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  1. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...fluorescent reporter, such as GFP or mCherry, through genetic modification. As your protein of interest will ...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Introduction to Gating in ...

  2. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...the dye and the endogenous target, and thus no genetically encoded tag is required. These dyes can also ...be SNAP-tag® and HaloTag®. These consist of a genetically encoded enzyme variant tag that covalently reacts...maintained through mechanisms like fission, fusion, biogenesis, and mitophagy, can be tracked using dyes like...Keller, P. J., Ji, N., & Lavis, L. D. (2017). A general method to fine-tune fluorophores for live-cell ....1016/j.bbamem.2013.09.005 More resources from Addgene Fluorescent protein plasmids and resourcesA practical...

  3. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...fluorescent microscopy technique for your study see Addgene’s blog post Which Fluorescence Microscopy Techniques...experiment and are unsure which to choose, check out Addgene’s article: Which Fluorescent Protein Should I Use.... During FACS, the sample stream oscillates to generate droplets that are charged as they pass through.../s10616-011-9415-0 Additional resources on the Addgene blog Check out other methods using fluorescent...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...

  4. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...power of community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific collaboration...sequencing capabilities to complement our next-generation sequencing, setting new standards for plasmid...online and in-person, you helped us understand how Addgene can better serve the global scientific community...about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez...

  5. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Find...Find lab tips for your research Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

  6. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...protein. The pCri system, recently deposited to Addgene by F. Xavier Gomis-Rüth, aims to take the guesswork... either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The ...visualization of the data from RSCB PDB Resources on the Addgene Blog Plasmids 101: E. coli strains for protein...expression Plasmids 101: Protein tags Resources on Addgene.org Find Additional Cloning Kits ...

  7. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased towards... likely due to the distinct overhang patterns generated by the nickases; D10A creates 5’ overhangs and...reviewing this blog post. Additional Resources on the Addgene Blog Learn about Homology Directed Repair 3 Tips...Efficiency How to Design Your gRNA Resources on Addgene.org Check out CRISPR Topic Page Find CRISPR Nickase...

  8. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...first program I used to create graphics for the Addgene blog. However, it has a limited number of drawing...body, cellular biology, medical specialities, and general items. Individual images as well as collections...comments section!  Additional resources on the Addgene blog Read our Early Career Researcher Toolbox ...

  9. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ...scientists here Resources on Addgene.org Learn more about careers at Addgene ...posts in this series! Additional Resources on the Addgene Blog Learn more about alternative science careers...creative science education tools? Check out this Addgene podcast with educator and science rap mastermind...

  10. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...further consolidate these FAQs, Le Cong worked with Addgene Senior Scientist, Matt Ferenc, to organize the ...full list of 16 FAQs answered by Le Cong Read Addgene's CRISPR guide for background information on CRISPR...the most frequently requested CRISPR plasmids at Addgene Find protocols and gRNA design tools: List of...scientific community and sharing these resources with Addgene. Thank you, Le!    ...
Showing: 771 - 780 of 914 results