We narrowed to 542 results for: TIL
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TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....seeking a first job after the academic bench. Utilizing existing relationships as part of the job application...you a much better chance at an interview –but you still need to nail the job yourself. See the links in...
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London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m....been there to answer your plasmid questions. But until now, you’ve always had to call the United States...and depositing (all while our US counterparts are still sleeping). Plasmid sharing in Europe: Addgene’s ... -
Antibodies 101: Affinity Tags
TypeBlog PostPublishedSept. 19, 2023, 1 p.m....cleaved off from the fusion protein while it is still bound to the glutathione resin, eliminating the ... proteins in mammals. Mammalian purification is still possible but requires optimization and often more... -
Antibodies 101: Fc Effector Functions
TypeBlog PostPublishedMay 18, 2023, 1:15 p.m....process known as phagocytosis. Other functions We’re still learning about Fc effector functions and all that...driving particular cells or responses. And there are still antibody isotypes and Fc receptors that we don’t... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog PostPublishedMay 2, 2023, 1 p.m....endogenous function of the gene as a result but will still allow for expression pattern monitoring. Enhancer...insertion into an exon, like promoter traps, it will still likely disrupt the gene it monitors due to transcriptional... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog PostPublishedNov. 1, 2017, 2:04 p.m....curriculum development team at eScience Labs. She still gets to write curriculum, but has taken on other...responsibilities.“I’d say maybe 25% of my day is still what I did as an SME, and then 75% of the time I... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m....activity is also drawn out as drugs can remain present until cleared by the body. Administering the drug once...Designer Drugs): Chemogenetic Tools with Therapeutic Utility. Annual Review of Pharmacology and Toxicology 55... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....Until recently, there were no completely genetic tools that would allow researchers to label just a fraction... Applications for SPARC SPARC technology is versatile and can be applied across cell types and organisms... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog PostPublishedNov. 1, 2016, 2:30 p.m....and transformation. While restriction cloning is still routinely performed by many labs, a variety of other... of cloning has its advantages, most scientists still encounter several struggles with these techniques... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m....response to tissue damage, have excessive bone growth until eventually the patient loses mobility. Attempts ...novel solutions to treat rare diseases like FOP until a cure is available. Addgene and AJ's Avengers This... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog PostPublishedSept. 14, 2017, 12:40 p.m....but not thermophilic (high heat loving) bacteria. Until recently the only available Cas9 proteins were isolated... GeoCas9 RNPs to be incubated at high temps but still maintain activity following heat treatment. The ... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....antibody fragment was exciting, and it wasn’t long until the field was off and running with camelid-derived...So thank you to the camelids who inspired this versatile family of tools, the applications they have already... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog PostPublishedJuly 2, 2015, 8:37 p.m....analytical thinking, time management, information distillation, etc) are transferable to many different fields...thinking about preparing yourself for a career while still in graduate school, you’ll be well suited to follow... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....isotype conversion is routinely used to increase the utility of recombinant antibody tools, the resulting chimera...post has answered all of your questions about the utility of isotype conversions and chimeric antibodies!... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....the fetal tissue which HEKs were isolated from is still a mystery, but it’s currently assumed that the cells... on another cell or organism. This pipeline is utilized to transduce cell lines for basic science research... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....cabbage and kale to name a few. Despite Arabidopsis’ utility to science, and its well-known edible relatives...include growing plants require pots, trays, soil, fertilizer, seeds and a growth space with fluorescent lights... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....scientific or science communication. A Tweet is still considered scientific communication if the language...non-jargon in the context of disease testing, it can still be considered jargon in most other uses. Many laypeople... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog PostPublishedSept. 17, 2020, 1:15 p.m....Addgene plays in accelerating research. Yet this was still not enough to ensure we could keep up with demand...organizations to request support for our operations until our normal work resumed. Addgene distributes and... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m.... to visualize individual/non-overlapping cells. Until now, tools for doing this were labor-intensive and...intermediate (12%), and sparse (4%) levels. SPARC is a versatile system that can be used in many cell types and... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m....injection. How long post-injection did you wait until performing the experiment? What detection methods...data. Please note that the data hub is currently still in beta version, so we’re always looking for ways...