We narrowed to 927 results for: Gene
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TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m.... Resources on addgene.org Addgene Antibody Guide Ready-to-use antibodies from Addgene More Resources ...Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to ..., Bannwarth W, Döbeli H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant...
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Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....attendees to read and comment online! Want the general public (say, policymakers or community organizations...the manuscript prior to submission. Preprints at Addgene Preprints are all about sharing scientific knowledge...more openly — which is also our mission here at Addgene! Along with other open-science organizations, we...significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in the final journal...10.1371/journal.pbio.3000116 More resources on the Addgene blog Summer SciComm Series: Modes of Communication... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m....training in a variety of transferable skills. At Addgene, we've set out to fill this gap by both highlighting...currently work on the Scientific Support Team here at Addgene, so troubleshooting molecular biology experiments...because it helps me see problem solving as a generalizable process. Step One: Identify the problem ...choose. Resources Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m....Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...started: Subscribe to Career Blog Posts from Addgene 1) Twitter – Get your voice out there Here’s one...today, Soapbox Science (hosted by Nature), and Addgene are open to guest contributions. If you decide ...BananarootsBlog. Additional Resources on the Addgene Blog Check out Other Science Communication Posts... -
How to Prepare for an Industry Interview
TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m....Resources on Addgene.org: Sign up to receive Addgene’s Science Career Guide Check out Addgene’s job openings...interviewer,” you can find lists that have lots of general questions about job responsibilities, performance...but then relax. Additional resources on the Addgene blog: Tips for writing a good cover letter 6 Steps... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....especially when trying to explore the links between genetics and anatomy, which are not yet fully understood...many different mutants because the Caenorhabditis Genetics Center (CGC) at the University of Minnesota has...Makerspaces like ours can also make accounts with Addgene. We now have the incredible CRISPR/cas9 tools at...folks at the makerspace comfortable with next-generation sequencing data and the bioinformatics pipeline...resources to pursue it. Since makerspaces are generally funded on a membership model - everyone contributes... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....and Conjugates Resources on Addgene.org Addgene's Antibody GuideAddgene's collection of ready-to-use recombinant...Overall, spectral flow cytometry has the ability to generate incredible amounts of data from a single experiment...early life. Resources More resources on the Addgene blog Antibodies 101:Selecting the Right AntibodyAntibodies... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....be able to tell you the laser configuration. In general, if two fluorescent markers are read by the same...to become a perpendicular vertical line. In general, most spillover between samples can be fixed with...anyone just starting to use flow cytometry, it’s generally recommended to start compensating with single-... compensation panels below each plot. The general rules of compensation apply to your experimental...Harvard Medical School. More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies... -
Transferable Skills: Negotiation
TypeBlog PostPublishedMarch 3, 2022, 2 p.m....partner with Addgene. What are the most important issues for our partners and how can Addgene work with ...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting...Richard D. Lewis Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m.... overexpress a recombinant protein? It is not generally in a cell’s best interest to overexpress a protein...for affinity and purification An affinity tag, generally a relatively small sequence of amino acids, is...interest would eventually get ridiculous (you generally don’t want more tag than protein), 2-3 tags is...using plasmids found in this paper (available at Addgene). Table 2: Protease recognition sites commonly...purification is provided by GE Healthcare Life Sciences. Addgene pages and blog posts of interest: Which Fluorescent...