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We narrowed to 546 results for: TIL;

Showing: 141 - 160 of 546 results

  1. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...This post was written jointly by Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens ...grow. This post was written jointly by Brook Pyhtila and Nicole Waxmonsky. References McDade J, Waxmonsky...

  2. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...seeking a first job after the academic bench. Utilizing existing relationships as part of the job application...you a much better chance at an interview –but you still need to nail the job yourself.   See the links in...

  3. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...been there to answer your plasmid questions. But until now, you’ve always had to call the United States...and depositing (all while our US counterparts are still sleeping). Plasmid sharing in Europe: Addgene’s ...

  5. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...but not thermophilic (high heat loving) bacteria. Until recently the only available Cas9 proteins were isolated... GeoCas9 RNPs to be incubated at high temps but still maintain activity following heat treatment. The ...

  6. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...antibody fragment was exciting, and it wasn’t long until the field was off and running with camelid-derived...So thank you to the camelids who inspired this versatile family of tools, the applications they have already...

  7. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...cleaved off from the fusion protein while it is still bound to the glutathione resin, eliminating the ... proteins in mammals. Mammalian purification is still possible but requires optimization and often more...

  8. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...process known as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that...driving particular cells or responses. And there are still antibody isotypes and Fc receptors that we don’t...

  9. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...endogenous function of the gene as a result but will still allow for expression pattern monitoring. Enhancer...insertion into an exon, like promoter traps, it will still likely disrupt the gene it monitors due to transcriptional...

  10. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...activity is also drawn out as drugs can remain present until cleared by the body. Administering the drug once...Designer Drugs): Chemogenetic Tools with Therapeutic Utility. Annual Review of Pharmacology and Toxicology 55...

  11. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...Until recently, there were no completely genetic tools that would allow researchers to label just a fraction... Applications for SPARC SPARC technology is versatile and can be applied across cell types and organisms...

  12. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...and transformation. While restriction cloning is still routinely performed by many labs, a variety of other... of cloning has its advantages, most scientists still encounter several struggles with these techniques...

  13. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...response to tissue damage, have excessive bone growth until eventually the patient loses mobility. Attempts ...novel solutions to treat rare diseases like FOP until a cure is available. Addgene and AJ's Avengers This...

  16. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...are incredibly useful, chemical fluorophores can still be used in a wide variety of experiments. Read more...and to find a video of them in action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated...

  17. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...researchers lamenting the viral production process while still ordering viral vectors. Virus production can be ...Beginners Lentiviral Vector Uses and Overview AAV: A Versatile Tool for Gene Expression in Mammals Additional...

  18. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ... Molecular basis of transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into...are non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform...

  19. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...isotype conversion is routinely used to increase the utility of recombinant antibody tools, the resulting chimera...post has answered all of your questions about the utility of isotype conversions and chimeric antibodies!...

  20. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...the fetal tissue which HEKs were isolated from is still a mystery, but it’s currently assumed that the cells... on another cell or organism. This pipeline is utilized to transduce cell lines for basic science research...
Showing: 141 - 160 of 546 results