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Showing: 141 - 160 of 360 results

  2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...that can be imaged with the optics on the microscope(s) you intend to use. An accurate determination of whether...protein that is supposed to be extremely bright. In S. cerevisiae, we've tested a number of green and red...

  3. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ... incubation temperature for your specific protein(s) of interest.  Determining linear detection range ...assay or BCA assay. Be sure to aliquot your sample(s) before adding in your denaturing and/or loading buffers...that covers a size range appropriate for the target(s) and transfer method. Membranes The two membrane ...your standard curve, your standard, and your sample(s), as you can only compare samples to a standard curve...using a protein stain like Coomassie Blue or Ponceau S. It is far less prone to error than normalizing to...

  4. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...blog PRIME Editing CRISPR 101: Cas9 vs the Other Cas(s) Progress Towards a PAM-free CRISPR...

  5. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An optimized fluorescent probe for visualizing...PubMed Central PMCID: PMC4469972 Marvin, Jonathan S., et al. “Stability, affinity and chromatic variants...

  6. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone... XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are not monospecific...

  7. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun ...Agbandje-McKenna M, Cleaver BD, Clément N, Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant...

  11. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity can be thwarted if no dNTPs are...doi: 10.1007/978-1-61779-564-0_5. PubMed. JBEI-J5’s guide to SLIC Resources at Addgene Ligation-Independent...

  12. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...https://doi.org/10.1101/2021.09.07.459259 Sarabipour S, Debat HJ, Emmott E, Burgess SJ, Schwessinger B, Hensel...10.1371/journal.pbio.3000151 Sever R, Roeder T, Hindle S, Sussman L, Black K-J, Argentine J, Manos W, Inglis...

  13. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...controls as you get your assays up and running.  Ed.'s note: More questions on antibody validation? Check...availability References Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez...

  14. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...making!  References and Resources Kulanayake, S., Tikoo, S. K. Adenovirus Core Proteins: Structure and ...

  15. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ... https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I., Iranzo, J., et al. (2019). Evolutionary...CRISPR experiment? CRISPR 101: Cas9 The Other Cas(s) The PAM Requirement and Expanding CRISPR Beyond ...

  16. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ... Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been widely popular in...Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes CRISPR chimeric RNA element with customizable...

  17. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5547-51. doi: 10.1073/pnas.89.12.5547...: 27730575; PMCID: PMC5270762. Wendler, F., Park, S., Hill, C. et al. A LexAop > UAS > QUAS trimeric plasmid... the trp and lac promoters.” Proc Natl Acad Sci U S A. 80(1) (1983): 21-5. PubMed PMID: 6337371. PubMed...

  18. Hot Plasmids: Fall 2025

    Type
    Blog Post
    Published
    Nov. 11, 2025, 2:14 p.m.
    ...doi.org/10.1101/2024.12.16.628673 Zhou, S., Zhu, Q., Eom, M., Fang, S., et al. (2025). A sensitive soma-localized...PinkyCaMP plasmids and FRCaMPi plasmids! Fink, R., Imai, S., et al. (2024). PinkyCaMP a mScarlet-based calcium...

  19. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...Yamamoto, H., Hirasawa, A., Ennishi, D., Tomida, S., Toyooka, S., & Otsuka, M. (2025). Genomic Differences ...Peach, C. J., Kilpatrick, L. E., Woolard, J., & Hill, S. J. (2021). Use of NanoBiT and NanoBRET to monitor...

  20. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal genome...Ousterout DG, Thakore PI, Moreb EA, Rivera RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D,.... pii: aad5143. PubMed PMID: 26721684. 4. Reardon S. Gene-editing summit supports some research in human...
Showing: 141 - 160 of 360 results