We narrowed to 193 results for: SPL

...the Uppalapati and Woolley lab developed a phage display technique using small scaffold proteins to identify...

...the nerve to do what I did. Until that panicked, split-second decision, I had always thought I had an obligation...

...same time period. For ease of calculations*, let’s split the difference and put scientific inflation at… ...

...cleavage and 2). cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less...

...SpCas9 N692A/M694A/Q695A/H698A). This new variant displays comparable on-target activity and similar or decreased...

...direct evolution, capsid shuffling, and peptide displays. These capsids will be selected both for their...

...reduced. Third generation packaging systems further split the viral genome by removing the viral Rev gene ...

...purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral genes and transgene...

...no detectable editing. Additionally, off-target spleen editing was not detected and Nanoblade injection...

...or face shields are required whenever there’s a splash or spray risk in BSL-1. In BSL-2 labs, eye protection...

...chicken beta actin promoter, and rabbit beta-globin splice acceptor, contains sequence that is over 80% GC...

... reliability. Notably, the SpRY variant didn’t display higher off-target effects than SpCas9, a pleasant...

... cleavage. Now, two new studies show that Cpf1 displays lower off-target editing than Cas9, confirming...

...) and to see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the ...

...efficiency is not known, this is easily achieved by splitting transfected cells into 3 plates at different densities...

... E (2020) The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent ...

...happens often because the HDR inserted sequence splits up the guide sequence.) If neither of the above...

...clones, they found that 80% and 50% of clones displayed proper insertion at the 5’ and 3’ junctions, respectively...

...the number of plasmids matching each filter is displayed in parentheses.  In this example I wanted to find...

...  No visuals. Variety of hosting platforms can splinter audience. Primarily digital distribution (though...