...by the lab of Joachim Wittbrodt might be just the set of tools that you need. The Golden GATEway cloning...which signaling events occur in a physiological setting. Genetic techniques typically rely on overexpression...RF-1; Bacterial strain EcAR7) and minimizing the set of plasmids required to make singly or multiply phosphorylated...between. The MoClo system is comprised of three sets of cloning vectors (Level 0, 1, or 2) which can ...Additionally, the lab recently described the use of another set of mGRASP plasmids in their Neuron 2014 paper. ...sequence to be integrated and a removable integrase cassette. They validated this methodology in two differents...expression and purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor...

... with GFP Kevin White’s lab recently deposited a set of CRISPR plasmids to tag human transcription factors...

...with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria, fungi...

... are sharing what they call “raw science” – data sets, code, and, I think it’s safe to say all of us here...

... kills you, but the cytokine storm it ultimately sets off.  The virus works its way into immune cells ...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...-day experiment in the lab. You have two ways to set up the experiment. The first way, it will take you... project (with metrics if possible) and, at the outset of a task, I outline the activities that are needed...

...newly synthesized DNA strand. Each degenerate primer set contains three consecutive randomized bases (NNN)...NNN) at a given codon site and multiple primer sets tile across the protein or region of interest. A low...

...are ready to begin the screening process. Want to set yourself up for a screening success? Before you start...look for off-target changes. This method requires a set of control cells to compare the sequencing reads ...

...from someone seeing me speak in a non-interview setting. There are many resources on self-promotion (how...of this article will be talks for non-academic settings. Many of these guidelines will apply to any type...

...contamination with RNase leading to degradation that can set you back days. Working with plasmid DNA is much easier...pCAG-EGXXFP plasmid? They are the perfect primer sets to use to genotype your pups. A simple PCR with ...contains humanized Cas9 expression cassette and sgRNA expression cassette. The target sgRNA can be cloned...of validation for DSB mediated EGFP expression cassette reconstitution. When the target sequence was digested...took place and reconstituted the EGFP expression cassette. (b) pCAG-EGxxFP target plasmid and pX330-sgRNA...

...energy, Martí Aldea and collaborators have created a set of “innocuous tags” (inntags) less likely to alter...

...while keeping the party going! At Addgene, we’ve set-up an exhibitor booth in order to help scientists...

... recently deposited “Mobile-CRISPRi” plasmids, a set of modular CRISPRi tools that can be used in diverse...

...endeavor.  Break the process down into steps and set aside time during the week to tackle each one.  Get...

...  Cloning grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant...

...careers. Failure to strengthen the talent pipeline sets the world up for reduced impact of scientists and...spearheaded by Cynthia Fuhrmann at University of Massachusetts Medical School. It has been supported by a Steering...

...the biggest hurdles for scientific sharing. So we set out to streamline this process. We take pride in ...

... Mix & Go! E. coli Transformation Kit and Buffer Set  from Zymo Research to make competent cells because...

...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ...library that contains up-to-date information and data-sets, as well as an interactive gRNA viewer. The Moffat...address this problem, Csaba Pál’s lab has created a set of vectors that allows one to use the MAGE method...method in unmodified bacterial species (4). This set of plasmids (dubbed pORTMAGE) expresses the λ Red recombinase... kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository. The... unique tool with their Yeast Prototrophy Kit, a set of plasmids designed to complement unused auxotrophies...Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles...

...in leukemia, the Lowe lab was able to identify a set of mutations that made cancer cells more resistant...systems check out Gerald Rubin’s lab who created a set of modular vectors that can be used to optimally ...