We narrowed to 904 results for: CAN

... protein tag which can be added to either the N or C terminal of a protein and can inducibly facilitate...Protein degradation can be induced by the addition of a small molecule, and protein levels can return to normal... concentration can yield intermediate protein abundance. Finally, some degron tags can do more than just...you can’t predict with absolute certainty how a degron will behave with a new protein, but you can make... Here we will review what advantages degron tags can offer over other protein control methods and compare...addition of an exogenous small molecule). Degron tags can range in size from several kDa up to ~35 kDa and ... unlike many other methods. Secondly, these tags can be used to manipulate endogenously and exogenously...

...for recombineering can be found in Thomason et al and Sharan et al (a few strains can also be found at ...Colony PCR can be used to screen for positive clones in most cases, and restriction enzyme digest can be used...biggest limitations is that sequence modifications can only be made at restriction enzyme cut sites. The... lambda red system is an alternative method that can be used for cloning or genome engineering and is ... recombination-mediated genetic engineering.  It can be used to make an assortment of modifications: insertion...a few base pairs.  dsDNA Substrate dsDNA inserts can be made by PCR using primers that amplify the DNA...introduced during PCR. ssDNA Substrate ssDNA substrates can be generated by ordering synthetic oligonucleotides...

...understanding the scientific process can help us understand how new therapies can be developed and applied. The... it can be tempting to try to plan out a very distinct and certain path, embracing uncertainty can help...for undergrads, or anything else you can think of to ask! You can DM me on Twitter @EleanorWang. I will...too. You can follow her on Twitter @EleanorWang.     References and resources References Canning EA, LaCosse...the first step into the world of academic research can be quite daunting. During my first year of college...continue my scientific endeavors. I hope that this post can provide some perspective and advice to any undergraduate...strange and exciting revelation. Science in any field can generally be applied to improve our lives and solve...

...Pray, 2008). Scientific discoveries in yeast can then can be further studied in other model organisms ...transparent. Thus scientists can easily observe early developmental stages which can be difficult in other vertebrate...set of mutations that made cancer cells more resistant to chemotherapy. You can find these plasmids at Addgene...reproduce as often as every three weeks so scientists can simultaneously observe several generations of mice...organisms is that they are genetically tractable. Mice can be easily manipulated with tools like CRISPR to make...straightforward to manipulate genetically. Scientists can make a new line of flies in only 6 weeks compared...expression is possible in other model systems but can be quite difficult and time consuming. GAL4/UAS was...

...3 you can see how I gated out my lymphocyte population from a tumor sample. Many cell types can be found...used a fluorescent dye that can only enter dead cells, while living cells can protect themselves from it...causes it to scatter (FSC/SSC), the detected photons can be represented through a graph of photocurrent vs... under the curve (A).   While flow cytometry can be used for virtually all kinds of experiments and... processing prior to running my flow analysis, I can however clearly identify my lymphocyte population...The voltage describes an electric potential that can be applied to the photomultiplier inside the cytometer...acquisition voltage (not shown) used in my flow analysis I can spot my lymphocyte population distinct from cellular...

...academic researcher at a nonprofit institution, you can order plasmids covering a wide range of fields from...highlighting just a few of the many useful kits you can order from the repository: FX Cloning Kit BIOFAB...information about what these kits contain, how they can be used, and how they’ve been used to date . If you...encoding small leader peptides) downstream of which you can clone your gene of interest. Translation of the standardized...bicistronic design (BCD). The 49 vectors in this kit can be used to drive production of your favorite gene...characterized in Cambray et al., 2013. These terminators can be used to precisely tune your polycistronic E. coli... the epitope tags. Different plasmids in the kit can be used to purify proteins from E. coli, Bacillus...

...Protein can then be purified from the cellular debris. There are several popular DNA vectors that can be used...first be transcribed into messenger RNA (mRNA). mRNA can then be translated at the ribosome into polypeptide...biotechnological, and medical applications. Researches can visualize proteins in vivo by tagging them with fluorescent... structure, interactions and functions. Proteins can also be purified for use in molecular biology research...medicine (e.g. insulin). Proteins, unlike DNA which can be relatively easily synthesized, must be produced...modify your protein of interest. Modified proteins can then be extracted from lysed cells through the use... expression post-transfection. Mammalian systems can express proteins both transiently or through stable...

...panels, as fluorophores that cannot be distinguished using conventional flow can (sometimes) be distinguished...However, emission spectra of certain fluorophores can “spill over” into detectors that are not the primary...the emission spectra of the fluorescent molecules can be quite broad, detectors will usually only pick ...rainbow box” plots are unique to spectral flow and can be thought of as a series of histograms flipped onto...and R4 channels. These unique peaks in the spectra can be used to distinguish these two fluorophores from...markers that needs to be used with spectral flow—you can run a three color panel or a 40 color panel! When...level of fluorescence called autofluorescence (you can see this in your unstained flow controls). Spectral...

...line that can be transfected at high efficiency. Lentiviral transduction Cas9 and gRNA can be present... For CRISPR experiments, the following framework can help get you started: Determine what type of outcome...reporter protein such as GFP? All of these outcomes can be most effectively achieved with different CRISPR...mutations and tags, while a catalytically dead dCas9 can be used in conjunction with activator or repressor...domains to control gene expression. Base editors can help you make precise point mutations near the gRNA...components based on your model system. CRISPR reagents can be delivered via transfection, nucleofection, viral... have identified the best expression system, you can then choose the best method for introducing those...

...experiment can shed light on the mechanism by which cells gain resistance to drugs and can identify future...specific gRNAs can lead to skewed results. Selecting a cell type - Theoretically, any cell type can be used ...Identifying which genes are important for a phenotype can lead to a wealth of additional experiments investigating...sequence within the gRNA is defined by the user, and it can be easily modified to target Cas9 to virtually any...loss-of-function mutation within the target gene. CRISPR can also be used to activate or repress target genes ...genes involved in a particular phenotype. CRISPR can be readily scaled up for genome-wide screening due...-to-use lentiviral preparation from Addgene, you can skip the steps above! Cells are then transduced with...