We narrowed to 932 results for: Gene

...which NANOG-related genes are critical to stem cell survival. Knowing which genes influence the survival...compaction, an enhancer can increase the expression of a gene that is millions of base pairs away. (For details...GPS for the genome” to compute coordinates for genetic elements like protein, RNA, and DNA (Table 1). ...family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...differentiate over time, we periodically use next-generation sequencing to measure the relative proportion...pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing your own CRISPR...Hypersensitive Sites and Intergenic CRISPRs Yours truly generated the DHS data set by starting with all of the DHS...

...versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also discussed in the...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's addition to the poster session was a first...attendant called them, "long, skinny things") and generally exuding a very-tired-but-very-excited attitude...happens): Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges...That so many scientists are tackling this type of genetic engineering is a testament to how amazing the downstream...from the custom software used to design complex genetic circuitry to the downstream output. Tuesday morning's...

...contacting Addgene can be found on our Contact page. You’ve called. You’ve emailed. And Addgene has been...Plasmid sharing in Europe: Addgene’s request statistics Since 2004, Addgene has shipped more than 75,000...into the Addgene repository. More than 4,500 plasmids have already been deposited at Addgene by over 250... your service: Addgene’s London office Just a few weeks ago I became the newest Addgene Outreach Scientist...UPDATE - As of January 2020, Addgene's Europe office no longer handles customer support inquiries. The...Cambridge, MA to be exact. Today, that changes. Today, Addgene launches a brand new Europe office located in London...poised and ready to answer your questions about Addgene, plasmids, and depositing (all while our US counterparts...

...frequently requested. Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was...expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs - Genome engineering methods improved...Addgene was founded 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids...biosensors, optogenetics, and other imaging tools. The GCaMP5 and GCaMP6 series of genetically encoded calcium...Top 10 plasmid technologies distributed through Addgene over our first 10 years. 10 most distributed plasmid...it's not actually a technology, but pSV-PGC1 was Addgene's first distributed plasmid and was created in the...repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid...

...When I got the job of Outreach Scientist at Addgene, my first thought was “I get to talk to scientists... spend a lot of time telling scientists about Addgene’s repository, and explaining how to deposit and ... 1,600+ labs around the world!) that have kept Addgene at the forefront of life sciences research for ...from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and get practical...scientific sharing and the open-access movement. At Addgene, we believe that science gets better when everyone... to tell? Let us know by emailing us at blog@addgene.org or tell us on Twitter and Facebook. We’ll track...

...international researcher who uses plasmids containing genes from rabies and vesicular stomatitis virus, you ... export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster...ordering plasmids, please feel free to email help@addgene.org. We’ll be happy to help! A quick note: While ...complex and may change suddenly. Please email help@addgene.org for the most-up-to-date information on shipping...

...Projects in Wei Leong Chew's lab. Genome engineering, gene delivery methods, AAVs, and more. Chapter 7/Outro... in the areas of genome editing, immunology, and gene therapy.   Special thanks to our sound engineer/...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...Additional Resources from the Addgene Blog Visit our CRISPR featured topic page Check out our Hot Plasmids...synthetic biology researcher Tom Ellis Resources at Addgene.org Find CRISPR Plasmids Find Synthetic Biology ... addgene podcast...

...Hybrid Serotypes: Improved Vectors for Gene Delivery. Curr Gene Ther 5(3), 299–310. PubMed PMID: 15975007...vectors could also be powerful tools for the field of gene therapy if they can reach the affected part of the...anterograde and retrograde axonal transport. Hum Gene Ther 25, 705–720. PubMed PMID: 24694006. PubMed ...2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems...., and Nakahara, K. (2011). Local and retrograde gene transfer into primate neuronal pathways via adeno-associated...-associated viral vectors is serotype-dependent. Gene Ther 20(3), 348–352. PubMed PMID: 22418061. PubMed...vector serotypes 1, 2, 5, 7 and 8 in mouse brain. Hum Gene Ther 18, 195–206. PubMed PMID: 17343566. Tervo, ...

...sequence to confirm backbone elements.   Confirming the gene/insert by aligning to NCBI entry or using BLAST....available reference sequence or plasmids containing genes with many modifications. We look for point mutations...amino acid. We also confirm that the species of the gene matches the data associated with the plasmid.   ...Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum...for all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for quality control...control for over ten years. Now, thanks to a generous donation by the Hirsh family, we are able to take...provide requesting scientists high quality data! Addgene's new in-house NGS process To start, the process...

...control expression of their genes of interest. While recombinases are powerful gene expression tools in and...CRISPR knockout library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the ...blue-light inducible Cre for precise control of bacterial gene expression By Meghan Rego Recombinases are enzymes...precisely controlled through the use of optogenetics. Optogenetic tools use light to induce conformational...Synthetic Biology publication, Mary Dunlop’s lab generated a photosensitive Cre, Opto-Cre-Vvd, by splitting...from Biggiephage can eliminate competing mobile genetic elements. Image by Basem Al-Shayeb in the Doudna...unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed a fusion...

...data Do you use Addgene AAV tools and want to join the ranks of our growing group of Addgene AAV data sharing...treatment conditions like drugs (Chemogenetics) or light (Optogenetics) to activate receptors? If you’re... way to celebrate open data sharing than with Addgene’s AAV Data Hub! We launched this pilot in 2019 as... an open science platform for scientists using Addgene AAV viral preps to easily find and share useful...animal strain used). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over...sharing champions? If you’ve recently used Addgene AAV tools, we invite you to contribute your data to ... for sharing on the site. The entry form has 5 general question types and it takes approximately 15 minutes...

...All plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly...we know biological resource centers, including Addgene, positively affect scientific research. As Maria...Maria Castro of University of Michigan says, “Addgene has transformed the way in which we share plasmids...tool is so influential that it is requested from Addgene many times as the technology disseminates throughout...researchers who have at least one plasmid deposited at Addgene that has been distributed more than 100 times. ... honor for our powerhouse depositors who have generously shared their reagents freely with the research... on sharing because, as UNC’s Bryan Roth says, Addgene has “literally enabled sharing with 100's of investigators...

...that, when you cite a plasmid from Addgene using its unique Addgene ID number, your publication will be...Feature Browse More Inside Addgene Posts Additional Resources on Addgene.org Browse Our Special Collections...plasmid technologies contributed by our depositors, Addgene strives to make it easier for you to find the plasmids...sophisticated search, and curating plasmid collections. Addgene's "How to cite this plasmid" section Anytime you...repository, we request that you cite it using both its Addgene ID number as well as the reference for its original... process, we explain how to cite plasmids from Addgene right on our plasmid pages and provide a citation...Why do this? Not only does citing plasmids from Addgene help others find them later if they need to use...

...with this "Citing Article" - such as cloning their gene of interest into a previously deposited vector -...Plasmid" list. Email us at help@addgene.org with the Plasmid Name, Addgene Plasmid ID #, and the PubMed ...ICYMI: Addgene’s Plasmids 101 eBook Protocols and Plasmid References @Addgene  ...Exciting news! Addgene recently rolled out a new feature on our plasmid pages - links to articles citing...systems it has been validated in.  If a plasmid's Addgene ID # has been referenced in other publications,... see where they've been cited. Benefits to the Addgene community of scientists Benefits to depositing ...Properly citing plasmids you've requested from Addgene may get your paper listed in the "Articles Citing...

...sharing Addgene is all about helping scientists share between labs, and people work hard at Addgene to make...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ...as one of their favorite parts about working at Addgene. We’re a small non-profit, so we can only provide... started Hack Days. During Hack Days, teams at Addgene will forgo their regular To-Do lists (other than...Cross-team “Random Lunches” With more employees, Addgene has naturally divided into smaller functional teams...a year, she’ll mix up the names of everyone at Addgene, and send random sets of 4 people out to lunch.... to gather around a large table to eat with my Addgene family every day. Do you have any traditions that...

...and topology, and cloning and gene expression. Just released: Addgene's Plasmids 101 (2nd Ed.) eBook... as everything from parasites, to symbionts, to genes and the labels applied to them were vague or contradictory...biology was never the same. Over the next few years genes from different bacterial (and eventually mammalian...Bioblasts? Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic...of "cytoplasm" and "id" (Latin for 'it'), as “a generic term for any extrachromsomal hereditary determinant...separate term, “episome”, defined as “a non-essential genetic element which could exist either autonomously or...molecular biologists wanting to study bacterial genetics. This all changed thanks, in part, to a collaboration...

...many of which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested...means researchers who use VSV-G can deliver their genes of interest to a variety of cells and study their...plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours ... articles cite the use of pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad...searchable list here). Tips for using pMD2.G At Addgene, we use pMD2.G for producing our ready-to-use lentivirus...Lentiviral Transduction Additional Resources on Addgene.org Check out our Viral Service Learn more about...

...on the Addgene blog Learn more about plasmid sharing during COVID-19 Resources on Addgene.org Find ...examine a dataset of Liu lab plasmid requests from Addgene, as a proxy for global activity in our particular...over 6 years for 35 papers (data was provided by Addgene on 5/26/20 and contains requests from 5/22/14 to...The inferred weekend effect is, in retrospect, generally more dramatic than the COVID-19 effect, though...not a causal interpretation -- the effect would generally not be the same if weekends were somehow imposed...evolution to illuminate biology and enable next-generation therapeutics. Prime editing, base editing, PACE...

...academia and industry. As a result over 90% of Addgene’s COVID-19 resources are now available to scientists...these groups were willing and able to listen to Addgene’s new approach of working with the industry-based...in the background became a normal occurrence as Addgene and our university partners quickly assessed how...allowed them to synthesize new plasmids quickly and Addgene’s quality control and archiving system allowed us...COVID-19 plasmid collection Beyond COVID-19: Addgene’s plasmid collections available to industry scientists... this alone. If you have deposited plasmids at Addgene and would like to make them available to industry...industry, please reach out at techtransfer@addgene.org and we will initiate a conversation with you and your...

...    This is just the first of many milestones Addgene is poised to reach in the near future! We’re almost...April 8, 2021) Find these stats and more on the addgene.org homepage! You can track the number of plasmids...

...resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe...Subscribe to the Addgene blog Resources on Addgene.org Meet the other members of Addgene's Board of Directors... did you first hear about Addgene? How did you become involved with Addgene? Katz: Joanne and I first ...connection that got to know more about Addgene and heard that Addgene was looking for new board members. ... that Addgene provides the scientific community? Katz: The most important resource that Addgene provides...What do you hope Addgene will accomplish in the near future? Katz: I’m excited that Addgene is continuing... through Addgene’s sharing platform. Expanding further beyond the US and increasing Addgene’s international...

...features of Addgene's website Resources on Addgene.org Try out the new search on Addgene's homepage ...Did you see Blugene’s make-over and our new homepage? If you've visited Addgene’s homepage recently, ...been working on behind the scenes here at Addgene.  Addgene's new homepage While users are increasingly...emphasize the values that make Addgene unique: community, sharing, and fun. The Addgene repository is built on....  Blugene gets a make-over You may also have noticed another big update on the homepage. Blugene, our... ways, we realized that Blugene needed the same thoughtful treatment. Blugene has been with us since 2011...might notice Blugene looking sad, especially if an experiment doesn’t go as planned. Blugene also has full...

...For example, this is an experiment using the chemogenetic tool hM4D(Gi) driven by a GFAP promoter in rat... rat. Contribute your AAV data Just like Addgene’s plasmid repository, the AAV Data Hub is a community-driven...only accepting data for materials distributed by Addgene.  Future directions As the AAV Data Hub grows, ...accelerate your research. Sincerely, Melina & The Addgene Team ...

...Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important for scientific...distribution and disseminate knowledge around the globe. Addgene’s progress in international distribution As you ...are making great progress toward spreading the Addgene community into developing regions like South America...the countries that have requested plasmids from Addgene since our inception in 2004. In the near future...SeedingLabs or on Facebook Additional Resources on the Addgene Blog Learn About Our Recent Visit to China Read...People at Scientific Conferences Resources on Addgene.org Read about Global Plasmid Distribution Check... Institutions that Deposit their Plasmids with Addgene ...

...including: generating single or multi-gene knockouts in a wide variety of cell types, gene editing using...efficiency, requires specialized equipment, such as a gene gun. Depending on the nature of the experiment, ... CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing." Journal of the American Chemical Society...using homology directed repair (HDR), and generating large genomic deletions. What are the advantages of...and specificity is a concern, such as knockout generation or homologous recombination.  However, experiments...step in this type of CRISPR experiment is the generation of the RNP complex.  While one option is to purchase...in vitro transcribed from ssDNA, which can be generated by commercial vendors such as IDT. These two components...

...overexpress genes of interest; more recently we have also been using Addgene CRISPR vectors to generate cell ...cell lines with knockouts of specific genes. Many years ago, people in the laboratory became frustrated with...cDNA is co-transfected at a favorable ratio to the gene of interest (or, if your protein is fused with a...with different levels of expression of our gene of interest. Advantages of the agarose cloning method ...figure 1.   Find Additional Useful Protocols on Addgene's Protocol Pages Agarose cloning method procedure...-well cell plate to a 96-well assay plate. We generally freeze at least 2 ampules each of 4 clones with...som.umaryland.edu.   Additional Resources on the Addgene Blog Tips for a 1st Time CRISPR User (by a 1st...

...contain a toxin gene (regulated by a rhamnose-inducible promoter) and a chromoprotein gene. Allelic exchange...the plate. For example, if your plasmid contains a gene encoding a blue chromoprotein, after transforming...3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus et al., 2019. One such lab is...protein family. What sets them apart is the way they generate their color: they strongly absorb visible light...difficult to directly use in bacteria, where most genetic manipulations start. Anthony Forster’s lab used...recombination events: The plasmid carrying the desired genetic modification integrates into the genome (Figure...art, let us know! Additional resources on the Addgene blog Find our Fluorescent Protein 101 blog series...

...qPCR is used in a variety of applications including gene expression profiling, studying copy number variation... is also used in high throughput SNP genotyping, gene deletion analysis, and RNA detection.  A summary...starting template DNA concentration Gene expression profiling Copy number variations Quantify RNA levels...Disease or pathogen identification SNP genotyping Gene deletion Analysis RNA detection     References...in colony PCR, or for generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase...101: Colony PCR Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase Chain ...concentration of the original template DNA in the sample.  Addgene currently uses ddPCR for AAV titrating. You can...

... the world, it can be hard to find your favorite gene, promoter, serotype, or a myriad of other things...Retroviral) Species: Specify the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid Type: Choose...to find plasmids matching your search terms with gene and guide RNA / shRNA inserts Resistance Marker:...Resistance: Use this to choose the antibiotic resistance gene(s) used for selection in bacteria. Has Viral Service...terms in an Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...Additional resources on the Addgene blog Learn more about other features on Addgene's website ...search, we’ve also revamped our homepage and mascot Blugene (check back on December 5 to learn more about this...

....1216880109  Resources on Addgene.org CRISPR Plasmids – Protein Tagging CRISPaint Gene Tagging Plasmids 101...Resources on the Addgene blog Plasmids 101: Protein Tags Plasmids for Endogenous Gene Tagging in Human...kDa up to ~35 kDa and are typically knocked-in via gene targeting or cloned in-frame to the protein of interest... protease is situated between the degron and the gene of interest and the degron domain is terminal. Between...sequence (7 kDa). This system also requires less genetic engineering than some of the other systems reviewed... leakiness associated with the system, but the general concept of the system has remained the same. An...

...expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target multiple genes in a cell population...’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook...CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease, or both. Applications...

...Utilization During Gene Targeting with Zinc-Finger Nucleases. G3 Genes Genomes Genetics, 3(4), 657–664. ...Jaenisch, R. (2013). One-Step generation of mice carrying mutations in multiple genes by CRISPR/CAS-Mediated ...example, choosing target sites that span the knock-in gene. For making mutations, one good way is to mutate...be okay if you are introducing a selection marker gene. The homology arms should be no more than 100 bp...for my genomic PCR, but couldn't amplify the EMX1 gene using same primer you used in the Science paper ...Winderbaum, J., Nickoloff, J. A., & Jasin, M. (1998). Gene Conversion Tracts from Double-Strand Break Repair...Additional resources on Addgene.org CRISPR guide CRISPR protocols by Addgene depositors gRNA design software...

... to the Hot Plasmids episode! Expanding the optogenetics toolbox with CRY2clust Article contributed by...Basic Science (IBS) has developed another useful optogenetic tool that enables robust and efficient oligomerization...CRY2clust, when integrated into a Ca2+ -modulating optogenetic system (OptoSTIM1), caused two-fold faster changes...soCoChR: A novel opsin that enables single-cell optogenetics Article contributed by Meghan Rego  Listen ...Listen to the soCoChR podcast segment Optogenetic stimulation is a powerful tool used by neuroscientists to...promoters and/or fluorescent tags are available at Addgene. Shemesh OA, et. al. Nature Neuroscience. 2017...The design use to detect PKA activity can be generalized to measure phosphatase activity, second messenger...

...experiment? What if after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation...preparation is quality controlled through next generation sequencing and spectrophotometry to test purity...Suggestion Form.  Additional resources on the Addgene blog Browse blog posts from our Plasmids 101 blog...the various ways to quantify DNA Resources on Addgene.org Learn about our other beta projects Read our...

...opportunity. That's how Addgene was founded. That's how my position and my team at Addgene was created.”- Eric...discussed on the Addgene blog, consider writing a guest post. Additional resources on the Addgene blog Read...transferable skills guide Resources on Addgene.org Find job listings at Addgene Get more career insight from Addgenies...  We touch upon these topics frequently in the Addgene blog. In fact, we have so many blog posts about...into one comprehensive, downloadable guide: the Addgene Science Career Guide. We also asked Addgenies what...

...the antibiotic resistance gene and origin will replicate, and the transgene will be lost. Over time, bacteria...vector backbone contains the antibiotic resistance gene (AmpR) and origins of replication (ori and f1 ori...these cloning strains, browse the table of common gene mutations in our Plasmids 101: Common Lab E. coli...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...recombination, one resulting plasmid carries the transgene of interest (aka the viral genome) and the other...10.1186/s12934-020-01321-4   Kolodner R (1980) Genetic recombination of bacterial plasmid DNA: electron...10.1261/rna.069286.118 Additional resources on the Addgene blog Find more molecular biology and viral vector...

...2018), and hybridomas can lose gene expression of the mAb-encoding genes, die, or fail to revive after ...broadly, advances in gene synthesis, affinity reagent screening, and next-generation sequencing of hybridomas...grants and Addgene’s mission This is the first NIH grant that Addgene has received. Addgene prides itself...allow Addgene to further our mission of scientific sharing,” says Dr. Daniela Bourges, Addgene’s interim...opened the door for a new generation of recombinant affinity reagents. Addgene is looking forward to working...antibodies Resources on Addgene.org Find recombinant antibody plasmids at Addgene ...to improve upon these critical research tools. Addgene is excited to announce that we have recently received...

...targeting a gene associated with diphtheria toxin lethality and were sorted into single wells to generate clonal...instance, it’s much more difficult to introduce a gene of interest into primary cells, so most primary ... risk of viral integration causing mutations and gene expression changes elsewhere in the genome. Second...j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and reporters Previous...

...simultaneous activation and/or repression of multiple genes, and more. The researchers now plan to adapt the...powerful tools to modify the expression of multiple genes at the same time.” For more details on how to apply...the Mulitplex CRISPR/Cas9 Assembly System Kit at Addgene here. Other Popular CRISPR Posts: How to Design...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... Plasmids podcast series, you'll learn about optogenetics tools for controlling protein activity, methods...Base Editors Hot Plasmid 4: pPSU Plasmids for generating DNA molecular weight markers.  Listen to the ...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ... optogenetics...

...in applying gene technology to bacteria and plants. Her research involves cloning cry genes from the soil... shared mission of both Seeding Labs and Addgene. The Addgene-Seeding Labs plasmid grant is a collaborative...of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for.... Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check...introduce the 5 scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists...plant biology, she coordinates the Network for the Genetic Improvement of Cowpea, a knowledge database for...potential agricultural and medical use. Find More Optogenetic Tools Like pDawn  Making cassava plants resistant...

...protein coding genes in E. coli and S. cerevisiae expression vectors are available at Addgene. A set of mammalian...yeast expression constructs contain protein coding genes, tags, truncations, and codon optimizations similar...of plasmids containing SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors ... available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids...been functionally tested by Ginkgo Bioworks or Addgene. If you used plasmids from this collection in your...COVID-19 Plasmid Collection Quality control at Addgene in the midst of COVID-19 We received Ginkgo’s first... our standard full plasmid sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids...

...expression of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated from mouse fibroblasts...differentiate into specialized cell types [2]. We general speak of 4 levels of stem cell potency: Totipotency... in many organs in the adult human body. These generate specialized cells for their location, mostly to...isolated from the human body Usage Universal. Can generate all adult cell types. Limited. Only specific cell... realistic options for scientists working in regenerative medicine, but the longing for a model system...Yamanaka and Thomson labs showed the successful generation of human iPSCs from dermal fibroblasts via retroviral...Prize for Dr. Yamanaka in 2012. Find plasmids for generating iPSCs iPSCs and ESCs closely resemble each other...

...for the generation of one or more mutations – it is as easy as obtaining a plasmid with the gene of interest...interest (search Addgene plasmids by gene here), designing primers with your desired mutation, performing... desired promoter, selectable marker, and target gene, for example, are amplified with overlapping regions... E.coli for plating, screening and generation of sgRNA. Generation of sgRNA libraries, with maximum complexity...multiple mutations in one or multiple fragments, and generate constructs for producing single-guide RNAs – but...the NEBuilder HiFi DNA Assembly Mix include:  Generation of constructs with multiple point mutations Cloning...sgRNAs Production of Yeast Expression Cassettes Generating constructs with multiple point mutations NEBuilder...

...inhibition library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening...types, recombinases allow you to further restrict gene expression to areas in which the recombinase and... patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and GFP....pAAV-hDlx-Flex-GFP-Fishell_6 Gordon Fishell   Optogenetics Optogenetics continues to supply neuroscience with ...mCherry-Fishell-3 Gordon Fishell   Chemogenetics Chemogenetics offers noninvasive methods for manipulating...help@addgene.org with the vector name and serotype you’d like to be notified about. Chemogenetics Plasmid...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...

... nucleic acid sequence of the antibody-encoding genes over time as the hybridoma cells divide. These changes...before culturing or they can clone the genes from the hybridoma into a plasmid to create a recombinant...Figure 2: Changes to the heavy chain or light chain genes within a hybridoma impacts the specificity, avidity...Resource on Addgene.org Check out Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody...above, genetic drift is one concern when using hybridomas to produce monoclonal antibodies. Genetic drift...the target molecule. Although it’s possible to generate antibodies against many types of molecules including...recognize proteins. Figure 1: Steps in the generation of hybridomas for monoclonal antibody production...

...her current research, Sheen aims to identify new genes related to PZA-resistance by using different approaches...as pACBSR-hyg and pFLP-hyg will be used to create gene deletions of important unknown regulatory regions...growth will then be measured to link the deleted genes to specific functions in biofilm formation. The...on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out Addgene-Seeding...of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for...Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment...resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to ...

...exhibiting its versatile utility in quantifying multiple gene editing activities. Click here to find the ACE ...Wanisch  Listen to the optogenetics podcast segment LOVTRAP is an optogenetic system that was recently... the University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 and Zdk1, where...function is more specific and much faster than any genetic or pharmacological manipulations on cells, and ... riboswitches used in the Riboglow platform at Addgene. Braselmann E, et al. Nature Chemical Biology...

... we can make Addgene better and make scientists’ lives easier. Why transition to Addgene? So why did ...more satisfying, because Addgene is a non-profit, we can focus more on generating blog content that scientists... that PhD. Resources at Addgene Read Other Careers Posts Visit the Addgene Careers Page Learn about ...Harvard University and entered a new role here at Addgene as an Outreach Scientist. I used to spend my days... less time wrangling bacteria and instead help Addgene fulfill its mission of helping scientists share...did I join Addgene? Was I jaded by my time in academia? Did I run screaming from the lab like the cartoon...didn’t, it would have been unwise of me to join Addgene. This is particularly true in my position as an...

...domain due to a gene that resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2...sequence similarity, phylogenetic analysis, neighborhood analysis of surrounding genes, and experimental ...straightforward as it seems. There is not a single gene that is shared across all CRISPR types; the fast...together because they contain a distinct Cas7-type gene. The exact mechanism of type IV systems is not known... and applications with the type I CRISPR system. Gene and Genome Editing, 3–4, 100013. https://doi.org...the array of CRISPR technologies. Learning the general principles of this classification system will help...A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A. Cas9 was the first to be adapted...