We narrowed to 799 results for: Comp;

...lab. Iron Chef-style competitions Every few months, we’ll have a cooking competition featuring a selected... eat together every day, sharing ideas for the company, but also talking about sports, movies, and just..., which is either an indication of the stiff competition or says something about my cooking.   This is... to organize. We’ll dedicate a post to these competitions in the future, and hopefully include some of...To-Do list long enough that there is no hope of accomplishing it all. How do we encourage people to take a... Hack Days, and we’ve seen great success at accomplishing “we-should-really-make-time-for-that” type projects...

...based on its library frequency before selection compared to its library frequency after selection. Key ...and easy technique that can be used to generate complex libraries of mutant plasmids ready for deep mutational...libraries, they all have inherent shortcomings compared to one pot saturation mutagenesis. Error-prone...vector with Gibson assembly. While this is a comprehensive mutagenesis method, a drawback to using Gibson...scanning libraries. PFunkel: Of prior existing comprehensive mutagenesis methods, PFunkel offers the best...second universal primer is used to synthesize a complementary mutant strand (step 2 and 3). Then the uracil-labeled...PCR-based approach for generating a customizable comprehensive mutagenesis library that’s ready to be tested...

...likely not a coincidence that the AAV genome is composed of single stranded DNA, which is particularly ... interest, ordering them from a gene synthesis company such as IDT or Genscript, and cloning them sequentially...infection with adeno-cre. AAV-based gene editing compared to CRISPR editing CRISPR, of course, has started...and virtually never exceeds 40%. While this is comparable or better than the efficiency of most current...systems. Ohmori et al 2017 use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template...researchers have devised methods to deliver CRISPR components as ribonucleoproteins (RNPs) that can be supplied...2017 recently showed that by delivering CRISPR components as RNPs and delivering two halves of a repair...

...now available. However, it can be difficult to compare their performance, as most translation initiation...directly engages the ribosome for translational complex assembly. PhD student Dejan Gagoski used SITS ...SITS to create the pCell-Free vectors, Gateway-compatible backbones that enable cell-free expression of proteins...-tagged human ORF clones to allow testing and comparison of different cell-free expression systems; the... construct barcoded, locus-specific libraries compatible with Illumina next generation sequencing in order...libraries using a 2-step PCR-based protocol and are compatible with single-end or paired-end read flow cells...21408-21413. PUF Assembly Kit PUF proteins are composed of repeat segments that can be engineered to bind...

... Now that you’ve completed a resource budget, and know how to decide which components are the most critical...an account of the material resources needed to accomplish your science, and can include: equipment, reagents...then start identifying the resources needed to accomplish that goal. 2. Identify the tools in that timeline...research success. If you can get away with using computer modeling and collaborations rather than using ... most start-up P.I.’s. It doesn’t have to be complicated, or difficult to create this list. Start BEFORE...equipment. That’s why a resource budget can help you compare the monetary and empirical value that helps you...from academia altogether and instead started a company around his own research. He was clear on his science...

...binary systems. In recent years, a number of more complex systems have also been developed which don’t fit... Bujard, both the promoter and transactivator components of the system have been engineered and improved...revolutionized genetic studies in flies. An additional component, the GAL80 repressor, adds a repressible element...UAS-driven gene expression. To further increase the complexity of the system, researchers have used split GAL4... developed by Lai and Lee. LexA/lexAop is a complementary system to GAL4/UAS that functions in essentially... cells and ~24,000-fold in human HeLa cells as compared to basal expression. Co-expression of repressor...because it allows them to be used together for complex genetic analysis in Drosophila or for precise control...

...Burry 2011). Complex experiments Finally, additional controls may be required for more complex experiments...expected response and have a baseline with which to compare the experimental group.     Figure 1: A...western blot several times with the same samples and compare the results across runs. If the results vary considerably...will briefly touch on.  Western blotting When comparing samples in different lanes in a western blot, ... to the controls listed above, viability and compensation controls are recommended for flow cytometry....example, propidium iodide only stains dead cells). Compensation controls address the spectral overlap that occurs... occurs during multiplexing. Compensation controls are single stained samples for each fluorophore and...

...Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst...Serotypes Serotype refers to the different protein compositions of AAV capsids, which determine how a virus ...what works great in one system or cell type can completely fail in a different system (Issa et al., 2023...designing and testing your AAV.  Figure 2: Comparison of AAV serotypes 1, 2, 5, 6, 8, and 9 in two ...Taschenberger et al., 2017).     Figure 3: Comparison of two astrocyte selective promoters demonstrating...Konishi, Y., Hosokawa, Y., & Konishi, H. (2014). A comparative analysis of constitutive promoters located in...vector-mediated transduction of the corticospinal tract: Comparison of four promoters. Gene Therapy, 28(1–2), 56–...

...reduced transformation and transfection efficiency compared to the monomeric version of the plasmid (Maucksch...multimers have additional origins of replication compared to monomers, they are also able to replicate at...bacterial cells with multimers grow more slowly compared to those containing the monomeric version of the...and more prone to being lost in bacterial cells compared to monomers, although this can be prevented via...cannot be used to detect plasmid multimers. Some companies provide NGS whole plasmid sequencing services ...frequency of multimerization, and in some cases completely prevent the formation of plasmid multimers (Bedbrook...produced the highest yield of monomeric plasmid compared to DH5α. However, more research is needed to determine...

...microenvironment, immobilizing the components and altering the composition and dynamics. The researchers demonstrated...are available, but red GECIs have been limited compared to other colors. Two recent developments add to...proteins, PinkyCaMP is the first based on mScarlet. Compared to RCaMP3 and jRGECO1a, PinkyCaMP had better brightness...to condensates, disrupting their dynamics and composition. Reproduced from Zhang et al. (2025) under CC... 2025). The team used AAV9 to deliver CRISPRa components to adipose cells to activate genes involved in... students to explore cell metabolism. Plasmid components of CRISPRkit are now available through Addgene... the Qi lab's website crisprkit.org to request complete kits or find protocols and other resources. Find...

...to detect a single, specific protein within the complex mix derived from cells or tissues. Westerns are...sample per lane, onto the top of a resolving gel composed of the crosslinked polymer acrylamide. In addition...proteins are immobilized onto a membrane, usually composed of a nitrocellulose or polyvinylidene difluoride...membrane.  In a wet transfer system the sandwich is completely submerged in a tank filled with a transfer buffer...confirming that the protein is the expected size as compared to the protein standards.  Protein abundance In...loading control when assessing protein abundance or comparing samples across lanes.  For a typical loading control...present in all samples. Uneven sample loading or incomplete protein transfer causes inconsistent levels of...

...some clones being outcompeted by others in liquid culture, dilute and plate the complete library of transformants...faces as it approaches a host cell surface are complex polysaccharide structures, many of which are easily... DNA from host cell nucleases (4). To further complicate matters, even when sequenced, phage genomes tend... for cell-binding abilities (6). This can be accomplished using a restriction enzyme with a short recognition...involves forcing DNA through a small opening using compressed nitrogen gas; this shears DNA at random locations...your resultant plasmid libraries into chemically competent E. coli and analyze transformants for appropriately...transformation should be performed using a highly competent strain that you can use for long-term library ...

...technology, and good computer skills will give you an advantage. Strong writing, computer skills (Word, Excel...you can fully investigate and solve a problem or complete a project, overcoming inevitable failures and ... Nature Photographer/Artist Ecologist     Computer and writing skills Writing a thesis or dissertation...which are useful to many employers. Increasingly, computer skills are highly desired, as roles are becoming...Will you be coordinating projects? Do you need computer programming skills? Other things to consider...as forensics currently have too many applicants compared to the number of available roles). Make your resume...

... interested in are compatible with your microscope optics, you will want to compare the excitation and...fluorescent proteins currently available. Choosing compatible fluorescent proteins To choose a set of fluorescent...scope of this post. Are your fluorescent proteins compatible with your microscope optics? To determine if ...is efficiently collected by the microscope. To compare the match between a fluorescent protein and a filter...here's a screenshot from the Chroma Spectra Viewer comparing a standard Cy3 or Rhodamine filter set (Chroma...channels used for more abundant proteins or marking compartments. I hope this sheds some light on multicolor ...

...Jurkat T cells Complexes with DNA; causes strand scissions 100-400 ug/mL *Not comprehensive.   ** In eukaryotes...cells. Of course, these mammalian vectors must be compatible with the cell type they are tranfected into –...cells. Before getting into the mammalian plasmid components, it may be useful to describe the means of introducing...called transfection. Transfection is somewhat comparable to bacterial transformation (the introduction...void. These ORIs, however, require additional components expressed in trans within the cell for effective...ORIs, respectively. The presence of these viral components greatly reduces the rate of plasmid dilution ...

...loss-of-function or neutral, as compared to wild type, would be extremely helpful as a complementary approach. Also, ...characterized? Some have developed exciting computational algorithms, such as SIFT or PolyPhen to approach... Genetic Pathway Reference Set. These Gateway-compatible entry clones are the product of many years of...cancer phenotypes. These assays were designed to complement the one assay per gene approach that has been...genes. These should be used to create ever more complete “look-up-tables” of mutant function that could...approach: Phenotypic Characterization of a Comprehensive Set of MAPK1/ERK2 Missense Mutants. Brenan L...

...clones for complete functional knockout of your target gene. Mismatch nuclease assays are completely “blind.... The drop-off probe is designed to perfectly complement the wild type sequence and is not capable of ...precision genome editing workflow. Digital PCR comparison to other validation methods As mentioned, these...96-well plate without prior quantification. Comparatively, mismatch assays require between 200 and 500... in a high background of wild type targets. Comparatively, detection limits of about 5% mutant targets...PMC3773023. 7. Vouillot, L., Thélie, A. & Pollet, N. Comparison of T7E1 and surveyor mismatch cleavage assays...

...change. Because of this, ABEs are only weakly compatible with many Cas domains beyond SpCas9. In order...lab set out to evolve ABEs with an increased compatibility to Cas homologs. Evolving better base editors...and editing speed and consists of the following components: A plasmid in the host bacterium expressing ...phage mutation rate Phage infection results in a complete ABE protein. If the phage delivers an active variant...variants with much higher base editing activity compared to the original TadA-7.10 enzyme. After testing...which edits 590-fold faster than TadA-7.10. It is compatible with all Cas9 and Cas12 homologs that the researchers...adenine base editor with improved Cas domain compatibility and activity. Nature Biotechnology. https://...

...this complex holds a pair of DNA ends together, forming a paired end complex The paired end complex then...then ligates compatible DNA ends together, thus repairing the break. This is a simplified, streamlined...processed. Processing occurs prior to ligation as incompatible DNA ends interfere with that step. Accordingly...pop-in” insertion) provided a template containing compatible overhangs is available. Cas9 can also be altered...Biochemistry and Biophysics and the Lineberger Comprehensive Cancer Center at UNC Chapel Hill.   References...

... Mediator or SAM. SAM is an engineered protein complex for the transcriptional activation of endogenous...endogenous genes and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA... assemble a synthetic transcription activation complex consisting of multiple distinct effector domains...with a BRAF inhibitor. (The test was the perfect complement for one conducted earlier by the Zhang lab for...gain-of-function screening when combined with a compact pooled sgRNA library. Our SAM-mediated screens ...sam.genome-engineering.org/. Crystal structure of Cas9 in complex with guide RNA and target DNA. Nishimasu H, Ran...transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, ...