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Showing: 141 - 160 of 320 results
  1. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...specific nucleotide substitutions (or deletions) in a tailored manner. The approach can be used in conventional...plasmids, but may also facilitate genome editing. Tailored mutations are commonly introduced to endogeneous...
  2. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...fluorescence for fluorescently labeled probes. Colorimetric and luminescence based detection methods are...and behavior.     References 1. Southern, Edwin Mellor. "Detection of specific sequences among DNA fragments...
  3. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...of carbohydrate binding proteins waiting to be explored and exploited. With better characterization, these...Jessica C. Sacher, and Christine M. Szymanski. "Exploring the interactions between bacteriophage-encoded...
  4. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... work The Island of Dr. Moreau (1896) where he explores the concepts of animal testing and modifications...but the common themes that these authors were exploring were space and robots. Warping to stars in space...
  5. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...effector — Cas9, Cas12, or Cas13. If you’d like to explore Class 2 CRISPR in more depth, the Jennifer Doudna...8(1). https://doi.org/10.1186/s13578-018-0255-x Taylor, H. N., Laderman, E., Armbrust, M., Hallmark, T...
  6. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...antibodies (i.e., immunofluorescence), or by a colorimetric reaction facilitated by an enzymatic reaction...Cruse JM, Lewis RE, Wang H, Geziena MT, Steven GE, Lorna J. (2004) ANTIGENS, IMMUNOGENS, VACCINES, AND IMMUNIZATION...
  7. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...Rhodamine 123 (R123) (Liu et al., 2017). In multi-color imaging, it is essential to select dyes with different...applications. Other current advancements include work exploring far-red-emitting dyes in bacterial imaging, offering...
  8. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...fluorophores can add a second layer of contrast, color contrast, to an image and thereby provide molecular...Microscopy Choosing Your Fluorescent Proteins for Multi-Color Imaging Choose the Brightest Fluorescent Protein...
  9. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...Traditional tracing compounds either produce a colorimetric product or are fluorescent. While dyes usually...ratios, making these neurons fluoresce in different colors. An individual neuron can then be traced throughout...
  10. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ...never forget! The Mackin Lab from University of Colorado -Anschutz Medical Campus (@CSD_CUAMC) caught a...
  11. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...impossible, due to their small size and inconspicuous coloring.  FLIS plasmid Recently, the Phuels lab deposited...
  12. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...coincidentally matches our Christmas tree's RGB colors) 😅🎅🤶🎄👨‍🔬👩‍🔬 pic.twitter.com/y34Tv8VCLT—...
  13. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...best costume takes home the ultimate prize – a glorious trophy. Sure it’s plastic and you have to share...
  14. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...downloading my plasmid request data, I started to explore how I could visualize the aggregated data. Plotting...
Showing: 141 - 160 of 320 results