We narrowed to 791 results for: Cre
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TypeBlog PostPublishedFeb. 4, 2014, 3:50 p.m....can make a big difference, if you can find one. Create your own peer mentoring circle. This type of program...
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Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....Roth BL (2007) Evolving the lock to fit the key to create a family of G protein-coupled receptors potently... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report electrical... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....highlights of 2018 for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing TRIM21, finding... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....neurodegeneration. The level of this protein is decreased in Alzheimer’s disease patient’s brains. However... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....proteins are available, and their number is steadily increasing. Of course, you want the ‘latest and greatest... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....more efficient, as one does not have to thoroughly screen colonies for the insert. Gateway® technology (developed... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....protein-protein interactions with other peptides. Created with BioRender.com. Many biologists can... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....limitations. New efforts have recently been made to increase cutting efficiency and diversify PAM sequences...