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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 456 results for: PAN

Showing: 141 - 160 of 456 results

  1. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...plant base editors. Plant CBE and ABE with expanded PAM compatibility All of the base editors mentioned.... Hua, Kai, Xiaoping Tao, and Jian‐Kang Zhu. "Expanding the base editing scope in rice by using Cas9 variants.... PubMed PMID: 29867128. 11. Li, Chao, et al. "Expanded base editing in rice and wheat using a Cas9-adenosine...

  3. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...Young. The use of CRISPR/Cas9 for gene editing has expanded since its adaptation for use in mammalian cells...ordering the sgRNA is simple and easy. Several companies supply gRNA sequences, such as GenScript, ThermoFisher...order them yourself through whatever DNA supply company you currently use. You can also find many previously...

  4. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ....  How do you find validated antibodies? Many companies that produce and distribute antibodies validate...through their publications or as a review on a company website. It is important to review the data and...can help you compare antibodies from different companies, such as Validated Antibody Database and CiteAb...

  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...recombinase variants to optimize PASSIGE efficiency (Pandey et al., 2024). The best-performing variant yielded...sequence between two attR/L sites. Image reused from Pandey et al. 2024 under CC-BY 4.0 license.   An, ...doi: https://doi.org/10.1038/s41587-021-01133-w Pandey, S., Gao, X. D., Krasnow, N. A., McElroy, A., Tao...

  6. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...modified through phosphorylation (1). However, the expansion of studies on protein phosphorylation in an array...PubMed PMID: 15790858. 5. Park, Hee-Sung, et al. "Expanding the genetic code of Escherichia coli with phosphoserine... Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156 (2013):...

  7. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...labeling compared to immunoprecipitation. The top panel depicts the identification of heat-shock proteins...in HCT116 cells (colorectal cancer). The bottom panel depicts the identification of general heat-shock...different interneuron populations. The enhancers span all four major classes of GABAergic interneurons...

  8. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...cle10ts) allele), increasing experimental options. Expanding and improving the CRISPR/Cas editing toolbox for...need to segregate away co-conversion markers. Expanding beyond Cas9 Since the development of CRISPR/Cas9...https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B (2015) Streamlined...

  9. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...allowing users to easily isolate and subsequently expand monoclonal cultures.  Interpreting flow cytometry...experimental sample. In addition, you should include a panel of “fluorescence-minus one” controls, samples stained...experiment except one. The fluorescence-minus one panel will help you set up gating during data analysis...

  10. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ... of more stable, brighter, and specific dyes, expanding the capabilities of fluorescence-based techniques...® system, the new Janelia Fluor HaloTag Ligands expand the self-labeling capabilities of this platform...Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding the microbiologist toolbox via new far-red-emitting...

  11. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...by the trial. During clinical trials, expansion of participants based on early results can enhance the...treatment regimens were not chosen carefully. Participants in clinical trials should be picked based on...

  12. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ... large sequences (up to 10 kb) into the genome (Pandey et al., 2024; Yarnall et al., 2023) Prime editing...doi.org/10.1038/s41587-021-01025-z Doman, J. L., Pandey, S., Neugebauer, M. E., An, M., Davis, J. R., Randolph...410. https://doi.org/10.1038/s41587-021-01039-7 Pandey, S., Gao, X. D., Krasnow, N. A., McElroy, A., Tao...

  13. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...within a sample to expected labeling (Figure 2). The panel of antibodies used in this approach should also ...cell tracker dyes are helpful in designing such panels, so that pre-stained cell lines can be mixed together...particularly useful when designing larger multicolour panels, where different manufacturers may have the same...

  14. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...Genome Engineering Using CRISPR/Cas Systems has expanded out of control. To further consolidate these FAQs...the donor. For example, choosing target sites that span the knock-in gene. For making mutations, one good...Laganière, J., Cheng, A. W., Hockemeyer, D., Gao, Q., Alagappan, R., Khurana, V., Golbe, L. I., Myers, R. H., ...

  15. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...cancers, including a high percentage (up to 95%) of pancreatic cancers. Ras oncogenes have been difficult to...sequence homology (usually 30-40bp overlaps). To expand the capabilities of Gibson assembly and make a ...scientists use GMAP, there will be a continuous expansion of compatible backbones, promoters, and genes ...depositing your new plasmids with Addgene to help expand the collection! The Jacks lab has made several ...

  16. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...your data by Nov. 12 to compete. The top ten participants will be invited to compete in our speed round...winner each receiving a $500 travel award. All participants will receive an Addgene t-shirt.  Our judges...

  17. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...and we have already collected over 100 entries spanning six different species and dozens of different ...performing the experiment. Every entry has an accompanying image so that you can see for yourself how the...
Showing: 141 - 160 of 456 results