We narrowed to 939 results for: Gene

...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  ...Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ...that interact with nucleic acids. Labs studying epigenetics routinely use the chromatin IP (ChIP) technique... found this blog helpful be sure to check out Addgene’s blog for other antibody topics.     References...

...partnered with Addgene to make our collection of integrin antibodies available through Addgene’s repository...10.1038/s41467-022-35173-w More resources from Addgene Addgene's Antibody Guide Antibodies 101: Flow Cytometry...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...on these cells. What’s next IPI is working with Addgene to accelerate biomedical research by distributing...

...Science Communication Resources at Addgene.org Check out Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small tasks (like... preparing plasmids to send to Addgene). Following my advice from above, you should probably...appropriately. Back in my undergrad days, I had general goals for my experiments, but didn’t spend enough... in the comments! Additional Resources on the Addgene Blog Management for Scientists eBook Get Advice...

...targets.  The CRISPRecise kit available at Addgene includes 17 IFNs, covering a spectrum of activity... Rough screening of nuclease variants by next-generation sequencing (NGS) After cloning your sgRNA: Check...shown.  This second round of screening will generally provide off-target-free editing. (Note: this conclusion... approach, highly specific genome editing can generally be achieved without using genome-wide off-target...s41467-019-12281-8  Additional resources on the Addgene blog CRISPR 101: Off-Target Effects Enhancing ...Genome-Wide CRISPR Screen Design Resources on addgene.org The CRISPRecise Kit page includes detailed ...

...engineered ascorbate peroxidase (APEX), used as a genetic tag for electron microscopy, could be used for ...(Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development of BioID and APEX, optimized...These newer tools are also currently available at Addgene.  BioID2: A smaller improved promiscuous biotin...utilized yeast display-based directed evolution to generate two mutants, the 35 kD TurboID, which has 15 mutations...et al. "Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy." Nature...PMCID: PMC5467174. Additional resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101...

...remotely instead of from the lab. Nonetheless, Addgene’s annual Deck the Lab competition drew upon the ...Decoration Competition! #ScienceFun #ZoomParty @Addgene pic.twitter.com/drNBUdlQco — The Tarling-Vallim...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com/GsAlD6sjM6 — Dr. Anne A Madden ...thank you to everyone who entered. Keep an eye on Addgene’s social media throughout the year for more fun ...

...ways of generating antibodies, for example through the use of less toxic adjuvants; 2) generating complex...and 4) generating mAbs against new cancer biomarkers. We believe that repositories, such as Addgene and ...The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years Data Freedom: The Expansion of...

...Selecting the Right Antibody Resources on Addgene.org Addgene's Antibody Guide  ...controls for biological experiments, starting with general controls and then moving on to controls for antibody-related...interpretation of results. A positive control is generally a sample or group that will have a desired response...address this, block positively charged groups with generally sticky proteins such as bovine serum albumin. ...Central PMCID: PMC2712964. More resources on the Addgene blog Antibodies 101: Validation Antibodies 101:...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...please let us know! Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...into lentiviral vectors to generate a kit which is now available at Addgene. “We called it cloning by ...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...PMCID: PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled...