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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 659 results for: Clu

Showing: 141 - 160 of 659 results

  1. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...the template. The PE3 system does just this by including an additional sgRNA. Using this sgRNA, the prime... number of unintended prime editing outcomes, including indels. Therefore, they engineered a dominant-...     ✓ ✓ reflects an innovation always included in the indicated tool. ~ reflects an innovation... strategies in our CRISPR guide. Some examples include: TwinPE: two prime editors make complementary ...

  2. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...species, antibody species, or whether the results include knockout data.    Figure 2: Search options in ...description of the experiment. If that data was included in a publication, you'll find that information... is easy!  Here at Addgene, we love all data, including negative data, which can help researchers save...

  3. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    Published
    Feb. 25, 2021, 2:15 p.m.
    ... to a post-academic career. This training was “included” with their tuition, so to speak.  Unfortunately...the development of priorities for pd|hub which include building capacity to identify the most effective...scientists, but it is essential that we integrate inclusive career readiness training in this educational ...

  4. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...per gene and includes ~10,000 gRNAs targeting intergenic regions. The libraries also include 2,400 gRNAs...recently deposited to Addgene and you’d like it to be included in the next What’s New in CRISPR blog post, please...

  5. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ... show resistance to stressful circumstances – including unpredictable loud noises and flashing lights ... of the animals’ skeletal muscles led to another clue: differences in the kynurenine pathway of tryptophan... treatments for a variety of brain disorders, including psychiatric and cognitive conditions as well as...

  6. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...White House honored 13 “Champions of Change,” including our recent guest Drew Endy, in celebration of ...trouble, even when all kinds of other factors, including journal impact factor, study topic, and the corresponding...review. 8)     That same issue of Science also included an online survey of scientists showing overwhelming...

  7. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...attracted much attention over the past few years, including an article in the BBC Wildlife Magazine. It is...people for years and have gathered much acclaim - including being featured in the National Geographic and ... are now taking place and gathering momentum, including in Cambridge and Bristol. If you’re a scientist...

  8. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...team reveal the mechanism behind its activity including an extensive RNA scaffold and a domain needed ...inhibition library from the Chong Zhong lab. Now, we’ve included another E. coli genome-wide inhibition library...recently deposited to Addgene and you’d like it to be included in the next What’s New in CRISPR blog post, please...

  9. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...don't read, you're doomed.” 2. Always be sure to include various positive and negative controls to “convince...entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped away from...Blog Series   Our 3 PIs answer more questions, including: Advice for Choosing a Research Project Finding...

  10. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...submissions via email: [email protected] Entries include accession number, species, and assigned category...profiling for human, mouse, and dog cell lines is included when available, and can be submitted via email...Depending on available information, entries may also include whether a cell line is known to be genetically ...

  11. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...straightforward in cases where the inserted DNA includes an antibiotic resistance marker. After transformation...grow. However, often times, the plasmid backbone includes an antibiotic resistance cassette and you aren...Gateway cloning takes advantage of this property by including ccdB on its destination plasmids. In the destination...

  12. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...years of study to come. Some Freshers Fairs can include fairground rides and other fun activities and even...society organises talks by prominent scientists, including Nobel Laureates, who speak about their work and...recreational and educational events throughout the year, including a Christmas Chemistry symposium and a career night...

  13. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...thickness. The fibers are drawn with microelectronics included in them for downstream control – this is important...components are added - the ‘bells and whistles’ – including microscale light emitting diodes, thermal sensors...neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar neural fiber...

  14. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...allow researchers to understand bacterial gene clusters that produce useful natural materials, such as...are some highlights from the past couple months including several pooled libraries: Cas13X and Cas13Y, ...sensors from Yulong Li’s lab are now available including: pAAV-hsyn-GRAB_DA2h in AAV9 New GRAB-DA and ...

  16. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...the mid-20th century, and many iconic papers, including the ones on the structure of the DNA, were not...first couple of sentences - even if written in a clumsy language make it much easier for me to go back ...initial read, adjust my language in the first (and clunky) version of my general comments and adjust them...

  17. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...protease. The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for affinity purification...purification (see plasmid map and cartoon below). Including biotin in your culture and inducing protein expression...binding kinetics using a multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences...

  18. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ... by any number of characteristics. Common ones include age range, familiarity with the subject, shared... that by defining a specific audience, they’ll exclude others from accessing their work. But that’s not...science communication work does not explicitly exclude people outside of the original recipients. Instead...

  19. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...RNA-seq clustering of cells transduced with the TF pooled library. Louvain community clusters of individual...Collection) and an accompanying expression atlas, including over 3,500 human TF isoforms, to help researchers...

  20. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...protein, etc...).  There are many online guides – including an excellent series of posts by Joachim Goedhart...risk of inaccurate data submissions, so FPbase includes a versioning system that allows users to see when...improvement and elaboration.  Current projects include incorporation of biosensors, protein evolution ...
Showing: 141 - 160 of 659 results