...culture through various sources that are difficult to trace. These include the laboratory personnel, the serum...the flasks (unless the source of culture is irreplaceable or highly expensive). Detecting mycoplasma contamination...mycoplasmas, contamination by other bacteria or extracellular fluorescent signals produced by these bacteria...culture flasks. In cases where the culture is irreplaceable or very expensive, there are some ways to rescue...cultures because you are not the first person to face this kind of problem. Educate others when you can...

...important of those is to always look closely at the trace file (or chromatogram) of the sequencing results...sequence (no Ns in sight). If you never looked at the trace you would be happy. But look closer, the overlapping... 2. You should see individual, sharp and evenly spaced peaks …like these…. 3. Expect to get 500-700 bases...you can purify the product either via the Sodium Acetate/isopropanol precipitation method or using a silica...

...or by project, if that works better for you) and place them on an easy to reach shelf. Make sure all notebooks... Post-its and write brief descriptions on each. Place the notes sticking up out of the top of the notebooks... notebooks (ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to...find to where the binder was moved. You’ll have peace of mind knowing the information is backed up and...

...to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever more... structure to interrogate the sequence isn’t in place yet.     Figure 4: S. pyogenes Cas9 in the...perfect guide RNA will do you no good if there is no adjacent PAM to anchor it. However, Cas9 only checks the...HNH domain, ensuring all binding partners are in place before cleavage can occur (Jiang & Doudna, 2017;...

...talk about Addgene’s internship opportunity taking place next summer.  I applied to Addgene and began the...first week at Addgene, I had to get used to the workspaces, as well as complete the lab safety and cybersecurity... clones because the bands were all in the wrong place. Except for one. My manager speculated that the ...safety, general work skills — such as using Google workspace — and got the chance to work on my very own research...

...best place for the biosensor to be expressed. Are you looking at an intracellular or extracellular molecule...calcium sensor CMV-mito-GEM-GECO1  and blue flame extracellular glutamate sensor pCMV(MinDis).iGluSnFR. Picking...

...special words about managing scientists because, let’s face it, we are special. Below are some special characteristics...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...approached as peers rather than subordinates Need mental space and dislike intrusions (this actually varies, a ...scientists) Are highly mobile and can move to a new workplace if opportunities for learning and personal growth...

...science writing in magazines or newspapers or other places online, and consider how those stories are being...their blogs. 5)   Local science museums are a great place to find opportunities for science outreach. Here...institution; the news or communications office is a good place to start. It’s not unusual to find science writers...Mass Media Fellowship or maybe an internship at a place like Science News. Ask your institutional news office...

...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex...blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes...genes. These data highlight an interesting "arms race" between phages and prokaryotes; the ultimate victor...

...some marketing experience, with little emphasis placed on the marketing or business background of the ...focus on the list of conferences I presented at and placed them under headlines entitled “Presentation Experience...more than necessary for my interview. The simplest place I could think of to start was “Marketing for Dummies...outside of academia, sales and marketing can be great places to start. Depending on what type of career you ...

...originally published in Nature, is a “search-and-replace” genome editing technique that mediates targeted... efficient due to its lower dependence on PAM placement. Fewer byproducts and more efficient than homology-directed... formation slightly. Designing the sgRNA with a spacer that only binds the edited strand, as in the PE3b... & Liu, D. R. (2022). Programmable deletion, replacement, integration and inversion of large DNA sequences..., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand breaks or ...

...remember that industry career paths are not the only place to develop “industry related skills”. Many career...self-driving cars, tissue engineering for organ replacement).  In addition to presenting your expert skills...Google Scholar account Sanitize your LinkedIn, Facebook profile, online presence (if you need to).  Immigration...for international candidates. Being able to “put a face to a name” will help a candidate get looked at and...investment banking and venture capital areas.  We place individuals into roles in areas including: research...

...and novelty in scientific research and in any workspace. Re-design lab meetings with a focus on discussion...making lab meetings more interactive and creating space for discussion at the end of each talk - time for...remotely in science are endless. Let’s adapt and embrace new ways of working, reconnect as human beings ... purposeful and creative collaboration in the workplace. There are many other ways to encourage collaborations...

...both conferences, there was a Q&A section in the interface where you can type in questions for the speaker...posters could be viewed publicly and stored in one place if the participants used the conference hashtag ... says.Depending on the meeting, there may be a “place” for networking within the app. For example, the... an on-site conference, you really have to make space for it,” says Bachle. “On the flip side, you can...

...Biology and, on occasion, would meet in the same space where BosLab exists today. Back then, it consisted...Kendall square, but we saw the potential in the space and decided to reactivate it. We started out by ...projects at BosLab. Some are entrepreneurs using our space to perform early-stage experiments. For example,... visit our website and follow us on twitter or facebook. If you are local, join us for our next event ...

...example the Phage Assisted Continuous Evolution (PACE) method requires constant turbidity of the culture...its native endonuclease activity degrades the displaced strand.   Figure 1: EvolvR consists of ... of PolI3m. This tag would allow researchers to trace the progeny of a single starting cell. Additionally...generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources ...

... by spaces. File names with more than one word should be named in the camelCaseFormat since spaces between...analyze large data sets (myself included) and we are faced with finding the best system to document these types...the basics. However, these notes take up a lot of space and don’t tell you why you did something or what...

...amazing affinity reagents we just didn't have the space to give proper credit to. Thankfully, as Addgene's..., or be able to access an epitope folded into a space that would size-exclude an antibody. In imaging ...eukaryotic cells, allowing for them to be used as intracellular inhibitors.  Aptamers are not proteins at all.../neu and CD16 Exhibit Improved Tumor Lysis When Placed in a Divalent Tumor Antigen Binding Format. Journal...

...Personalize all connection requests LinkedIn is a place to build relationships. If you don’t add a personal... talked about (or how good the beer was at that place, for example). This is also a great way to keep ...profile and not updating it. LinkedIn may actually replace the resume some day (I’ve already abandoned my ...have to be a headshot in a suit, but this is not Facebook so no pets or kids. Try looking a bit over your...

.... Everything will go wrong Whenever an order is placed on the Addgene website, it sets off a chain reaction... need to be repeated. We also now have a good interface to begin tracking analytics data for lab procedures...at some point, decide early on what to do in a namespace collision. It may even be worth it to have extra... you're solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence...