We narrowed to 662 results for: Clu

...Volunteer at a local high school science class: Science Club for Girls) Communication to diverse audiences...Communication Business models Conference or club organizer Management Leadership Communication...

...understand and grow the scientific ecosystem, including understanding how shared materials are used to...ll be confident in what to expect. No system, including MTAs, is perfect, but it does provide consistency...

...are multiple methods of DNA assembly available, including: Gibson Assembly®, BioBrick® Assembly, Golden ...applications of the NEBuilder HiFi DNA Assembly Mix include:  Generation of constructs with multiple point...

...oligomerization of the Dronpa domains thereby occluding the enzyme active site and shutting off the enzyme...of interest into its component simple sugars (including lactose), and a lac operon controlled GFP. When...

...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...libraries. NEB also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated...

..., across four different types of human cells, including T cells and hematopoietic stem and progenitor ... from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely on an upstream...

... browse our entire AAV inventory. Our new AAVs include: Control AAVs for serotype testing jGCaMP7c for...best serotype for their particular study, which includes comparing the transduction patterns of different...

...pivotal role in an array of biological processes including development, the immune system, and cell turnover...been difficult until now (Zhang et al., 2019). Conclusion FlipGFP is a novel fluorogenic reporter that ...

...of physical materials that we use in our labs, including plasmids. All plasmids at Addgene have unique ...materials and methods section have been updated to include the option of citing with resolvable compact identifier...

...researchers explore many aspects of ApoB-LP biology, including quantification of abundance, size, and localization...made these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as the eFRET sensors...

... new contact to connect on LinkedIn and always include a personal note about where you met and what you...can be as simple as “it was nice meeting you.” Include follow-up questions or requests for advice based...

...that can influence the HDR/NHEJ decision. Our conclusions reinforce a key scientific principle: that understanding...frequencies of genome cutting and, when donor DNA is included in the nucleofection reaction, extremely high ...

...plasmid on the right has a total size of 7.3kb, including a 1.2 kb insert. The plasmid was digested with...run on an agarose gel. The resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from...

...plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is...in the cell, as many of these experiments are concluded within 48 h of transfection. Of course, it is ...

... is not fluorescence! Chemiluminescence, which includes all bioluminescence, is the production of light...Promega to offer their Promega Plasmid Collection, including tools using NanoLuc®, NanoBiT, and more. Which...

...most likely to be clustered at signals below 103, while the GFP+ population cluster would be shifted to...

...genome. Typically, it is the sequences between and including the LTRs that are integrated into the host genome...internal promoter. An internal promoter must be included as the wildtype LTR promoter is no longer present...

...shares many common features with gamma-retrovirus including genome architecture, host integration, and its...its cargo, in fact its viral life cycle does not include genomic integration. While the expression of adenovirus...

... tools with many applications. These libraries include sgRNAs targeting the entire genome, overexpression...several advantages over traditional vaccines, including 1) cellular responses in addition to antibody ...

...Reis e Sousa from the Francis Crick Institute concluded “Aspirin promotes autophagy, inhibits platelet...field of Immuno-Oncology, a sizzling hot topic, includes studies on novel cancer therapeutics such as  ...

...information on each cancer clinical sequencing report includes rare variants that haven’t been characterized?...the scientific community through Addgene. These include 634 sequence confirmed mutant cDNA clones corresponding...

...expression of other endonucleases, like Csy4, or the inclusion of processing signals, such at tRNAs, to process...its own pre-crRNA arrays eliminates the need to include multiple promoters to drive crRNA expression and...

...will it make them think? Does this lead to the conclusions I want them to draw and the perspective I want...scientists has formed around science communication, including the teams at Addgene, ComSciCon, and many more...

...Other examples of chemically induced promoters include positive inducible alcohol and steroid regulated... be induced by heat or cold exposure. Examples include the heat shock-inducible Hsp70 or Hsp90-derived...

...sociology, behavior, and artificial intelligence (including a team that created a novel form of AI that can...require large data sets. Whether these data sets include videos of behavior, many many images of brain regions...

...backbone to generate R-mAbs of different subclasses, including subclasses that were not produced by the original...expanded their recombinant affinity reagent tools to include nanobodies. At Addgene, we are excited to be part...

...Others ran experiments at odd hours, used more secluded workspaces, or politely asked to not be disturbed... of making an error. Try a variety of things, including (and maybe especially) suggestions you are initially...

...generate antibodies against many types of molecules including proteins, lipids, and carbohydrates, in this blog...2005). This is reflected in the antibody’s cost. Conclusion Whether you choose to use a monoclonal antibody...

...organisms in which it was previously not an option, including Drosophila and C. elegans.   Find the TurboID ...that one might perform using the purified domains include protein stability, small molecule binding screens...

...80,000+ reagents and 700+ educational resources — including blog posts, protocols, guides, and collections...we rolled out multiple changes to our website, including the recent homepage and search tool updates. Now...

...be activated by an array of different ligands, including hormones, neurotransmitters, photons or odorants... new receptors were termed Designer Receptor Exclusively Activated by Designer Drugs (DREADDs) (Roth, ...

... DNA there, although other restriction sites (including other XbaI sites not preceded by GA or followed...either be difficult to interpret or lead you to conclude your plasmid is not correct! Conversely, enzymes...

...intact β-galactosidase produces pigment from x-gal (included in the transformation plate medium), turning the... colony blue. We should note that IPTG is also included in the medium to ensure transcription of the lac...

...other required parameters of the coupling system include: Wide fusion-tolerance — Click Biology tags should...HaloTag Collection of research tools from Promega, including many HaloTag plasmids Exchangeable HaloTag Ligands...

...we’ll be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think...growing library of antibody educational resources, including our new Antibodies 101 eBook, the Antibody Guide...

...Bornhorst 2000). To prevent this, wash steps often include low concentrations of imidazole. The concentration... C-terminus and how many histidine residues to include.  Placement As far as placement goes, there is ...

...published, a bioRxiv preprint that questions the conclusion of inter-homologue recombination was released...off-target effects attributable to CRISPR editing. Conclusions & implications One striking feature of this paper...

...photobleaching rate is not linearly related to power, the conclusions reached at high power may not translate to real...cells at a power used for live-cell imaging. To conclude, the most photostable fluorescent protein can ...

...proteins that are found in and on everything, including your skin. Here are some general tips for working...degrade RNA. Keep a clean work area, which may include spraying your bench down with a product to get ...

...recognized for its biotechnological applications including conversion of CO2 into water and electrical energy... in cyanobacteria. This kit contains 96 parts including terminators and promoters, CRISPRi parts, selectable...