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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 458 results for: BLI

Showing: 141 - 160 of 458 results

  1. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...protein instead localizes to the cytoplasm? Or more troubling, what if a significant fraction of the fusion ...properly? We suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5... non-optimized parental proteins. Our recent publication illustrated the quantitative increase in fluorescent...

  2. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...priorities in most cases include IP protection, publications and funding. If any of these three pillars crashes...not enough. A 2013 study showed that out of 238 published scientific articles, only 46% could be reproduced...there will be no sharp changes in costs once they establish the system within their institution. In the study...

  3. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist can learn....room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs have indirectly... done so. How can you expect to deliver a great public presentation if you are doing it for the first ...

  4. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...synthetic biology, mainly because of the ease in assembling multiple fragments in one step with no scar sequences...CRISPR technology and is described in a recent publication by the Lockey lab (Wang, et al. 2015). In this...PMC4569707. 4. Kahl, L. J. & Endy, D. A survey of enabling technologies in synthetic biology. J. Biol. Eng...

  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...the right information When using a previously published system in your own work, you’ll need to know (...break your experiments!  First, closely read the publication, including the supplemental data. You’re most... both of which have extensive collections of publications and information on hardware, optogenetics tools...

  6. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...need to use FACS (like they did in the original publication). I thought I could see GFP expression with a...expression, but I didn’t. 2. In the original publication they see GFP expression in about 20% of their...an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use multiple gRNAs to target different...

  7. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...speed at which a confocal image can be acquired. Assembling an image pixel by pixel is slow; therefore, certain...can a a high enough density of red-photons be establish for this to happen.Using red light has a second... be afraid to seek out expertise from a well-established microscopist at your institution when heading...

  9. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin... abundant targets: from variable nonsense to publication quality data. Sci Rep 7, 2409 (2017). https:/..., J. The Ultimate qPCR Experiment: Producing Publication Quality, Reproducible Data the First Time, Trends...

  10. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...the above CRISPRi section, the Marraffini Lab published a robust tool for CRISPRi and CRISPRa using RNAP-Omega-dCas9...RJ, Grossman AD, Gross CA, Rosenberg OS (2019) Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...

  12. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed...to set-up emails to alert you when papers are published on your favorite topic. If you have any other...

  14. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...the experiment. If that data was included in a publication, you'll find that information in the report as...completeness. The information is then formatted and published as a report with a DOI for citation. If you’re...

  15. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ...'s Addgene ID # has been referenced in other publications, you'll find a link to the list of citing articles...cell-surface programmable DNA-binding domains" publication.  If you want to see this new feature in action...

  16. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ... This blog was originally published on BitesizeBio here. As part of my job ensuring plasmid quality at...Acknowledgements: Thank you BitesizeBio for originally publishing this and allowing us to share it with our readers...

  18. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...cells but extends to freely moving animals, as established for conventional optogenetic tools." In total...applied the LITE system to 28 different genes to establish it as “a novel mode of optogenetic control of ...

  19. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...the goal of communicating science to the general public and training the next generation of science communicators...source of scientific information for the general public as well as a platform for scientists to be viewed...

  20. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...listed in the table has been used effectively in a published article. This blog post will serve as a guide ...and final experimental outcome in the original publication. As you develop and confirm new gRNAs, please...
Showing: 141 - 160 of 458 results