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We narrowed to 608 results for: Sis;

Showing: 141 - 160 of 608 results

  1. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...The researchers identified MerMAIDS from their analysis of different publicly available metagenomic data... study demonstrates the power of combining the analysis of publicly available metagenomic datasets with...fused dCas9 to a truncated CIB1 protein from Arabidopsis (CIBN), which they call the LADL Anchor. Using... iterations is the addition of CRY2, another Arabidoposis protein that heterodimerizes with CIBN upon ...

  2. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ... lost a specific gene survive (ex: antibiotic resistance marker). In a previous blog post, we covered ...plasmid backbone, as it often relies on antibiotic resistance. But what about identifying clones that contain...Lynne Postovit’s lab. It contains an ampicillin resistance marker in the backbone. A selection on agar containing...visualize this process, or visit our restriction analysis blog post. Colony PCR Colony PCR can detect...

  3. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...mechanism by which bacteria have gained antibiotic resistance and virulence. Scientists have studied how HGT... replication-incompetent phage particles with assistance from a ‘helper’ phage prior to transduction. ...can infer genetic advantages such as antibiotic resistance. Bacterial conjugation as explained by...Bacterial Conjugation. In An Introduction to Genetic Analysis. 7th edition (2000). Meibom, Karin L., et al. ...

  4. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...dosing, we advise that users keep titering method consistent across samples within a project. Targeting Your... on vector dose. The color diversity in Vector Assisted Spectral Tracing (VAST) experiments using PHP....best practices’ for model organisms.    Q11: How consistent is expression is when injecting CAP-B10 or CAP-B22...linked to epigenetic changes, such as multiple sclerosis?  A: Basic science and preclinical researchers...

  5. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...of common plasmid features such as antibiotic resistance genes, selectable markers, promoters, origins...plasmid sequence For more immediate, high-level analysis, you can click directly on the “Analyze Sequence...to additional tooltip information and sequence analysis tabs including: Maps This is the first tab...For example, if you hover over the ampicillin resistance gene, you’ll find the full name of the gene, ...

  6. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...expression profiling, systematic tumorigenesis assays, drug epistasis assays, and morphological profiling...huge challenges preventing us from testing this hypothesis: There was no large scale, broadly available... corresponding to cancer variants derived from analysis of lung adenocarcinoma genomes (Lung Cancer Mutant...

  7. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...newly synthesized product from a site-directed mutagenesis experiment? The answer to both questions is the...basepairs or small indels that occur during DNA synthesis, and protomoting or repressing protein expression...cleave DNA. DpnI is often used for site directed mutagenesis. During this process, incorporation of a desired...Troubleshoot Your Sequencing Results Use REPLACR Mutagenesis to Mutate Your Plasmids Resources on Addgene.org...

  8. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...will catch you up on the core replication and resistance features of yeast vectors and explain how they... and diploid state which is useful for genetic analysis, as well as an efficient mechanism of homologous... due to high rates of spontaneously occurring resistant mutants and the insensitivity of yeast strains...auxotrophic markers. The MX series of antibiotic resistance cassettes is most commonly employed, with the...

  9. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...Protein turnover is the combined effect of protein synthesis and degradation. These processes control the abundance...possible to visualize the localization of protein synthesis. Alternatively, fluorescent protein tags can be...the system. Another method for studying protein synthesis in situ is the knock-in of fluorescent tags into...Nathans, Daniel. "Puromycin inhibition of protein synthesis: incorporation of puromycin into peptide chains...

  10. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...that is resistant to the toxic effects of CcdB. Another commercially available, CcdB-resistant strain ... investigator’s gene of interest.   CcdB resistant E. coli strains complete the system But ccdB alone...contains the F plasmid (F’ strains) will also be resistant to CcdB, as the native ccdA will be present. Most...

  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ... of replication, protein tags, and antibiotic resistance markers (just to name a few). In this edition...site-specific recombination events. The system consists of two components derived from the P1 bacteriophage...sites are 34-base-pair long recognition sequences consisting of two 13-bp long palindromic repeats separated...targeting, targeted clones are selected for using a resistance marker; however, it is often desirable to remove...

  12. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...gotten myself.” Perkins noted that there is a more emphasis today about non-academia and industry jobs. Since...her first job at Addgene as an Order Processing Assistant. After a few months, she left school when she ...mean you’ve failed. “I got a job as a research assistant in a lab at the university that I went to. Making.... She was working as a research and technical assistant, when her life changed––she had a daughter. Needing...

  13. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ... 1000000097) The Winslow lab barcoding library consists of 96 retroviral plasmids (based on the Murine...high-throughput sequencing to perform multiplexed analysis of cells pre-treated with a compound of interest...Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus? Get the ...mammalian cells. The MuLE system kit from the Frew lab consists of 91 building block Entry vectors and 5 lentiviral...

  14. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...makes them ideal candidates for applications in oncolysis and vaccination!) In the 65 years since its discovery...virion is a non-enveloped particle of ~100 nm, consisting of a capsid surrounding an inner core which contains...adenovirus: the virion is ~ 100nm in size, and consists of an icosahedral capsid made of 3 types of proteins... for creating adenoviral vector constructs. It consists of 2 plasmids: a shuttle vector (in which the ...

  15. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...generating huge amounts of data that could provide the basis for student projects in introductory labs. Genome...evolution of the genes involved in methionine synthesis (5). The budding yeast, Saccharomyces. cerevisiae...deletion strains is available (6). Methionine synthesis in S. cerevisiae (Figure 1) occurs through a well-characterized...cerevisiae genome by gene deletion and parallel analysis. Science 285, 901-906. (1999). PubMed PMID: 10436161...

  16. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...Kleckner. "Recombination, pairing, and synapsis of homologs during meiosis." Cold Spring Harbor perspectives...Special breeding cases Whether your mouse colony consists of genetically engineered mice with a single mutation...chromosome. Homologous chromosomes recombine during meiosis, creating gametes that contain a mix of genetic...

  17. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...the mRNA molecule, forming a ribosome complex consisting of the translated protein (sybody), ribosome,...constructed, they are used to produce a phage library consisting of phages that display the candidate sybodies...tag-free proteins for further characterization and analysis. In a rapid response to the COVID-19 pandemic,...Schepens B, Saelens X, McLellan JS (2020) Structural Basis for Potent Neutralization of Betacoronaviruses by...

  18. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...Patrizia, et al. "Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis, and gene expression...Additional passages will allow your cells to achieve a consistent growth rate. For every minute spent organizing... 1. Yin, R., et al. "Gene expression profiling analysis of bisphenol A-induced perturbation in biological...

  19. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...customization with strain-specific promoters, antibiotic resistance selection markers, gRNAs, and dCas9 proteins....Mobile-CRISPRi can be used to identify antibiotic resistance genes. Genes that are targets of antibiotics ...essential genes such as those involved in antibiotic resistance. Are you ready to move Mobile-CRISPRi into your...30675034. Peters, Jason M., et al. "Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi." Nature...

  20. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...plants that inherited an edited gene to confer resistance to the herbicide chlorsulfuron (Homo line 2) ... to drive prime editing in both tomatoes and Arabidopsis, suggesting the improvements may be broadly applicable...enables heritable desired edits in tomato and Arabidopsis. Nature Plants, 10(10), 1502–1513. doi: https...the formation of Gα-GTP by BRET. The biosensor consists of a YFP-tagged G protein and a NanoLuc® (Nluc...
Showing: 141 - 160 of 608 results