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Showing: 141 - 160 of 356 results

  1. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...occurred once a week for several months at a time during the spring and fall semesters. Our scheduling was...Addgene bioinformatics journal club! What happens during journal club? There are many common ways to run...

  2. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...steps and outlined the considerations to be made during each step. Now that you know the basic principles...harsh - lipids and soluble proteins can be lost during fixation with these chemicals, which impacts sample...

  3. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ... of the sample name. If there was a minor error during processing, the window shows a yellow checked circle...Value - When the ICE linear regression is computed during generation of the ICE Score, the Pearson correlation...

  5. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...Planning Resources. The key to ensuring success during graduate school and beyond is to live in your lab...on what I have found to be especially beneficial during my own graduate career – and in fact, some of the...

  8. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...were kept at the appropriate temperature for the duration of their journey. Figure 2: Annual distribution...in their experiments, to enable virtual learning during the pandemic. Feedback like that lets us know that...

  9. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...terminal repeats (LTR). The 3’ LTR is duplicated during lentiviral integration, producing a second copy...voltage fluctuations, while having high fidelity during action potentials of fast-spiking neurons. The ...

  10. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...target cells Labs tend to follow the same general procedure for lentiviral infections, produce the virus, ...receptors, other important binding events take place during the course of infection. These binding events can...

  11. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...constraints given. And there were many constraints during the COVID-19 pandemic. The Pandemic Pivot Back...projects lined up, some that started production during the middle of lockdown, and some that will spin...

  12. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...stabilizes the extended open integrin conformation. During embryonic development, extended-open integrins ...development, and the shaping of the extracellular matrix. During immune responses, this same conformation encourages...

  13. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...in-vitro assays: Koch, Daniel, et al. "A pull-down procedure for the identification of unknown GEFs for small... can lead to surprisingly large fitness effects during adaptive evolution of antibiotic resistance." Proceedings...

  14. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...elongation factor that accepts Sep-charged tRNAs during translation. Many ‘flavors’ of Sep-OTS plasmids...pSer-containing GFP that can be used as a positive control during optimization experiments. This version of GFP will...

  16. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...standard protocol, but the primary antibody is omitted during the incubation step. Samples are incubated with...controls address the spectral overlap that occurs during multiplexing. Compensation controls are single ...

  17. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ... intimidating use of discipline or competence procedures Threats to personal standing – including undermining...You are not allowed to speak to lab members alone during the interview period. People seem to be hiding ...

  18. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    .... Leaving the membrane overnight in the freezer during one of these steps is also a convenient stopping...Szymanski lab (though she’s tagged along with the lab during its recent move to the University of Georgia). ...

  19. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...to transfect, select, and isolate clones. This procedure is cumbersome and inefficient, as it relies on...selection cassette can be incorporated, and used during the differentiation process. Moreover, a splinkerette-PCR...
Showing: 141 - 160 of 356 results