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We narrowed to 317 results for: MUT

Showing: 141 - 160 of 317 results

  1. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...the preferred pathway for generating knock-in mutations, and NHEJ is an error-prone pathway frequently...frequently introducing frameshift knockout mutations. Thus, if genetic manipulation one way or the other (knock-in...

  2. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...example, very few diseases manifest as a single mutation or expression dysregulation event. Diseases are...multiple layers of dysregulation from either genetic mutation and/or TF dysregulation. A targeted TF screening...

  3. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...long sequences with reduced risks of unwanted mutations in mammalian cells. Long sequences traditionally...traditionally have higher risks of off-target mutations. These plasmids contain catalytically inactive dCas9 and...

  4. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...non-functional 𝛽-galactosidase. Complementing this mutation by expressing this deleted portion, for example...custom primers are especially helpful in verifying mutations that wouldn't be reached by sequencing from either...

  7. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...vector with half the size of the ssAAV genome has a mutation in the terminal resolution site (TRS) to form ...genome with wild-type ITRs at the both ends and mutated ITR at the center of symmetry. After uncoating ...

  9. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ... You’re muted on Zoom. Your video conferencing software freezes and then crashes. You’re muted on Zoom...

  10. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...gene. One such strain is DB3.1, which contains a mutant version of DNA gyrase (gyrA462) that is resistant... al. “F plasmid CcdB killer protein: ccdB gene mutants coding for non-cytotoxic proteins which retain ...

  11. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...additional SapTrap plasmids used for introducing point mutations that was published in a microPublication (Schwartz... E. M. "SapTrap vectors for introducing point mutations with unc- 119 + selection." MicroPublication 1...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...with a deletion-mutant rabies virus. Retrograde neuronal tracing with a deletion-mutant rabies virus. Pubmed...

  14. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ... if you want to knock out a gene, a frameshift mutation introduced anywhere within the early coding region... hand, if you are trying to knock-in a patient mutation or need to make a precise amino acid change, there...

  15. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...co-injection of Cas9 and a single-guide RNA targeting the mutant allele. When all was said and done, the researchers...targeted to precisely edit with the goal of ‘fixing’ mutations or introducing a desired edit, but this particular...

  16. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...with BioRender.com. Add silent mutations near point mutations to create 3-base (or longer) tracts...

  17. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...orientation limiting FRET efficiency, circular permutation (Baird et al., 1999) of the fluorescent protein...References 1. Baird, Geoffrey S., et al. "Circular permutation and receptor insertion within green fluorescent...

  18. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...genes with many modifications. We look for point mutations, truncations, and insertions that could compromise...compromise function. When we do find mutations, we check to see if they affect the translated amino acid. ...

  19. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...indicated in the “Species Other” column. Relevant Mutations Indicate the amino acid change as well as any...functional consequences of that change. For example, “Mutated aspartate 123 to Lysine (D123K), lowers biosensor...

  20. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...your supervisor’s viewpoint and brainstorm about mutually beneficial solutions Your supervisor might not... his or her viewpoint, and discuss options for mutually beneficial solutions. If you package your challenges...
Showing: 141 - 160 of 317 results