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We narrowed to 795 results for: Kin

Showing: 281 - 320 of 795 results

  1. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...expression cassettes (Shaw et al., 2023). Those looking to express high levels of their gene of interest...more! Engineering disease resistance through gene stacking, increasing production of a protein of interest...requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities really are endless...mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was designed to work in....2024.100519. Chiasson, David, et al. “A Unified Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports...

  2. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ... don’t want to spend months doing surgeries and making fiber optic cables only to hook up your lasers ...alter the light spectrum for activation and the kinetics of opsin activity/inactivity. Dr. Deisseroth has...(not expressing the opsin) are also available. Picking your opsin  You must consider whether you wish ...neuronal firing, the cell-type specificity, the kinetics of the opsin variant, and other particularities...the promoter of the cre recombinase while still taking advantage of the robust expression via the promoter...

  3. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...Francisco International Airport listening to the Lion King soundtrack and writing this post, it is my pleasure...what new post contributed the most to this record breaking month and to find other posts that deserve a second...Navigate a Scientific Enterprise in Flux CRISPR Kinome Libraries Available: Pooled and Individual Plasmid... Research Community Grad School Advice Part 1: Picking a Lab and a Project That’s it for now. As I indicated...

  4. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Tools for Making Scientific Graphics by Beth Kenkel Find a round-up of tools you can use for making scientific...we're gearing up to start a new decade, we’re also looking back at the last year of scientific discovery. ...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim Goedhart Guest...

  5. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    Published
    Jan. 10, 2019, 2:38 p.m.
    ...at a lindy hop swing dance event,” Zheng says (networking can happen anywhere!). The pair quickly teamed... in November 2017. #Phage researchers! I am working with a team to get Burkholderia cepacia phages ...must recognize a specific part on the bacterium making each phage is very specific. Some infect only one...about scientific sharing Find blog posts about networking Additional resources on Addgene.org Find molecular...

  6. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...excited to finally share with you what we’ve been working on behind the scenes here at Addgene.  Addgene's...messaging, as well as simplifying the page, and making it all look clean and easy to use.  Easily search...global navigation and menus over the past year, making it much easier to get around our site.  Community...smile, but occasionally you might notice Blugene looking sad, especially if an experiment doesn’t go as ...

  7. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...experiment. (And you’re certainly not alone – we’re all looking for high-quality antibodies and reagents that work...and sorting. Reference images can be viewed by clicking on the links in the "Agree" and "Disagree" columns...associated metadata adapted from the Affinity Reagent Working Group of the Human Biomolecular Atlas Program (...community when designing the IBEX Knowledge-Base, seeking to create an iterative process that is constantly...

  8. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ... search. This is especially true of scientists seeking a first job after the academic bench. Utilizing... the job posting instructs even if you have a networking connection that will be helping (see below). ...strengths in your resume and don't be shy about asking for help. The worst thing that can happen is someone... scientist networking...

  9. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...,” said Addgene’s Lianna Swanson, who has been working with members of Davidson's lab to coordinate the...For more information on choosing a backbone and making fusions, see this blog post from Joachim Goedhart...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many... many more! Baird has a helpful tip for anyone looking to peruse the collection without getting overwhelmed...

  10. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics and allow...set of tools therefore depends on what you are looking to study. Figure 1: A schematic showing the...receptors in the brain. One way to address this is by working with organisms with light accessible brains, such...

  11. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...paper they’re reading has been linked in a ReFigure making it easy for others to discover your insights on...researcher Christa Osuna collated Zika case studies, making a meta analysis available to the community on ReFigure...PubMed Central, eLife, PLOS and Figshare but we are working on expanding our reach. We can’t save you the hard...career researcher and cofounded ReFigure with Joe Akin. She envisions a world where the impact of teaching...

  12. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...your plasmids are distributed. Next month, we are making two changes to the policy that will make the process...the plasmids online for distribution instead of risking leaving them on hold indefinitely. Of course, the...citation and release the hold. Increasing impact Making the plasmids available as soon as your paper is... paper comes out later. Or, if a project isn’t working out as planned, but you still hope somebody will...

  13. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...and you are smart, you can be a good manager. The kind of smarts and the type of skills that it takes to... from being included in organizational decision-making. It is imperative that employees are thanked for...manager keeps these factors in mind at all times when making decisions, assignments and polices. Sometimes the...priorities for advancement in science Have their own working schedules and may not necessarily be comfortable...

  14. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...persuing milestones and working hard. Being patient, being selective, and talking to peers and mentors will...with the current technology to identify a problem, asking what approach will I take and what can I learn,... Dr. George Church (Harvard Medical School) for taking the time to speak with us about adding new scientists...

  15. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...inclination to be a teacher was looking for an outlet. I started asking people: why do you do what you ...bioscience into context, while at the same time working through the details of experimental design and ...of science communication professionals who are thinking about, for example, how to use new technologies...

  16. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...whirlwind of skill acquisition. Classes covered filmmaking, podcasting, philosophy of science, creative ...life of Srinivasa Ramanujan, to be precise) and remarking to a classmate that, if this was one day required...communication is an equally fruitful endeavor for those embarking on the route towards academia. Many of those entering...master's degree in 2020, his research explores risk-taking behavior among collision sport athletes, as well...

  17. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...DNA yield. Test multiple, small colonies After streaking LB agar plates, you may notice that there are ...recombined product tend to grow faster, so we recommend picking the smaller colonies which are more likely to carry...the recombined vector backbone, you can try re-streaking for single colonies and testing multiple preps...

  18. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...immunohistochemistry images that are crisp, bright, and lacking in non-specific staining can be a challenge in ... mixing your primary and secondary antibodies, blocking excess secondary with serum from the same species...problem.  Pre-block with Fab fragments (Figure 2C) Speaking of Fab fragments… Another strategy for species-on-species...0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody...

  19. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...just on the disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they...proteins. This is, for the intrepid researcher looking for molecular biology tools, a very useful trait...Created using biorender.com   If you’re new to talking about viruses, here’s a quick tip on terminology...reproduce only in experimentally controlled conditions, making them far safer to work with than virions. The four...

  20. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...features provide extra layers of risk management when working with viral vectors. Typically, one day after transfection...particles are secreted into the cellular media, making viral collection fairly easy. Producer cell media...transfect’ cells lines are amenable to transduction, making it a logical choice for efficiency. Where viruses...with alternative methods. Ready, set, transduce! Making virus can sound daunting, but it is easier than...

  21. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic factors that ...and we’ve been using "plasmid" ever since! From napkins to notebooks Although discovered in the early 1950s...initiated at a Hawaiian deli in 1972. Using a deli napkin for paper, a small group of scientists including... curate, and distribute them all in the name of making it a little bit easier for scientists to conduct...

  22. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...amplification looks like, but by looking at these kinds of graphs, making sure few/no plasmids have dropped... the key points you’ll need to remember when undertaking library amplification and sequencing. Maintain...

  23. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...Will the job require public speaking? Will you be leading a group or working as part of team? Will you .... It is all too easy to get tunnel vision when working towards a specific goal, and when you realise that...often be misleading. Key aspects to determine are working hours (9am-5pm Mon-Fri or overtime and weekends...

  24. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...established in yeast, including a widely used Parkinson’s experimental model. In fact, approximately 25%...phaffii is highly efficient at protein secretion, making harvesting recombinant proteins a cinch. This is...it doesn’t secrete its own endogenous proteins, making the recombinant protein its major secretion. Beyond...players in the game (even beyond K. phaffii ) worth looking into whether you’re just dipping your toes into...

  25. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...will define positive selection as the means of picking up positive traits (i.e. the plasmid contains a...toxin), whereas negative selection would be the picking up of a negative trait (i.e. the plasmid contains...Conferring Resistance Cell Types* Mode of Action** Working Concentation*** Blasticidin bsd  HeLa, NIH3T3...transfection when creating stable cell lines. Looking for Mammalian Expression Plasmids? Check Out Addgene's...

  26. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ... with translatable skills: working with data, working with people, working with complex processes Arm ...Ginkgo Bioworks, our software development team was working on lab operations software for next-gen sequencing...

  27. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...spread of COVID-19. That’s why we’re proud to be working with Ginkgo Bioworks to synthesize and share over...analyzed first thing the following day. Nothing like waking up to fresh data! These same plasmids are then ...ensuring our rigorous QC standards are met, we are taking a unique hybrid approach compared to our standard...high throughput platforms, Addgene and Ginkgo are striking a balance by modifying certain processes in both...

  28. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify...components: the error prone PolI3M that’s fused to a nicking Cas9 (nCas9). Similar to mutagenesis with error...error-prone polymerase (PolI) that is fused to a nicking Cas9. A guide directs Cas9 to a site of interest...changes promote nCas9’s dissociation from DNA after nicking and yielded an enhance nCas9 (enCas9) EvolvR with...

  29. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...work best for your scientific question? If you’re thinking, ‘wait, those terms mean different things?!’, ... to clearly define what type of factor you are looking for and what process or outcome that factor functions... advantage – that is, you are not specifically looking at cells that can survive. Instead, all manipulations... screens are similar to CRISPR, but instead of knocking out the genes of interest (or the whole genome...

  30. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ... the experiment in my head, double-checking details, or just taking a moment to relax. During really stressful...worth doing, and doing well, so make sure you’re taking time to do them carefully and thoroughly. Have ...however, many ways for you to reduce the chances of making an error. Try a variety of things, including (and...

  31. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...gate which selects anything to the right of it, marking it as GFP+. Quantification for such graphs could...plot! The dreaded smear - fear not! When you are looking to study the level of protein expression, instead...Figure 2). These smears tend to occur when you are looking at proteins that are expressed regularly in cells...percentage positive or negative in your dot plot, looking at a histogram vs antibody plot would be better...

  32. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...question more and more students are asking. Teachers are also asking, “How do I engage my students in real-world...outcomes by emphasizing the importance of critical thinking, persistence, teamwork, and communication. And... the only informal synthetic biology club of its kind, running from start to finish during the academic...

  33. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Ku then acts as a scaffold for recruitment of a kinase (DNA-PKcs) and a two subunit DNA ligase (XRCC4-...heterogeneous when constrained by sequence identities in flanking sequence (“microhomologies”). NHEJ doesn’t obligatorily...Renouf B, Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson EA, Giovannangeli C, Jasin M, ...Inoue A, Matoba S, Zhang Y, Zhang F (2013) Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing...

  34. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...Developmental Editor at F1000Research.  With many changes taking place in the world of science publishing, and an...include in my paper? More and more journals are asking for detailed information on lab resources, such...enough information to replicate your experiments, making research more reproducible and your results more...journals as possible at the same time. Take your time picking the appropriate journal and explain in your cover...

  35. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...note: in this table, reprogramming efficiencies ranking as low, medium or high is based on the reprogramming... pluripotency of mouse cells typically involves making chimeras by injecting iPSCs into blastocysts and... injecting iPSCs into immunodeficient mice and looking for tumors that contains cells from all three germ...of iPSCs is the number one priority, with safety taking lower priority. Genomic integration is less of ... and Juan Carlos Izpisúa Belmonte. "Methods for making induced pluripotent stem cells: reprogramming à...

  36. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...promote tumor durability instead of repressing them, making it even harder to cure cancer. To understand how...factor, vascular endothelial growth factor, and interleukin-6 (Witkiewicz et al., 2009) which contribute ...metastasis of tumors through inciting inflammation. Speaking of inflammation, a noteworthy cell type in the...Witz, Isaac P. "The tumor microenvironment: the making of a paradigm." Cancer Microenvironment 2.1 (2009...

  37. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...like cross-linking immunoprecipitation (CLIP) identify RNA-protein interactions by cross-linking RNAs and...enrichment compared to E. coli’s BirA*. Faster labeling kinetics also means that BASU could potentially be used...RaPID RaPID sounds like a nifty technique, but what kinds of questions can it help answer? Ramanathan et al...

  38. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...with a heterozygous 4 bp deletion in MYBPC3 donated skin, blood and semen samples. Ma et al. designed plasmids...of only 27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells. The results were...CRISPR editing. Conclusions & implications One striking feature of this paper is the failure of the embryos...comprised of individuals with varied backgrounds. Talking about this study with non-biologist friends Like...

  39. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...autophagosomal membranes, so tagging it with GFP allows for tracking the localization or accumulation of pre-autophagosomal...an autophagy-independent manner. Additionally, tracking Atg8p or LC3 doesn’t provide information about...easier to study the mechanisms behind autophagy by tracking what’s being transported to the yeast vacuole ...vacuole (i.e. cytosol, mitochondria), rather than tracking generic autophagy markers. It’s particularly useful...

  40. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...the design of flanking overhangs. Although there are theoretically 256 distinct flanking sequences, sequences...their recognition sequence, creating four base flanking overhangs. Since these overhangs are not part ...clone up to 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages of Golden Gate...
Showing: 281 - 320 of 795 results