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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 329 results for: NUC

Showing: 141 - 160 of 329 results

  1. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This silent mutation...editing by sequence trace decomposition. (2014) Nucleic Acids Research Volume 42, Issue 22, Page e168. ... template-directed CRISPR-Cas9 editing. (2018) Nucleic Acids Research Volume 46, Issue 10, Page e58. https...

  2. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...confer specificity of the nuclease; however, many Cas and Cas-like nucleases are promiscuous. In other...effects? The CRISPR-Cas system is an RNA-guided nuclease that either introduces a ssDNA nick, a dsDNA break...

  3. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ... cell type, are intermingled in the same brain nucleus: a serotype that works great in a GABA neuron, ...Volumetric Spread in the CNS. Molecular Therapy - Nucleic Acids, 8, 184–197. https://doi.org/10.1016/j.omtn...AAV-Mediated Gene Transfer. Molecular Therapy - Nucleic Acids, 8, 13–25. https://doi.org/10.1016/j.omtn...

  4. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...often terminate with a poly(A). ORF2 encodes endonuclease and reverse transcriptase activities.    ...preferred target site for integration is TA dinucleotides and it leaves behind the CAG DNA footprint from... generated by directly injecting DNA into the pronuclei of fertilized eggs, which leads to random incorporation...

  5. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...that are comprised of cells with a large nucleus, large nucleoli, and little cytoplasm. Positive staining...completely removed.  Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids...

  8. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though ...restriction sites into which you can clone your 20 nucleotide guide sequence of choice (for generation of a...

  11. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Soup by Aliyah Weinstein RNA aptamers, which are nucleic acid sequences that fluoresce upon binding a small...Transfection by Alyssa Cecchetelli The introduction of nucleic acids into bacteria or eukaryotic cells is a common...

  12. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the NLS...NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress gene expression. In the...

  13. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...addition of 4-hydroxytamoxifen, it can move to the nucleus for activity. They also developed one of these ...gene activation and editing. Read the paper in Nucleic Acids Research Find the drug inducible Cas9 plasmids...

  14. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...and binds the inverted repeats (green); and an endonuclease reaction occurs which cuts the DNA.3.) The released...transposon can now bind a strand of DNA with a TA dinucleotide (there are many such sites in the human genome...

  18. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...tertiary structure when transcribed which aids in nuclear export of mRNA and helps increase the expression...The polyadenylation or polyA signal aids in the nuclear export of RNA and RNA translation, and promotes...

  19. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...green cloud) functions both as a restriction endonuclease and as a ligase by cleaving and rejoining supercoiled... 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase....

  20. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable single ...RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit a transcriptional...
Showing: 141 - 160 of 329 results