We narrowed to 185 results for: GRN

... day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing efficiency. On the third...

...software that predicts efficiencies of prime editing gRNAs.   PRIDICT Harvinder Virk and Michael Biddle, ...

...types. A key feature of this system is a lentiviral gRNA expression vector (pRDA_526) that (1) enables efficient...

...Most Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench (Guest...

...the transitions at target sequences specified by gRNAs. Caveats, potential specificity issues, and other...

..., programmable DNA cutting enzyme. Second, since gRNA and crRNA could be combined into one RNA to direct...

...the case of retroviruses, reverse transcribe their gRNA into DNA, which is then transcribed into mRNA before...

...nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you’d like...

... Z, Ma X, Wang N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient genome editing...

...smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest. Promoter The promoter...

... protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable ...

...3xFLAG-tag fusion proteins. The repair template and Cas9-sgRNA plasmid is then injected into the germline of young...

...impact of off-target mutations, scientists are using sgRNA for selection and optimization. In addition, the...

...call the LADL Anchor. Using specifically designed gRNAs, they can then recruit these LADL Anchors to genomic...

...γ-Retroviral transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is...

...commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display, you...

...Through molecular biology teamwork. The guide RNA (gRNA) specifies the editing target site on the DNA, the...

...with CasRx and either an unprocessed or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating...

..., genome-wide knockout screens are possible with gRNA libraries targeting all known genes. This is a perfect...

...for Your Next Experiment Find Our List of CRISPR gRNA Design Tools ...