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TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m....research, they are now seen as an ubiquitous tool that can be diversely applied to many different experiments...Ed.) eBook And now for some exciting news... You can now download the newest version of Addgene's Plasmids...
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Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....to engage in extracurricular activities (EA). EAs can: Introduce new perspectives in science and engineering...the big picture of the project. For example, how can future regulations impact your work? What design ... -
Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!
TypeBlog PostPublishedApril 8, 2021, 7:25 p.m.... stats and more on the addgene.org homepage! You can track the number of plasmids right below the search... -
Antibodies 101 Series on TikTok
TypeBlog PostPublishedMarch 19, 2024, 1:15 p.m....shared on our @addgene Instagram account, or you can watch the full animation on our YouTube channel. ... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....cases, the selected mutation must be removed, which can be done by isolating animals with particular visible...become even more powerful following a report that one can use linear repair templates (ie. PCR-derived dsDNA...precise knock-ins, and homozygous knock-in animals can be obtained in eight days. The only animals on a ...2017). Because C. elegans has an AT-rich genome, it can be difficult to find an appropriate NGG PAM site ...repair of a temperature-sensitive pha-1 mutation significantly enriches for knock-in of 2x and 3xFLAG epitopes... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....itself a versatile option for tagging proteins, as it can be fused to the N- or C-terminus (or even in the ...., 2024 under CC BY license. This construct can be easily expressed and purified from E. coli, retains...light source, they have lower background signal and can be used in light-sensitive systems. Most excitingly...2024 American Chemical Society. Both PILATeS (18 kDa) and STReTCh (11 kDa) are significantly smaller... revealing antigenic differences between human, canine and simian isolates. J Gen Virol., 68 (Pt 11), ...expressing it with additional cofactors or using significantly truncated forms that limit its functionality... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog PostPublishedNov. 19, 2020, 2:15 p.m....tools so that both veteran and new Rett researchers can find the reagents and resources they need to cure...for the first time that programmable RNA editing can repair a gene in a living animal across multiple ...are available in the Resource Center. Researchers can also find links to cell line repositories including...models, Rett syndrome is a strong translational candidate for gene replacement therapy. In 2014, RSRT brought... the work of the consortium, two gene therapy candidates advanced into clinical development programs in... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....general guide to the effect on higher education, you can see that those in teaching roles (such as adjuncts...communications with and research on these institutions, we can say with relative certainty that just over 40% of...at some institutions; and that institutions which can more easily provide bridge funding to absorb the ...postdoctoral workforce in the sciences. The Dana Farber Cancer Institute (at Harvard) and MIT BCS (Department ... and sustainable research ecosystem and that we cannot ignore these changes if we wish to minimize the... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....the brain. With the advent of optogenetics, ICSS can now be done with neuronal cell-type and temporal ...remote memory retrieval) specificity. Optogenetics can be used to more precisely associate regions of the...behavioral output with optogenetic manipulation, this can be explained primarily in two different ways 1) your... -
Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab
TypeBlog PostPublishedSept. 7, 2021, 1:15 p.m....here for you as you learn the ropes! All material can be found on our Protocols page and YouTube page. ...