...methodologies and rigorous research plans. However, amidst the organized chaos of lab research and PhD studies, ... Widening your search The first step towards inviting serendipity into your research lies in expanding...structured world of lab research and PhD studies, where every aspect of research is meticulously planned... the potential to redefine the course of one's research journey — serendipity. The best scientific breakthroughs...enhancing your project's potential. Widening your search creates fertile ground for serendipitous encounters...become very focused on the details of a specific research question. However, breakthroughs often happen ...the constant stream of thoughts related to lab research and gain fresh perspectives. Such activities foster...

...parts of the world in my early research career. I've found that research institutes in different places...I believe that enChIP will markedly accelerate research of regulation mechanisms of genome functions in... enChIP, and I'm very happy to see that many researchers all over the world have requested plasmids for...cells. We believe that this system will allow researchers to use enChIP in a wide variety of biological...promote Addgene, which is also beneficial to my research. I believe that involvement in the scientific ...scientific community is a kind of duty for researchers when considering the peer-review system of journals and ...Addgene: Throughout your career, you have held research appointments in the US (New York University School...

...one visual barcode per cell and up to 200-plex with two barcodes per cell. The researchers validated their...visual barcodes for clonal multiplexing of cells in live imaging application. Each visual barcode consists...visual barcodes in use in A375 cell line. Image from Kaufman, et al, 2022.    This new barcoding technology... error rate for long sequence knock-ins Visual barcodes for live cell clonal multiplexing Improving ...interaction). The modularity of the system provides researchers better flexibility and makes the approach more...generate “single-use” transgenic lines. Instead, researchers can generate and maintain transgenic lines that...error rate. For a one kilobase insertion, the researchers reported an editing error rate of less than 0.3%...

...scientific community speed research is to offer plasmids created as part of research projects, another way ...Consortium collection enables research on solute carriers (SLCs) for medical research Recently, the Michael Davidson...Susanna Bachle and Matt Takvorian When COVID-19 researchers started working with us to help them share their...Transfer Offices at universities to make COVID-19 research-related plasmids available more broadly: to scientists...for-profit setting.  In light of the need for basic research during the current pandemic, universities are ... of support about allowing use of plasmids for research by anyone. As infection rates were increasing ...partners quickly assessed how to more readily enable research. Often “normal” timelines that could be weeks ...

...more into what researchers felt about NgAgo, I conducted a survey and asked researchers if they had tested...Update (November 18, 2016): Researchers from a variety of institutions recently reported their inability...sequence for target recognition, which gives a researcher unprecedented freedom to target any sequence ...form a complex with an ssDNA guide. Therefore, researchers must co-transfect cells with 5’-P-ssDNA guides... in the versatility of its delivery methods. Researchers can conveniently transfect/inject cells with ...requested by labs around the globe nearly 400 times. Researchers have been excitedly testing NgAgo's genome editing...discussions in the forums, it appeared that many researchers were having difficulty achieving indel formation...

...Universities and research institutions can turn to Addgene as a trusted partner for archiving their biological...present an unprecedented challenge to the scientific research ecosystem, not just in the United States but around...organization that directly collaborates with NIH-funded researchers and utilizes grants to support our own initiatives...life-saving medical breakthroughs and undermine critical research infrastructure that has taken decades to establish.These...remains steadfast in our mission to accelerate research and discovery by improving access to high-quality...funding. Addgene is uniquely positioned to help research institutions maximize their increasingly limited...scientific assets: We hold a repository of valuable research materials that might otherwise be lost amid lab...

... like many search engines, featuring both a general search bar tool and an advanced search option. For...   You can also search by protein sequence with BLAST, using the homepage search bar. Once you have...only Cas enzyme widely available and applied by researchers for gene targeting. Fast forward a decade, and...Institute. What is CasPEDIA? CasPEDIA is an online, searchable resource that provides summary information on...section on each enzyme page, you'll find a link to search Addgene's collection for plasmids containing that...  Helpful resources and information For some researchers, the previous information may be all you need...table of protein entry results. For the advanced search options, this is where you can really identify ...

...Balancing research with medical practice as an MD/PhD. Darrel does 20% clinical work 80% research and was... but later got obsessed with research. Chapter 3: How all basic research ends up being translational. ...for Regenerative Medicine accelerates research. Chapter 5: Research currently under way in the Kotton Lab...Chapter 8: The importance of a mix of research approaches. Why researchers should have a mix of high-risk, ...at Boston University Medical School. Darrell’s research focuses on the lungs, and, among other projects...Regenerative Medicine, and within the biomedical research community. Along these lines, Darrell recently...sharing. Listen to learn more about Darrell, his research, and his focus on open science. Listen to the...

...postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. It is commonly cited that...perform in vitro kinase reactions. Often, however, researchers lack knowledge of the regulatory kinase, access... phosphoserine (as described below) provides researchers with a valuable tool for understanding the contribution...curious anomaly amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA... phosphoserine (Sep)-tRNA synthetase enabled researchers to create an orthogonal translation system (OTS...encoding the ORF to be expressed Finally, the researcher needs a plasmid encoding their open reading frame...non-phospho-incorporated proteins purified from the system, researchers can run samples on SDS-PAGE gels containing Phos-Tag...

...has the sequence of the mutated gene the researcher is searching for), then the probe will bind to the sample...of DNA used saving the researcher both time and resources (3). Still, researchers generating transgenic...experiments allowed researchers to identify the location of genes. With this knowledge, researchers were able to...at the end as in standard PCR. This allows the researcher to compare different DNA sequences to see which...In the era before sequencing was commonplace, researchers used restriction mapping to investigate DNA sequence...running the products on an agarose gel allows a researcher to properly orient specific fragments of a DNA...This approach to studying DNA sequences helped researchers at a time when sequencing was still in the early...