...Resources on the Addgene Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate... Generate Insertion and Deletions in a Plasmids Learn How to Verify Your Plasmid Resources on Addgene.org...

...in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to...Fluorescent proteins (FPs) are the gold standard for genetically-encoded FRET donors (Fig. 1). Scores of FP variants...presented (Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already...Gradia Lab has deposited a gateway vector with Addgene, where you can insert your protein of interest,...PubMed Central PMCID: PMC4380784. Resources on the Addgene Blog Get Tips for Using FRET in Your Experiments...Your Fluorescent Protein Basics Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids...

...Fatima's work and keep checking back in with the Addgene blog for future award opportunities. Xin Zhou:...when exposed to ultraviolet light. To do so, she generated fusion proteins in which Dronpa domains flanked...Proteins, or FLiPs.  Xin later optimized these first generation FLiPs by mutating Dronpa to lower its aggregation...Chemical Biology! Additional Resources on the Addgene Blog Learn More about Photosactivatable Fluorescent...Fluorescent Protein for Your Experiments Resources on Addgene.org Find Fluorescent Protein Biosensors Browse Plasmids...

...specifically how dry ice can affect virus from Addgene. Bottom line: there’s good news and there’s bad... results. Order Ready to Use Viral Preps from Addgene Measuring the pH of our samples: What we did To... recommend that once you receive your AAV from Addgene, you store the AAV at -80°C for 96 hours prior ... PMCID: PMC4420252 Additional Resources on the Addgene Blog Learn How You Can Use the Viral Service Tips...Your Lentivirus Preps Additional Resources on Addgene.org Check out the Viral Service Find Virus Protocols...

...external collaborations Resources on Addgene.org Browse Addgene's COVID-19 plasmids and resources ...efforts to shift scientific culture towards a more generous and rewarding way of making science.   How...currently based in Spain and works in the field of genetics and cancer. You can follow her on Twitter at @...mahesa2504         Resources Additional resources on Addgene's blog Check out our blog post about remote collaborations...

...reading, you can calculate the DNA concentration. Generally, A260 of 1.0 is equivalent to 50 ug/ml pure dsDNA...samples and are often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, .... This method is often used preceding next-generation sequencing or microarray studies and is not used...suited than another. Additional resources on the Addgene blog Read more about molecular biology protocols...different grades of plasmid DNA preps Resources on Addgene.org Visit the Molecular Biology Reference Find plasmids...

... Labeling in Flow Cytometry Resource on addgene.org Addgene's Antibody Guide rAb Affinity Purification...Prathima has a research background in protein mutagenesis and cancer immunotherapies. She is currently ...Harvard Medical School.   More resources on the Addgene blog Antibodies 101: Flow Cytometry Conventional...Purification Protocol Video rAbs in the Addgene repository...

...your foot in the door Resources on Addgene.org Check out how Addgene uses videos for science communication...yourself how every piece of feedback you receive generalizes.  If someone suggests a change to a slide, think... science communication, including the teams at Addgene, ComSciCon, and many more great organizations. ...entertainment industry.       Additional resources on the Addgene Blog Learn about careers in science communication...

... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics:...antibody for a…less sensitive moment.   Fig 1: Blugene fading into darkness because they accidentally ...accidentally left their antibodies out. It's okay, Blugene!    Buffers  You know what temperature to store your.../help/spectral-overlap/   More resources on Addgene’s blog Antibodies 101: Monoclonal Antibodies Antibodies...

.... Different setting, different population, new genetic variation, or different handling can all be important... tested with a new experiment; and hypothesis generation, in which data are investigated to look for unexpected...trends or differences between groups. Hypothesis generation is critical for advancement, but we are too often...using hypothesis testing statistical tools that generate a p-value.  As an example, if you hypothesize ...clear distinction between hypothesis testing and generation, can be sure that results will be reported regardless...