Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 351 results for: SUA

Showing: 141 - 160 of 351 results

  1. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...protein or disruption of a gene, can be easily visualized in a living organism from embryonic development...adulthood. This ability to genetically manipulate and visualize cells and tissues allows scientists to conduct...

  3. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...multimers, since restriction sites in multimers usually repeat in tandem along with the repeated plasmid...multimer in your sample remains unclear after visualizing it on a gel, one of the other methods below should...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...fragments by utilizing regions of sequence homology (usually 30-40bp overlaps). To expand the capabilities of...lab yeast strains since they are prototrophic, usually have low levels of homologous recombination, and... challenging. Small molecule kinase inhibitors usually target soluble kinase domains, but these domains...

  5. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...the key to a great image is contrast. The human visual system relies primarily on contrast to identify... is collected and focused onto a camera for visualization. Illuminating a sample in the opposite direction...

  7. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...Grade DNA (high quality/low quantity) – You can usually complete a cloning experiment with a relatively...you flow lysate and buffers through the matrix, usually by centrifugation or vacuum. In a bead-based approach...

  9. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...In all cases, structural biology can help you visualize the system you’re working with and better understand...developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California...

  10. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...by testing them. For intracellular targets, we usually test 3 different fix/perms (methanol, PFA-saponin...lines using these two antibodies. This is the most usual level of evidence supplied to support the suitability...

  13. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...remove virus from cells Plasmid Non-integrating, usually Medium Medium Generates transgene-free and vector-free...lower levels of efficiency at generating iPSCs, usually several orders of magnitude lower than retroviruses...

  14. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...samples, and treatments may affect the assay. It’s unusual to need to adjust amounts of reagents in your chemistry...reference genes deserves more attention than it usually receives. Different reference genes have shown ...

  15. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ... look at various CRISPR applications (such as visualize, screen, activate, and repress) on Thursday. We...

  16. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...ExpressionAuthors: Marjorie Buss and Mengtong "Tom" DuanVisualizing Your Plasmid Request Data Author: Joachim Goedhart...

  20. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...Modifying the DNA sequence of an organism’s genome usually for a research or applied goal. Components of CRISPR...
Showing: 141 - 160 of 351 results