...It’s clear that CRISPR-Cas9 technology has really changed the game for anyone looking to quickly and ...question. Additionally, a concurrent Nature Biotechnology article describes the development of a mouse...accessible before are now accessible using this technology.” GeCKO library for genomic screens Using lentivirus... Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs...

...sensitive detection of the plant hormones strigolactones By Fabienne Furt Current methods used to detect strigolactones...CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR Corner New ready-to-...intensity, it provides a direct and quantitative detection of SLs. The authors also showed that the receptor-based...

...purification tools (and can have secondary functions as detection aids.) When a single protein is expressed, with...from solution. MBP antibodies are available for detection and/or purification of MBP-tagged proteins. The...alternative to amylose resin and also provides a visual detection mechanism via applications such as western blot...of interest, especially if downstream antibody detection is desired… and yes, we have an antibody for that...

...limitations by using gas vesicle reporters that are detectable by an inexpensive and widely available imaging...fluorescent or bioluminescent reporters usually only detects the signal of the reporter and fail to pinpoint...each ARG’s distinct ultrasound fingerprint can be detected. Bourdeau et al calls this method acoustic collapse... in relation to internal organs. The limit of detection for arg1 expressing ECN is 109 cells/ml, which...

...material for a ChIP-seq experiment can be a major technical hurdle.   ChIP-seq requires  cross-linking during...improvement and simplification of the original technique, specifically in terms of initial steps of cell...ChIP-seq, especially in situations when it is technically challenging to collect large numbers of cells...the CUT&RUN method, it seems likely that this technique will quickly become a standard method alongside...

...Snapgene generated maps provide improved feature detection with an easy to use interface. We regularly use... linear or circular sequence. Next, we use the “Detect Common Features” function to find and annotate ...s “Import primers from a list” function, we can detect all of the primers we have designed that will anneal...plasmid maps and sequences use the same feature detection software as the desktop version of Snapgene. When...

... be your critical experiments and why? What techniques and skills do you need to use to reach these ...address a topic in a mechanistic way using the technology available.” Choosing a research project “entails...entails good judgement with the current technology to identify a problem, asking what approach will I take... importance of combining “new discoveries or technologies increase chances that your project will be fresh...

...AAV viral preps to easily find and share useful technical data. These data focus on experimental design ... These questions are focused on injection and detection methods. For example, you can provide location...you wait until performing the experiment? What detection methods did you use for expression of AAV cargo...Considerations For Precise Neuronal Targeting Using AAV Technologies Using AAV for neuronal tracing Resources on...

...context of IHC, but the problem can impact other techniques like Western blots or immunocytochemistry (ICC... described here may be as effective for other techniques, but it is important to be aware that the issue...Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free Reagents, Immunofluorescence..., Jacob DA (1990) A novel immunohistochemical technique for demonstration of specific binding of human...

...no post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary basepairs...called TOPO-TA) cloning; however, the TOPO cloning technique has also be adapted for blunt end cloning. So ... DNA ends to facilitate replication. The TOPO technique specifically uses Vaccinia virus-isolated topoisomerase...Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ...

...the chance to win some of the latest tools and technologies in life sciences research. The rare disease ...countries. “Over $600,000 worth of cutting-edge technologies were awarded to study 31 rare diseases,” said...ll combine induced pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit...those mutations, they now plan to use CRISPR-Cas9 technology to create cell lines and/or mouse models carrying...

...fluorescent ligands to the loci of interest. Dual color detection In order to label two loci simultaneously, Ma ...CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating MS2 ...target site. Of these, 26 of the 46 loci gave a detectable signal in human U2OS cells. Based upon this, ...engineered sgRNAs using CRISPRainbow.” Nature Biotechnology 34 (2016): 528-530. PubMed PMID: 27088723 PubMed...

...Positive vs Gram Negative. Technology Networks (2019) https://www.technologynetworks.com/immunology/articles/...

...experiments in this publication were performed using a technique called nucleofection, which introduces functional...2016, and Kim et al 2014) have shown that this technique supports extremely high frequencies of genome ...experiments to try nucleofection as (in our hands) this technique supports gene disruption or gene replacement frequencies...CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed PMID: 26789497.  2. Lin, Steven...

...analysis blog post. Colony PCR Colony PCR can detect the presence or absence of DNA using lysed bacterial...plasmid. In this case, you’d be using colony PCR to detect your insert, either by using primers that are specific...time-intensive than Sanger sequencing or the other techniques mentioned above. In molecular biology, cloning...By using a variety of screening and selection techniques, you can increase the chances of finding the ...

...revolutionary physical approaches. However, with each new technical achievement, the complexity of the device increases...PhD, I have been convinced that most microscopy techniques can be replicated with commercially available...available tools and components. Rapid prototyping techniques such as 3D printing, on-demand CNC milling, and... and PCB manufacturing. These newly developed techniques are the key enablers for making assemblies open-source...

...inspire them and also keep up with all the new biotechnology.” These are the types of inquiries that routinely...experiential learning. “Students may forget the technical details they hear in their classes,” she said,...module currently under development comes from the Caltech lab of Professor Richard Murray. Murray’s lab uses... DNA circuits and logic gates. This BioBuilder-Caltech collaboration has resulted in a new BioBuilder ...

...at each locus, as well as large deletions. They detected fragment deletion with 4-20% frequency at the ...you have any questions or thoughts about this technology, leave them in the comments below.  References...plasmids from this publication at Addgene Other Techniques for gRNA Multiplexing Multiplexed and programmable... Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Jun;32(6):569-76. doi: 10.1038/nbt.2908...

... but this skill is not just about reacting to technical issues. This skill also comes into play while ...encounter. As you spend time in a lab learning techniques and taking on independent projects, you will ...solutions. Ultimately, problem solving is less about technical know-how and more about how you approach new challenges...important. Paired with your other skills, both technical and transferable (i.e. teamwork and time management...

.... We also opened up applications to research technicians and undergraduate researchers, in addition to...Abhi Aggarwal is a postbaccalaureate research technician in Kaspar Podgorski’s lab at Janelia Research...variants of iGluSnFR3 in vivo and have used it to detect glutamate release from hundreds of synapses at ...believe that improved biosensors or biosensors that detect new redox metabolites are vital for researchers...

...innovations” stimulates excellence in science and technology by integrating sex and gender analysis into research...anything unique to male stem cells. Nor did they detect important differences in function between male ...international expert on gender in science and technology and currently directs the EU/US Gendered Innovations...Nations on the topic of “Gender, Science, and Technology” in 2011 and again in 2014. Resources on the ...

...to your cloning efforts. Golden Gate cloning technology relies on Type IIS restriction enzymes, first...Voytas groups described a new Golden Gate-based technology for genome editing which allowed for the ordered...efficiently in just a few steps. More recently, CRISPR technology has adapted Golden Gate cloning for inserting...Expression of gRNAs Choosing a Molecular Cloning Technique  ...

...actively pursue. Proposal writing: Improves technical writing and communication skills, Elucidates... the limitations of current state of the art technologies. Therefore, take time to review the literature...insight into what the desirable features of future technologies should offer in order to build upon and address...proposal to test hypotheses and develop novel technologies! If data from a publication or preliminary data...

...presentation as an opportunity to demonstrate technical and transferable skills that make you qualified...perfect science talk Be prepared for potential technical difficulties. Have your presentation on a flash... quiet place to take your video call.  Expect technical difficulties. To try and circumvent this, test...from HR carefully. And even if you don’t have technical challenges, your interviewers might.  You’ll probably...

...that can be tweaked to target ncRNA. CRISPR/Cas9 techniques and targeting methods Knock-out: Targeting short...targeting of long non-coding RNA genes. Nature biotechnology. 2020;38(5):573. doi:10.1038/s41587-020-0428... cells by Cas9 targeting of splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038...Non-Coding RNAs in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant...

...at the Korea Advanced Institute of Science and Technology (KAIST) and Institute for Basic Science (IBS)...primarily to the cell body with very little signal detected along the neurite (Figure 1.) Moreover, the group...Zhang’s lab is developing new super-resolution techniques for live-cell imaging of the dynamic changes ...spatial and temporal organization. The design use to detect PKA activity can be generalized to measure phosphatase...

...Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells remain difficult...using peptide-assisted genome editing. Nature Biotechnology, 10.1038/s41587-023-01756-1. https://doi.org...monomeric StayGold fluorescent protein. Nature Biotechnology, 1–4. https://doi.org/10.1038/s41587-023-02018...throughout the non-human primate brain. Nature Nanotechnology, 18, 1241–1251 (2023). https://doi.org/10.1038...

...Brain Research Through Advancing Innovative Neurotechnologies (BRAIN) Initiative Cell Census Network, the...purposes. The Wu lab mostly uses whole-mount IHC techniques on rodent brains and is expanding to more complex...antibodies (See Fig 1.). rAbs are a relatively new technology that takes advantage of DNA sequencing to turn...antibodies. Since recombinant antibodies are a newer technology, it is important to show through qualitative ...

...molecular biologic techniques to further the understanding of epigenetics; techniques include chromatin...and bisulfite sequencing. However none of these techniques can be used for in-situ research of dynamic modifications...reporting methylation status of these sites. This new technology opens up a new way to visualize changes in gene...

...variety of research sectors to provide open source technologies and spaces where interested parties can do research...fantastic optics and fluidics platform it contains. Biotech equipment becomes obsolete amazingly fast, but ...is a passion for learning and having fun with technology, so feel free to contact your local space and...Biocurious.org, Derek is bringing cutting edge technologies to Victoria, BC. Follow Derek on Twitter @derekja...