...priorities in most cases include IP protection, publications and funding. If any of these three pillars crashes...not enough. A 2013 study showed that out of 238 published scientific articles, only 46% could be reproduced...there will be no sharp changes in costs once they establish the system within their institution. In the study...

...data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist can learn....room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs have indirectly... done so. How can you expect to deliver a great public presentation if you are doing it for the first ...

...synthetic biology, mainly because of the ease in assembling multiple fragments in one step with no scar sequences...CRISPR technology and is described in a recent publication by the Lockey lab (Wang, et al. 2015). In this...PMC4569707. 4. Kahl, L. J. & Endy, D. A survey of enabling technologies in synthetic biology. J. Biol. Eng...

...the right information When using a previously published system in your own work, you’ll need to know (...break your experiments!  First, closely read the publication, including the supplemental data. You’re most... both of which have extensive collections of publications and information on hardware, optogenetics tools...

...need to use FACS (like they did in the original publication). I thought I could see GFP expression with a...expression, but I didn’t. 2. In the original publication they see GFP expression in about 20% of their...an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use multiple gRNAs to target different...

...speed at which a confocal image can be acquired. Assembling an image pixel by pixel is slow; therefore, certain...can a a high enough density of red-photons be establish for this to happen.Using red light has a second... be afraid to seek out expertise from a well-established microscopist at your institution when heading...

... intermediate pluripotent state is not always obligatory in cell conversion processes [35]. Rather than...Complete removal to convert to iPSCs and re-establishment after Partial rearrangement from one cell type... pursue a career in science communication and publishing after completing her postdoctoral training from...

...The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin... abundant targets: from variable nonsense to publication quality data. Sci Rep 7, 2409 (2017). https:/..., J. The Ultimate qPCR Experiment: Producing Publication Quality, Reproducible Data the First Time, Trends...

...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...the above CRISPRi section, the Marraffini Lab published a robust tool for CRISPRi and CRISPRa using RNAP-Omega-dCas9...RJ, Grossman AD, Gross CA, Rosenberg OS (2019) Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...

... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...PubMed PMID: 26258682. Find plasmids from this publication at Addgene. Resources on the Addgene Blog Read...

...you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed...to set-up emails to alert you when papers are published on your favorite topic. If you have any other...

...A newly established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering.... “However, there had been no systematically established way of making an all-in-one vector for multiplex...

...the experiment. If that data was included in a publication, you'll find that information in the report as...completeness. The information is then formatted and published as a report with a DOI for citation. If you’re...

...'s Addgene ID # has been referenced in other publications, you'll find a link to the list of citing articles...cell-surface programmable DNA-binding domains" publication.  If you want to see this new feature in action...

... This blog was originally published on BitesizeBio here. As part of my job ensuring plasmid quality at...Acknowledgements: Thank you BitesizeBio for originally publishing this and allowing us to share it with our readers...

...one “safe” project that you know you can get a publication with and one “shoot for the moon” project that...Joseph Campbell, and read about his "follow your bliss' mantra and never forgot it. Usually when I see ...

...cells but extends to freely moving animals, as established for conventional optogenetic tools." In total...applied the LITE system to 28 different genes to establish it as “a novel mode of optogenetic control of ...

...the goal of communicating science to the general public and training the next generation of science communicators...source of scientific information for the general public as well as a platform for scientists to be viewed...

...listed in the table has been used effectively in a published article. This blog post will serve as a guide ...and final experimental outcome in the original publication. As you develop and confirm new gRNAs, please...

...restriction enzymes for cloning (see schematic below), enabling a researcher to test multiple tags for one protein...introduction to pCri, please see the original publication cited below. pCri plasmids are available either...