We narrowed to 645 results for: Rest

...discovered? The discovery of GeoCas9 started with an interesting question: why engineer a new Cas9 when you can...CRISPR Methods for Bacterial Genome Engineering   Interested in using CRISPR/Cas9 RNPs? Check out this post...

...added to the N or C terminus of your protein of interest. Many of these tags also have commercially available...makes it amenable to being left on your protein of interest, especially if downstream antibody detection is...

...conditions without necessarily modifying the gene of interest in any way. For example, if green fluorescent ...situations. Tissue-specific genes – the genes of interest may not be expressed in the tissue/cell line you...

...works to meet the individual needs of schools interested in using eScience Labs as part of their curriculum...Learn more about alternative science careers here Interested in creative science education tools? Check out...

...allow you to test the function of Your Gene Of Interest (YGOI) in a controlled environment under various... downstream of the open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer...

... had an alter ego as a mad scientist for show wrestling, founded a nonprofit, learned to play the ukelele...interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation builds...

...mice, by replacing the effector with a gene of interest in the cells you are studying. Read our SPARC ...single molecule-fluorescence assay to monitor restriction enzyme digestion of single molecules in real-...

... development was something Stegmeier has been interested in for a long time. “At an early age, I was fascinated...reagent, it’s an annotated reagent.” He’s also interested to see Addgene expand the sharing platform to...

...downstream applications (Berns, 1972). Using qPCR and restriction enzyme based methods, we showed that the DNA ...easily customized to detect other sequences of interest.   While the VGS process isn’t new to Addgene,...

...inducible CMV promoter and targeted to a sequence of interest with an sgRNA expressed from a separate plasmid...dCas9–APEX2 biotinylation at genomic elements by restricted spatial tagging), to confirm the composition ...

...blindness’ - the tendency for students to be more interested in studying animals than plants, and biology ...platform soon to be released. For individuals interested in ordering from ABRC for the first time, we ...

...have in common? You can do quality science on interesting topics Having strong non-science skills is important...mentors, to discuss your strengths, weaknesses, interests and passions is another great way to shed light...

...getting researchers to the experiments they’re interested in faster by producing, titering, and testing...neuronal activity while studying your phenotype of interest. Optogenetic tools for similarly controlling neuronal...

...scientists can use these to tag their proteins of interest. Antibody based probes, such as antigen-binding...epitope from the CDRs.  To visualize proteins of interest tagged with the HA epitope, the lab fused a fluorescent...

...a graduate student at Kyoto University. He is interested in developing genetic tools for neuronal circuit...professor at Kyushu University. He has a particular interest in the development of sensory systems focusing...

...display libraries In surface display, proteins of interest are fused to anchoring proteins to be expressed... few steps! To check whether a library you’re interested in is available as a viral prep, look under “...

...with the viral plasmid containing the cargo of interest, and HEKs will produce and package viral particles...be a great option! Especially if your research interests include receptor biology, ion channels, or involve...

...candidate with extra experience or a marketing interest that would be able to apply themselves immediately...immediately to the position. In case you are interested yourself, sales and marketing positions like these ...

... Mentee: Make a list of topics/skills you are interested in exploring. Mentor:  Make a list of topics/...smaller, actionable steps (e.g. Meet 10 PhDs with interesting careers, find a summer internship, etc.). It ...

...optogenetics advance, the big questions that motivate his interest in neuroengineering, and some of the optogenetic...calcium or some other specific ion that would be interesting. It would also be great if you could make neural...

...governed how this game was played. These rules restricted which cards could be played when and, depending...founder and consultant at FRESCI particularly interested in advanced in vitro models technology. Follow...

...than those without Addgene deposits? Even more interestingly, could we tell if depositing a plasmid with ...Sloan School of Management. She is particularly interested in exploring how innovative tools influence the...

...You can also enhance expression of your gene of interest with a variety of Cas9 activators available in...model that reflects the underlying biology you're interested in studying. You need to make sure your assay...

...conditionally and reversibly degrade proteins of interest fused to a degron, a domain that induces degradation...This toolkit makes it easy to tag your protein of interest to create conditional human cell lines. Lastly...

... Professor in Microbiology, and her group are interested in understanding the mechanisms behind Mycobacterium... Julio Ernesto Valdivia Silva is a master of interesting combinations: biomedicine and astrobiology. During...

...understanding how, when, and where TFs work is of broad interest to the scientific community. Recently, the Zhang... The TF map is a valuable resource for anyone interested in cell differentiation programs. The Zhang lab...

...tissue types, recombinases allow you to further restrict gene expression to areas in which the recombinase...expression patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and...

...about the various posters I visited. If you’re interested in neuroscience but are afraid you don’t have...virus for you. Neuroscientists are particularly interested in using AAVs because they provide a highly versatile...

...open-reading frame and separated from the protein of interest by a 2A self-cleaving peptide (Goedhart et al,...correlation between the fluorescent protein of interest and the reference protein (Goedhart et al, 2011...

...RNA (gRNA) used to target the genomic locus of interest. As these off-target effects can be genome-wide...can allow for targeting of your genomic loci of interest that may not be accessible by wild-type Cas9, ...

...and then unfollow those who do not capture your interest during that week.” You can also find Dr. Knoepfler...consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve: 1) convinced...

...partnership with high school teachers to boost interest and understanding around the engineering of biology...by emailing [email protected]. Of particular interest this fall:  The BioBuilderClub BioBuilderClub ...

...the intent is to make a knockout in your gene of interest, as it is prone to generating indel errors. Indel...bloggers!   David Wyatt is a graduate student interested in determining how the structure of broken ends...

... allows synthetic biologists to use Type IIS restriction sites to easily assemble multiple DNA fragments...dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no...

...along with an antibody targeting the protein of interest led to rapid, targeted degradation that is dependent...independently control expression of your genes of interest. These transcription factors consist of the GAL4...

...s start with the paper itself: Ma et al. were interested in correcting an autosomal dominant mutation ... vs repair from the maternal MYBPC3 allele. Interestingly, they found that the embryos underwent repair...

...growth factors or serum to stimulate a pathway of interest, but there can be a lot of static since other ...inserting pdDronpa domains into loops of a protein of interest. If you plan to try this, it’s important to check...

...assay with your chosen FP in your cell type of interest before starting your experiments.  Selecting a...this fluorescent protein to tag your protein of interest will be problem-free. In figure 1 we provide an...

... approach to simultaneously clone two genes-of-interest (GOI) into two independent expression cassettes...pUC57-L3L2) that offer the advantage of using restriction digest cloning to introduce the GOIs into the...

...considered "natural tumor suppressors." A growing interest in the cancer microenvironment Ever since Stephen...Viva" in Aveiro, Portugal. She is particularly interested in science journalism and writing about biomedical...

...every project, time, attention, and/or length restraints mean you will have to pick and choose what concepts...feedback in a variety of ways, like having them restate or summarize the concepts you’re covering. Don'...

...When we developed this tool, there were so many interesting questions that we could apply [it to].” Using...Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned about tumor ...

...scientists can control expression of their genes of interest. While recombinases are powerful gene expression...cells to identify cells exhibiting properties of interest. However, FACS is unable to spatially and temporally...

...on a guide RNA complementary to the sequence of interest. Upon binding, CRISPRa systems recruit transcription...having multiple copies of VP64 at each loci of interest, this allows more transcriptional machinery to...

...take me. I’m also just learning. I have a huge interest in data science,” which she’s taking some classes... hours in the lab, she told me, “I was really interested to learn more when I was introduced to bioinformatics...

...the G receptor. In neurons, the G receptor is restricted to presynaptic nerve terminals (Lafon 2005). ...applications Use of G deleted RABV in conjunction with restricted TVA and G protein expression is a powerful development...

...plasmids are also used to teach students about restriction mapping and western blotting. Students who participate...genetics at Boston College. She is particularly interested in strategies to incorporate research into undergraduate...

... CRY2 and CIB1 domains to separate proteins of interest, a researcher can cause them to interact by stimulation...reversible oligomerization when fused to a protein of interest. Fusion protein oligomerization happens within...

...SpCas9. gRNA A gRNA targeting a DNA sequence of interest is expressed in 293T cells and loaded into Nanoblades...transduction for most cell types they tested. Interested in using Nanoblades? Plasmids are available from...

...particles are delivered into the brain region of interest. AAV or lentivirus?  Both AAV and lentiviral vectors...however, the spreading of vector to the area of interest may be very limited with this method, especially...