We narrowed to 511 results for: ATO
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TypeBlog PostPublishedApril 7, 2022, 1:15 p.m....SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging However, the first two generations... 30377363 Aggarwal, Abhi, et al. . “Glutamate indicators with improved activation kinetics and localiation...
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Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog PostPublishedJuly 21, 2020, 1:15 p.m.... at 230 nm. The A260/A280 ratio is used as an indicator of DNA purity. Ideally, this number should be ...like a PCR product). This method also gives an indicator of DNA or RNA contamination based on the presence... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....following up on these leads as we speak, with collaborators in Paris and New York. We want YOU to join the...impact on virus replication. While we and our collaborators are starting these experiments, there's so much... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....relevant science." - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong - and ... Tsang, Science Communications and Marketing Coordinator Non-science favorites The Mistborn series by... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....For my undergraduate degree, I majored in human anatomy, but I also took classes in biochemistry, microbiology...the time I was ready to graduate with my BSc in Anatomy, the University of Otago had established the Centre... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m....bodies do for you? R bodies are robust linear actuators that could be used in a variety of applications...applications, purified R bodies could also perform as actuators in microelectromechanical systems (MEMS) devices... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m...., one that is not food, not medicine, and not mandatory – people can decide for themselves if they want...duty to pursue a degree in a synthetic biology laboratory and received her M.S. from CSU in 2014. Outside... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m.... sequence (“microhomologies”). NHEJ doesn’t obligatorily introduce indels. Given the end structure of...JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F (2013) Double Nicking by RNA-Guided... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....Vellai lab’s new plasmid, T444T, contains T7 terminators adjacent to the T7 promoters to prevent the incorporation...can take as long as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m.... cassette provides increased simplicity for combinatorial genetic screens, and this optimized toolkit ...by the fusion of Cas12f with a transcriptional activator, CasMINI is able to induce high levels of gene... -
Viral Vectors 101: Viral Vector Elements
TypeBlog PostPublishedJuly 25, 2023, 1 p.m....respectively, act as RNA polymerase II promoter and terminator regions and are slightly different in sequence.... Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector ... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m.... Expression Low Tropism for Mouse Cells and Hematopoietic Cells Low Packaging Capacity (~4.5kb From...S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls... -
How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m....technical skills, and experiences from thousands of laboratories that makes up our Help Center, blog, website...graphic designer upon request that the science communicator "just sketch a western blot out quickly." Final... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog PostPublishedFeb. 2, 2021, 2:15 p.m....don’t guess. Simply reach out to the program coordinator with your question(s). This precautionary step...notice within 24 hrs, send a note to the program coordinator. 6. Virtual interview prep Prepare for your... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains contested...counselor, Director of Education for the Jackson Laboratory for Genomic Medicine and a Senior Research Associate... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m.... plasma membrane to be activated by the DREADD actuator after it has been administered. Although we found...Research Center. Using a combination of in vivo and anatomical techniques, her research aims to gain a deeper... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m....interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm... turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving board” so you end... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m.... probably left your plate for too long in the incubator. The small colonies are called satellite colonies...your sequencing results back, you can use the chromatogram to spot whether any discrepancies between your... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....our curated list of biosensors too, as another laboratory may have already created the sensor that you ...PMC18018. 2. Miyawaki, Atsushi, et al. "Fluorescent indicators for Ca2+ based on green fluorescent proteins ... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe (fission...Distribute pending QC. The name of the Principal Investigator and Organization where your plasmids were first... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....human tRNA genes and, until now, we've overlooked regulatory fragments of these transcripts. I remember when...Medicine presented another unexpected finding in the regulatory RNA field. The Rajewsky lab is studying circular... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....open worm browser, which allows you to view the anatomy of worm layer by layer, the NeuroML C. elegans ...neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle tissue as ... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some ...Daniel M. Sapozhnikov is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....ask the depositing laboratory to review the discrepancies. If the depositing laboratory confirms that these... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ...intuitive guide for checking their staining and neuroanatomy without the fear of referencing a wrong section... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....this study laid the foundation for how different anatomical regions of the brain receive inputs and compute.... 5. Nassi JJ, Cepko, CL, Born RT, Beier KT. Neuroanatomy goes viral! Front Neuroanat. 2015 Jul 1;9:80... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....experiments in the beginning. In most classrooms, the laboratory experience is focused on classical embryology...for other developmental models including flies, nematodes, and zebrafish Find plasmids for Mammalian RNAi... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....experiences to undergraduate students (1, 2), but educators face significant challenges in designing suitable...Functional genomics research in an introductory laboratory course. CBE-Life Sci. Educ. 17, ar1. (2018). ...