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Showing: 141 - 160 of 520 results

  1. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ... super family). To overcome these challenges, we set out to develop a hassle-free, highly efficient Cas9...eLife 2018;7:e35069 DOI: 10.7554/eLife.35069. How to set up csRNP knock-in experiments If your lab is new ...experiments, there is no need to worry at all. Setting up tag knock-in experiments using the csRNP method...

  2. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...or "Unpublished or pre-publication plasmids" and set a desired distribution status. You can submit "Published... below:   Pooled Library A pooled library is a set of plasmids all built with the same backbone and ...entered will be saved and the page will open a new set of entry fields to describe the next insert. Repeat...

  3. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...by the lab of Joachim Wittbrodt might be just the set of tools that you need. The Golden GATEway cloning...which signaling events occur in a physiological setting. Genetic techniques typically rely on overexpression...RF-1; Bacterial strain EcAR7) and minimizing the set of plasmids required to make singly or multiply phosphorylated...between. The MoClo system is comprised of three sets of cloning vectors (Level 0, 1, or 2) which can ...Additionally, the lab recently described the use of another set of mGRASP plasmids in their Neuron 2014 paper. ...sequence to be integrated and a removable integrase cassette. They validated this methodology in two differents...expression and purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor...

  4. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ... with GFP Kevin White’s lab recently deposited a set of CRISPR plasmids to tag human transcription factors...

  5. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria, fungi...

  7. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ... kills you, but the cytokine storm it ultimately sets off.  The virus works its way into immune cells ...

  8. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...are ready to begin the screening process. Want to set yourself up for a screening success? Before you start...look for off-target changes. This method requires a set of control cells to compare the sequencing reads ...

  9. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...newly synthesized DNA strand. Each degenerate primer set contains three consecutive randomized bases (NNN)...NNN) at a given codon site and multiple primer sets tile across the protein or region of interest. A low...

  10. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...-day experiment in the lab. You have two ways to set up the experiment. The first way, it will take you... project (with metrics if possible) and, at the outset of a task, I outline the activities that are needed...

  11. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...from someone seeing me speak in a non-interview setting. There are many resources on self-promotion (how...of this article will be talks for non-academic settings. Many of these guidelines will apply to any type...

  12. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...contamination with RNase leading to degradation that can set you back days. Working with plasmid DNA is much easier...pCAG-EGXXFP plasmid? They are the perfect primer sets to use to genotype your pups. A simple PCR with ...contains humanized Cas9 expression cassette and sgRNA expression cassette. The target sgRNA can be cloned...of validation for DSB mediated EGFP expression cassette reconstitution. When the target sequence was digested...took place and reconstituted the EGFP expression cassette. (b) pCAG-EGxxFP target plasmid and pX330-sgRNA...

  14. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...while keeping the party going! At Addgene, we’ve set-up an exhibitor booth in order to help scientists...

  16. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ... recently deposited “Mobile-CRISPRi” plasmids, a set of modular CRISPRi tools that can be used in diverse...

  19. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...the biggest hurdles for scientific sharing. So we set out to streamline this process. We take pride in ...
Showing: 141 - 160 of 520 results