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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 892 results for: CAN

Showing: 761 - 770 of 892 results

  1. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...address these issues using our existing resources. You can read it, and other case studies, on the Seqera website...

  2. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...the most skeptical reader of historical fiction (I can personally attest to this!). Following history from..., we’ve read books across nearly every genre you can imagine: gothic, mystery, sci-fi, fantasy, pop-sci...

  3. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...oligomerization of biotinylated proteins on avidin can be controlled easier than formation of GST dimers...fusion protein from a bacterial extract. The MBP tag can be removed using Xa protease. The carboxyl-terminus...extent, the number of added biotins per molecule cannot be well-controlled. The lack of site selectivity...We are clearly NEB “fanboys” as the bacteria significantly enhance protein folding (tested using real science...

  4. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...enhance Collections so that a group or organization can annotate their sequences from a shared database of...companion product. If you don’t use SnapGene yet, you can download a free trial. If you notice that a feature...

  5. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ... single field of view for constant tracking. You can then directly monitor multiple rounds of translation...contains 24 copies of SunTag, a synthetic scaffold that can recruit GFP fused to the SunTag-specific antibody...

  6. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...process doesn’t cause double-stranded breaks and can deliver large sequences and even entire genes. ...testing, and approval. In contrast, a system that can deliver a healthy gene as a single intervention for...integration occurs in a single enzymatic step. We can’t wait to see where evoCAST takes us on the high seas...

  7. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...to the plates to select for candidate knock-ins. These candidate animals can then be further screened based...made CRISPR in C. elegans even easier - now, one can generate a fluorescent protein fusion, transcriptional...

  8. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ... growing conditions. As a facultative aerobe, it can thrive with or without oxygen. Additionally, its ...much more. Because of it’s BSL-1 status, E. coli can be used as a model to relate these processes in more...microinjections. Injections of mRNAs, for example, can be analyzed to understand human disease alleles during... norvegicus genes! Mexican axolotl (Ambystoma mexicanum) Ambystoma mexicanum, aka the axolotl. Image... Biosafety Level 1 microbe makes it a perfect candidate for introductory teaching and research environments....  Rattus norvegicus. Image from Staffan Vilcans. Rat physiology is also easier to study and ... Image from AJC1. The Mexican axolotl is one of the most unique model organisms, recognized for its...

  9. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ..., they also found that the signal to noise ratio can be increased from 2-fold (by CRISPRainbow) to 3-fold... hybridization, CRISPR-based visualization tools can be used in live cells. Compared to its predecessor...in CRISPRainbow, they knew they could be good candidates for further development. They inserted an octet...

  10. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...tinker with DNA. Making changes to a DNA sequence can help scientists identify and/or facilitate the evolution... bases, which limits the level of variation that can be introduced.   Diversification is limited due...
Showing: 761 - 770 of 892 results