We narrowed to 182 results for: GRN

1. Twelve Amazing Guest Blog Contributors!

   Type

   Blog Post

   Published

   Nov. 26, 2024, 2:15 p.m.

   ...software that predicts efficiencies of prime editing gRNAs.   PRIDICT Harvinder Virk and Michael Biddle, ...

2. Performing In Vivo CRISPR Screens Using the FITS Approach

   Type

   Blog Post

   Published

   May 2, 2024, 1:15 p.m.

   ...types. A key feature of this system is a lentiviral gRNA expression vector (pRDA_526) that (1) enables efficient...

3. Addgene’s Top 10 Blog Posts from 2014

   Type

   Blog Post

   Published

   Dec. 16, 2014, 8:01 p.m.

   ...Most Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench (Guest...

4. Editor's Choice, August 2016

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   Blog Post

   Published

   Sept. 2, 2016, 1:32 p.m.

   ...the transitions at target sequences specified by gRNAs. Caveats, potential specificity issues, and other...

5. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

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   Blog Post

   Published

   Sept. 15, 2014, 6:05 p.m.

   ..., programmable DNA cutting enzyme. Second, since gRNA and crRNA could be combined into one RNA to direct...

6. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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   Blog Post

   Published

   Aug. 18, 2023, 4:15 p.m.

   ...the case of retroviruses, reverse transcribe their gRNA into DNA, which is then transcribed into mRNA before...

7. Viral Vectors 101: Parts of the AAV Transfer Plasmid

   Type

   Blog Post

   Published

   Aug. 11, 2020, 1:15 p.m.

   ...smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest. Promoter The promoter...

8. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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   Blog Post

   Published

   Sept. 14, 2021, 1:15 p.m.

   ... Z, Ma X, Wang N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient genome editing...

9. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

   Type

   Blog Post

   Published

   Nov. 12, 2019, 2 p.m.

   ... protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable ...

10. Using Addgene's New Viral Service for Your Research

    Type

    Blog Post

    Published

    Nov. 17, 2016, 3:30 p.m.

    ...nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you’d like...

11. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    Published

    July 7, 2015, 3:36 p.m.

    ...3xFLAG-tag fusion proteins. The repair template and Cas9-sgRNA plasmid is then injected into the germline of young...

12. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type

    Blog Post

    Published

    June 23, 2022, 1:15 p.m.

    ...impact of off-target mutations, scientists are using sgRNA for selection and optimization. In addition, the...

13. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type

    Blog Post

    Published

    Sept. 3, 2019, 1:15 p.m.

    ...call the LADL Anchor. Using specifically designed gRNAs, they can then recruit these LADL Anchors to genomic...

14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post

    Published

    Aug. 6, 2024, 1:15 p.m.

    ...γ-Retroviral transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is...

15. Plasmids 101: Screens vs. Selections

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    Blog Post

    Published

    Nov. 10, 2022, 2:15 p.m.

    ..., genome-wide knockout screens are possible with gRNA libraries targeting all known genes. This is a perfect...

16. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...with CasRx and either an unprocessed or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating...

17. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type

    Blog Post

    Published

    March 17, 2020, 1:32 p.m.

    ...Through molecular biology teamwork. The guide RNA (gRNA) specifies the editing target site on the DNA, the...

18. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    Published

    June 29, 2017, 1:06 p.m.

    ...commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display, you...

19. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    Published

    June 2, 2016, 2:30 p.m.

    ...for Your Next Experiment Find Our List of CRISPR gRNA Design Tools ...

20. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type

    Blog Post

    Published

    Dec. 8, 2022, 2:15 p.m.

    ...good reason. It is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it ...