous versions. ASAP5 can be imaged using one- or two-photon microscopy to ...Altogether, if you want a negative-going GEVI that can be used in different illumination regimes with fast...with non-neurodegenerative diseases (Diabetes & cancer). Image adapted from Wu and Wang 2024 under a CC-BY...

  6. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...ve ever had an MRI, you know that magnetic fields can pass right through our tissues unimpeded. Andrew ...engineering approaches and that magnetosensitive tools can be developed from other fluorescent proteins.  Currently...optogenetic tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic...Maria’s screens of AsLOV2 variants showed some significant gains! After a few more rounds of mutagenesis...ideas and are happy to speculate, they know they can’t answer these questions alone. They readily admit...Confined Persistent Diradical Emitter. Journal of the American Chemical Society, 145(25), 13615–13622. https:...

  7. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...confidence and learn to work independently, which can lead to greater success in meeting educational goals...without challenges. Real research is messy, and it can be difficult to give up the control embedded in “... Course-based undergraduate research experiences can make scientific research more inclusive. CBE Life...the training of the mind to think something that cannot be learned from textbooks” (Brian, 1996). But is...citrate synthase and presented their findings at the American Society for Biochemistry and Molecular Biology...

  8. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...science outreach, not only can I easily share science with the public, but I can watch and learn from the...dinosaurs every day. They made me happy. Grad school can provide plenty of negativity, so I volunteered at...wonderfully supportive mentor and graduate office. I cannot stress how important they were to my success as...

  9. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... data easily available, organized in a form that can be understood by others, balanced against how often...rarely preserve “raw” data of any sort in a form that can be parsed by someone other than the actual author...Addgene helps with DNA reagents, these resources can offload the burden of data storage and distribution... storing data in a sharable form is indeed a significant effort. Our standards of data recording are highly...Research Institute at Mount Sinai Hospital, Toronto, Canada where he studies protein-serine/threonine kinases...

  10. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...You can use viral vectors for many experimental purposes. To help you make sense of all the viral vector...
Showing: 731 - 740 of 892 results