We narrowed to 193 results for: SPL

1. The time and cost required to make a plasmid

   Type

   Blog Post

   Published

   March 23, 2023, 1:15 p.m.

   ...same time period. For ease of calculations*, let’s split the difference and put scientific inflation at… ...

2. CRISPR 101: Cas9 vs. The Other Cas(s)

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   Blog Post

   Published

   Dec. 8, 2022, 2:15 p.m.

   ...cleavage and 2). cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less...

3. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   Blog Post

   Published

   March 19, 2019, 1:31 p.m.

   ...the Uppalapati and Woolley lab developed a phage display technique using small scaffold proteins to identify...

4. Choosing the B(right)est Fluorescent Protein: Photostability

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   Blog Post

   Published

   June 8, 2017, 2:30 p.m.

   ...top of photobleaching, fluorescent proteins may display reversible intensity changes (Shaner et al, 2008...

5. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   Blog Post

   Published

   Dec. 16, 2015, 3:30 p.m.

   ...SpCas9 N692A/M694A/Q695A/H698A). This new variant displays comparable on-target activity and similar or decreased...

6. Viral Vectors 101: Virus Safety

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   Blog Post

   Published

   June 22, 2023, 1:15 p.m.

   ...or face shields are required whenever there’s a splash or spray risk in BSL-1. In BSL-2 labs, eye protection...

7. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ...chicken beta actin promoter, and rabbit beta-globin splice acceptor, contains sequence that is over 80% GC...

8. Progress Towards a PAM-Free CRISPR

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   Blog Post

   Published

   March 7, 2024, 2:39 p.m.

   ... reliability. Notably, the SpRY variant didn’t display higher off-target effects than SpCas9, a pleasant...

9. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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   Blog Post

   Published

   Nov. 7, 2017, 1:59 p.m.

   ...direct evolution, capsid shuffling, and peptide displays. These capsids will be selected both for their...

10. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...no detectable editing. Additionally, off-target spleen editing was not detected and Nanoblade injection...

11. Overcoming the Challenges of Lentiviral Production

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    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...reduced. Third generation packaging systems further split the viral genome by removing the viral Rev gene ...

12. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ...purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral genes and transgene...

13. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ... cleavage. Now, two new studies show that Cpf1 displays lower off-target editing than Cas9, confirming...

14. Of Myc and Men

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    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ... E (2020) The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent ...

15. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...) and to see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the ...

16. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    Blog Post

    Published

    Feb. 7, 2019, 2:16 p.m.

    ...happens often because the HDR inserted sequence splits up the guide sequence.) If neither of the above...

17. Cloning Mammalian Cells with the Agarose Method

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    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...efficiency is not known, this is easily achieved by splitting transfected cells into 3 plates at different densities...

18. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post

    Published

    Feb. 23, 2016, 3:30 p.m.

    ...clones, they found that 80% and 50% of clones displayed proper insertion at the 5’ and 3’ junctions, respectively...

19. Summer SciComm Series: Modes of Communication

    Type

    Blog Post

    Published

    July 1, 2022, 1:15 p.m.

    ...  No visuals. Variety of hosting platforms can splinter audience. Primarily digital distribution (though...

20. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    Published

    Dec. 3, 2019, 11:38 a.m.

    ...the number of plasmids matching each filter is displayed in parentheses.  In this example I wanted to find...