...variants. This resulted in the construction of redCALWY-1, a red-shifted FRET sensor for zinc using variants... his calcium indicators can be used in mouse, Drosophila, C. elegans, and zebrafish – essentially all ...

...defunct (but fun) plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne ...Networking connections can last years This is a graphic snapshot of my LinkedIn network from a few years...

... cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis...Halstead et al. used TRICK to examine translation of Drosophila patterning gene oskar. Early in development, ...

...heterodimerizes with CIBN upon blue light exposure (see Fig 1*). When the group targeted the enhancer region of ...further develop dCas9 for synthetic looping, the Phillips-Cremins lab developed the light-activated-dynamic-looping...

...vector-exosomes), exosome AAVs, or exo-AAVs (Figure 1). The researchers discovered that these particles ...coagulation factor IX in liver in a mouse model of Haemophilia B (Meliani et al., 2017). Exo-AAV9 was successfully...

...individual protein has all the components necessary to: 1. Bind to a guide RNA The guide RNA, which will be ...meningitidis (NM) 3' NNNNGATT  Streptococcus thermophilus (ST) 3' NNAGAAW  Treponema denticola (TD...

...and also by a lactose analogue, IPTG (isopropyl β-D-1-thiogalactopyranoside). (To be completely accurate...reproduce to form colonies (used to complement auxotrophic cell lines), or genes which rescue a lethal phenotype...

...region: IgM, IgD, IgG, IgE, and IgA.    Figure 1: Diagram of an antibody labeled with Fc, Fab, heavy...found bound to IgE receptors on the surface of basophils and mast cells, two types of white blood cells...

...and CasPEDIA have got you covered!     Fig. 1 – CasPEDIA Cas ID display information for a given ...to relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas...

... UK team members before they log off for the day. 1:45 PM--Write final report. Work on a final report ...what the day to day looks like. Check out the infographic for an outline of my day or find the text version...

...prove the technology. This transition takes around 1 – 2 years. The product is the measure of success...Business - Posted on Nature.com, contributed by Julia Phillips and Fiona Beckman Acknowledgments Thank you ...

... trafficking of these biosensors.   Figure 1: Schematic of iGluSnFR3 (left). GluSnFR3 in pMinDisplay... calcium signaling related fluorescence during morphine administration and withdrawal to explore how these...

...see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ...

...first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple catalogue...follow him on Twitter @CMacPhD.          References  1. Ghorbal, Mehdi, et al. "Genome editing in the human... not. It is an offline tool, but comes with a graphical user interface. Best strategy for labs: Define...

...significant progress in clinical trials.     Figure 1: Types of delivery and editing strategies for CRISPR...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...

...viruses - 2 cas systems regulate at the DNA level and 1 at the RNA level. Udi Qimron from Tel Aviv University...invasion.  His lab is studying P. furiosus, a hyperthermophile that exists in sea vents at the boiling point...

... – a mechanical device that could be used to bend 1/8th inch steel wire into a backbone structure of a...popularity was short-lived as powerful molecular graphics packages were soon developed that allowed researchers...

...target DNA quickly and accurately.  Figure 1: Steps in PCR reaction   This process of denaturation...relies on heat resistant DNA polymerase from the thermophilic bacterium, Thermos aquaticus (Taq). Taq polymerase...

...editing machinery such as CRISPR-Cas. Figure 1: Schematic of targeted gene knock-out (left) and targeted... WM, Curiel DT (2019) Long-term correction of hemophilia B using adenoviral delivery of CRISPR/Cas9. Journal...

...genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse genome...shown that a Cas9 protein from Geobacillus stearothermophilus is more stable in human plasma than the standard...